Frontiers in Genetics,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 26, 2025
Leeches
are
well
known
for
blood-feeding
habits
and
widely
used
medicinal
purposes
as
they
secrete
various
antithrombotic
substances.
However,
some
leeches
exhibit
non-hematophagous
their
significance
use
is
controversial.
Here
we
provide
the
chromosome-level
genomes
of
two
leeches,
Whitmania
acranulata
laevis,
and,
in
combination
with
previous
results
from
pigra,
compared
these
an
emphasis
on
biomolecules.
All
three
species
had
same
chromosome
number
11.
The
genome
size,
repeat
site
percentage,
protein-coding
genes
W.
laevis
(173.87
Mb,
28.28%,
23,818)
were
similar
to
those
pigra
(169.37
27.02%,
24,156),
whereas
values
(181.72
29.55%,
27,069)
higher
than
other
leeches.
was
a
monophyletic
clade
paraphyletic
relationship
pigra.
(N
=
76)
that
79),
102)
apparently
more
such
genes.
Of
21
gene
families,
9
11
differentially
expressed
respectively.
expression
profiles
families
between
laevis.
Although
there
several
cases
loss
or
pseudogenization,
most
intact
transcribable.
These
valuable
insights
into
evolution
development
drugs.
Cell Reports,
Год журнала:
2024,
Номер
43(3), С. 113909 - 113909
Опубликована: Март 1, 2024
The
deciduous
tree
Idesia
polycarpa
can
provide
premium
edible
oil
with
high
polyunsaturated
fatty
acid
contents.
Here,
we
generate
its
high-quality
reference
genome,
which
is
∼1.21
Gb,
comprising
21
pseudochromosomes
and
42,086
protein-coding
genes.
Phylogenetic
genomic
synteny
analyses
show
that
it
diverged
Populus
trichocarpa
about
16.28
million
years
ago.
Notably,
most
biosynthesis
genes
are
not
only
increased
in
number
genome
but
also
highly
expressed
the
fruits.
Moreover,
identify,
through
genome-wide
association
analysis
RNA
sequencing,
I.
SUGAR
TRANSPORTER
5
(IpSTP5)
gene
as
a
positive
regulator
of
accumulation
Silencing
IpSTP5
by
virus-induced
silencing
causes
significant
reduction
content
fruits,
suggesting
has
potential
to
be
used
molecular
marker
breed
high-oil-content
cultivars.
Our
results
collectively
lay
foundation
for
breeding
elite
cultivars
polycarpa.
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Авг. 17, 2024
Biological
invasions
pose
a
significant
threat
to
ecosystems,
disrupting
local
biodiversity
and
ecosystem
functions.
The
genomic
underpinnings
of
invasiveness,
however,
are
still
largely
unknown,
making
it
difficult
predict
manage
invasive
species
effectively.
common
reed
(Phragmites
australis)
is
dominant
grass
in
wetland
ecosystems
has
become
particularly
when
transferred
from
Europe
North
America.
Here,
we
present
high-quality
gap-free,
telomere-to-telomere
genome
assembly
Phragmites
australis
consisting
24
pseudochromosomes
B
chromosome.
Fully
phased
subgenomes
demonstrated
considerable
subgenome
dominance
revealed
the
divergence
diploid
progenitors
approximately
30.9
million
years
ago.
Comparative
genomics
using
chromosome-level
scaffolds
for
three
other
lineages
previously
published
draft
an
lineage
that
gene
family
expansions
form
tandem
duplications
may
have
contributed
invasiveness
lineage.
This
study
sheds
light
on
evolution
Arundinoideae
grasses
suggests
genetic
drivers,
such
as
duplications,
underly
processes
biological
invasion
plants.
These
findings
provide
crucial
step
toward
understanding
managing
basis
plant
species.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 20, 2024
Lycophytes
and
ferns
represent
one
of
the
earliest-diverging
lineages
vascular
plants,
with
Lycopodiaceae
family
constituting
basal
clade
among
lycophytes.
In
this
research,
we
successfully
assembled
annotated
complete
Lycopodium
japonicum
Thunb.
(
L
.
)
mitochondrial
genome
(mitogenome)
utilizing
PacBio
HiFi
sequencing
data,
resulting
in
a
single
circular
molecule
size
454,458
bp.
64
unique
genes
were
altogether,
including
34
protein-coding
genes,
27
tRNAs
3
rRNAs.
It
also
contains
32
group
II
introns,
all
which
undergo
cis
-splicing.
We
identified
195
simple
sequence
repeats,
1,948
dispersed
92
tandem
repeats
mitogenome.
Collinear
analysis
indicated
that
mitogenomes
are
remarkably
conserved
compared
to
those
other
plants.
totally
326
RNA
editing
sites
31
299
converting
cytosine
uracil
reverse.
Notably,
L.
mitogenome
has
small
amounts
foreign
DNA
from
plastid
or
nuclear
origin,
accounting
for
only
2.81%
The
maximum
likelihood
phylogenetic
based
on
23
diverse
land
plant
genomes
supports
position
lycophytes
within
plants
they
form
sister
lineages,
is
consistent
PPG
I
classification
system.
As
first
reported
Lycopodioideae
subfamily,
study
enriches
our
understanding
mitogenomes,
sets
stage
future
research
diversity
evolution
ferns.
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Март 24, 2025
Prunus
subgenus
Cerasus
(Mill)
A.
Gray,
commonly
known
as
cherries
and
cherry
blossoms,
possesses
significant
edible
ornamental
value.
However,
the
mitochondrial
genomes
(mitogenomes)
of
species
remain
largely
unexplored.
Here,
we
successfully
assembled
mitogenomes
five
(
P.
campanulata
,
fruticosa
mahaleb
pseudocerasus
sp
eciosa
),
revealing
common
circular
structures.
The
exhibited
sizes
ranging
from
383,398
bp
to
447,498
bp,
with
GC
content
varying
between
45.54%
45.76%.
A
total
62
69
genes
were
annotated,
variability
in
copy
number
protein-coding
(PCGs)
tRNA
genes.
Mitogenome
collinearity
analysis
indicated
genomic
rearrangements
across
species,
driven
by
repetitive
sequences,
particularly
dispersed
repeats.
Additionally,
displayed
highly
conserved
codon
usage
RNA
editing
patterns,
highlighting
evolutionary
conservation
PCGs.
Phylogenetic
analyses
confirmed
monophyly
subg.
although
notable
phylogenetic
incongruences
observed
plastid
datasets.
These
results
provide
resources
for
forthcoming
studies
on
evolution
molecular
breeding
mitogenomes,
enhancing
overall
comprehension
mitogenome
structure
within
.
Forests,
Год журнала:
2024,
Номер
15(7), С. 1117 - 1117
Опубликована: Июнь 27, 2024
Ilex
rotunda
Thunb.
stands
as
a
representative
tree
species
in
subtropical
evergreen
broad-leaved
forests,
widely
distributed
across
southeast
Asia.
This
holds
significant
value
forestry
due
to
its
ecological
resilience
and
adaptability.
Although
researchers
have
conducted
in-depth
research
on
the
plastid
genome
(plastome)
of
I.
rotunda,
mitochondrial
(mitogenome)
this
has
remained
undocumented.
In
present
study,
we
successfully
sequenced
assembled
mitogenome.
The
mitogenome
circular
structure
is
567,552
bp
total
length,
with
GC
content
45.47%.
composition
encompasses
40
protein-coding
genes,
along
3
rRNA
genes
19
tRNA
genes.
Notably,
exhibits
universal
distribution
repetitive
sequences,
but
length
repeats
contributes
relatively
small
proportion
(4%)
whole
mitogenome,
suggesting
that
do
not
serve
primary
cause
amplification
mitogenomes.
Collinear
analysis
indicates
very
conservative
within
Aquifoliales
species.
Additionally,
our
identified
51
fragments
genomic
DNA,
which
migrated
from
plastome
into
five
remaining
intact.
Eventually,
phylogenetic
analyses
based
plastomes
mitogenomes
36
angiosperms
determine
be
basal
group
campanulids.
study
establishes
bedrock
for
prospective
investigations
molecular
breeding
research.
