Ameliorative potential of dietary supplements, ZnO-K, citrus essential oil, and pumpkin seed oil, on sperm quality in Nile tilapia: Insights from CASA, DNA integrity, antioxidant enzymes, and gene expressions

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Sperm quality improvement is crucial to achieving the reproductive efficiency of Oreochromis niloticus. This study examined effect three dietary antioxidant supplements, kaolinite-doped zinc oxide (ZnO-K), citrus essential oil (CEO), and pumpkin seed (PSO), on sperm quality. Integrated examination tools, including Computer Assisted Semen Analysis (CASA) parameters, spermatozoa DNA integrity, enzyme bioassays, gene expressions, were applied validate One hundred ninety-two adult males (mean weight 421.31 ± 6.26 g) divided into four groups, each with replicates. The first control group was fed a diet without supplements. second ZnO- K-containing (0.06 g kg^{− 1}); third CEO-containing (10 fourth PSO-containing (15 1). ZnO-K supplementation significantly elevated milt volume (1.40 0.10 ml) concentration (5.676 x 10⁹ ml^{− 1}), as well enhancing CASA motility, velocities, integrity. An increase in activities enzymes, catalase, CAT, glutathione peroxidase, GPX, superoxide dismutase, SOD, observed ZnO-K-feeding group, recording 47.333 1.452 U 1 milt, 65.667 5.547 mU 60.667 3.382 respectively. Notably, upregulation expressed genes, HSP70, CC chemokines recorded sperms from ZnO-K- CEO-feeding compared expression suppression PSO-feeding group. All these findings suggest that CEO are efficient O. niloticus sperm, most pronounced effects shown by ZnO-K.

Язык: Английский

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Initial assessment of the toxicologic effects of leachates from 3-dimensional (3-D) printed objects on sperm quality in two model fish species

Aquatic Toxicology, Год журнала: 2023, Номер 256, С. 106400 - 106400

Опубликована: Янв. 21, 2023

Язык: Английский

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Transitioning from a research protocol to a scalable applied pathway for Xenopus laevis sperm cryopreservation at a national stock center: The effect of cryoprotectants

Journal of Experimental Zoology Part B Molecular and Developmental Evolution, Год журнала: 2023, Номер 342(3), С. 291 - 300

Опубликована: Ноя. 20, 2023

Abstract Sperm cryopreservation is a critical tool for safeguarding and managing valuable genetic resources. Protocols of Xenopus laevis sperm were available but lacking quality evaluation scalability the outcomes inconsistent. The goal this study was to begin developing center‐level pathway species by integrating French straws as containers that would facilitate germplasm repository development. objectives analyze effect of: (1) three concentrations (33, 50, 100 × 10 6 sperm/mL) on post‐thaw fertilization, (2) final (2.5%, 5%, 10%) dimethyl sulfoxide, methanol, dimethylformamide (DMFA) membrane integrity fresh frozen samples, (3) two (5% DMFA with without 5% sucrose at four cooling rates (5, 10, 20, 40°C/min) motility, (4) egg exposure different fertilization. Few differences in viability found among samples incubated cryoprotectants, thawed methanol or presented higher integrity. Samples 10% 20°C/min showed (60 ± 7%) than other rates, same total motility (30 10°C/min. Higher (10%–13%) detrimental embryo development compared lower (<6%). This provided reliable protocol yield an application potential high throughput can be used roadmap work species.

Язык: Английский

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A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS)

Micromachines, Год журнала: 2023, Номер 14(7), С. 1310 - 1310

Опубликована: Июнь 26, 2023

Sperm motility analysis of aquatic model species is important yet challenging due to the small sample volume, necessity activate with water, and short duration motility. To achieve standardization sperm activation, microfluidic mixers have shown improved reproducibility over activation by hand, but challenges remain in optimizing simplifying use these microdevices for greater adoption. The device described herein incorporates a novel micromixer geometry that aligns two inlet streams modified herringbone structures split recombine at 1:6 dilution water rapid consistent initiation polydimethylsiloxane (PDMS) chip can be operated positive or negative pressure configuration, allowing simple micropipettor draw samples into rapidly stop flow. was optimized not only zebrafish also enables practical standard computer-assisted (CASA) systems. could other differing cell sizes adopted an open hardware approach using 3D resin printing where users revise, fabricate, share designs improve across laboratories repositories.

Язык: Английский

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Enhancing evaluation of bull fertility through multivariate analysis of sperm

Journal of Dairy Science, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

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Advancing nuclear transfer cloning in zebrafish (Danio rerio) into a translational pathway using interdisciplinary tools

PLoS ONE, Год журнала: 2024, Номер 19(12), С. e0312672 - e0312672

Опубликована: Дек. 30, 2024

The Zebrafish International Resource Center (ZIRC) is an NIH-funded national stock center and germplasm repository that maintains distributes genetically modified wild-type zebrafish ( Danio rerio ) lines to the biomedical research community. ZIRC its community would benefit from incorporating somatic cell nuclear transfer (SCNT) cloning which allow preservation of diploid genomes. goal this study was advance a SCNT protocol into reproducible community-level pathway by use process mapping simulation modeling approaches address training requirements, constraints, quality management gaps. Training, for most steps in protocol, could be completed within two months; however, involved micromanipulation eggs required more than four months training. Dechorionation embryos egg were identified as major constraints because processes performed manually advanced operator manual skills. Chemical dechorionation microfluidic devices aid ways eliminate these constraints. Finally, control record initial collected recommended prevent production defects harmonize across multiple facilities. By beginning enhance reproducibility pathway, technique can implemented facilities work with other models.

Язык: Английский

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A 3-D printed vitrification device integrated with French straws

HardwareX, Год журнала: 2022, Номер 12, С. e00366 - e00366

Опубликована: Окт. 1, 2022

Язык: Английский

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