Seasonal Dynamics of Kiwifruit Microbiome: A Case Study in a KVDS-Affected Orchard

Microbiological Research, Год журнала: 2025, Номер unknown, С. 128044 - 128044

Опубликована: Янв. 1, 2025

Язык: Английский

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Microbial Dysbiosis Linked to Metabolic Dysfunction-Associated Fatty Liver Disease in Asians: Prevotella copri Promotes Lipopolysaccharide Biosynthesis and Network Instability in the Prevotella Enterotype

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2183 - 2183

Опубликована: Фев. 11, 2024

Metabolic dysfunction-associated fatty liver disease (MAFLD), formerly known as non-alcoholic (NAFLD), is characterized by hepatic fat accumulation metabolic dysfunction. The rising prevalence of MAFLD, especially among Asians, may be associated with changes in gut microbiota. We investigated microbiota characteristics and potential mechanisms leading to MAFLD development according enterotypes. Case-control studies examining the composition between non-MAFLD participants were searched public databases until July 2023. Gut was categorized into two enterotypes principal component analysis. According enterotypes, LEfSe, ALDEx2, XGBoost, DCiPatho utilized identify differential abundances pathogenic microbes groups. analyzed microbial community networks SprCC module predicted functions. In Prevotella enterotype (ET-P), 98.6% Asians 65.1% Caucasians (

Язык: Английский

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Hyperbaric oxygen augments susceptibility to C. difficile infection by impairing gut microbiota ability to stimulate the HIF-1α-IL-22 axis in ILC3

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Янв. 2, 2024

Hyperbaric oxygen (HBO) therapy is a well-established method for improving tissue oxygenation and typically used the treatment of various inflammatory conditions, including infectious diseases. However, its effect on intestinal mucosa, microenvironment known to be physiologically hypoxic, remains unclear. Here, we demonstrated that daily with hyperbaric affects gut microbiome composition, worsening antibiotic-induced dysbiosis. Accordingly, HBO-treated mice were more susceptible Clostridioides difficile infection (CDI), an enteric pathogen highly associated colitis. These observations closely linked decline in level microbiota-derived short-chain fatty acids (SCFAs). Butyrate, SCFA produced primarily by anaerobic microbial species, mitigated HBO-induced susceptibility CDI increased epithelial barrier integrity group 3 innate lymphoid cell (ILC3) responses. Mice displaying tissue-specific deletion HIF-1 RORγt-positive cells exhibited no protective butyrate during CDI. In contrast, reinforcement signaling through conditional VHL disease outcome, even after HBO therapy. Taken together, conclude induces dysbiosis impairs production SCFAs affecting HIF-1α-IL-22 axis ILC3 response subsequent C. infection.

Язык: Английский

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Traffic-related air pollution (TRAP) exposure, lung function, airway inflammation and expiratory microbiota: A randomized crossover study

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 289, С. 117545 - 117545

Опубликована: Янв. 1, 2025

Язык: Английский

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Effects of chronic metal exposure and metamorphosis on the microbiomes of larval and adult insects, and riparian spiders through the aquatic-riparian food web.

Environmental Pollution, Год журнала: 2025, Номер unknown, С. 125867 - 125867

Опубликована: Фев. 1, 2025

Язык: Английский

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Integrative analysis of single-cell RNA-seq and gut microbiome metabarcoding data elucidates macrophage dysfunction in mice with DSS-induced ulcerative colitis

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Июнь 15, 2024

Ulcerative colitis (UC) is a significant inflammatory bowel disease caused by an abnormal immune response to gut microbes. However, there are still gaps in our understanding of how and metabolic changes specifically contribute this disease. Our research aims address gap examining mouse colons after inducing ulcerative colitis-like symptoms. Employing single-cell RNA-seq 16 s rRNA amplicon sequencing analyze distinct cell clusters microbiomes the colon at different time points induction with dextran sodium sulfate. We observe reduction epithelial populations during acute colitis, indicating tissue damage, partial recovery observed chronic inflammation. Analyses cell-cell interactions demonstrate shifts networking patterns among types progression. Notably, macrophage phenotypes exhibit diversity, pronounced polarization towards pro-inflammatory M1 phenotype conditions, suggesting role heterogeneity severity. Increased expression Nampt NOX2 complex subunits UC macrophages contributes processes. The microbiome exhibits reduced taxonomic diversity compared healthy conditions UC. study also highlights T differentiation context dysbiosis its implications progression, emphasizing need for targeted interventions modulate balance colitis.

Язык: Английский

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A bud's life: Metabarcoding analysis to characterise hazelnut big buds microbiome biodiversity

Microbiological Research, Год журнала: 2024, Номер 287, С. 127851 - 127851

Опубликована: Июль 24, 2024

Despite Corylus avellana L. being an economically important shrub species known for its resilience to adverse environmental conditions, it constantly faces attacks from a plethora of biotic entities. Among these, the mite pest Phytoptus avellanae is gaining importance, causing economic losses every year. This colonises new generative and vegetative buds, leading them become swollen reddish, drastically reducing hazelnut production. The biology behind gall formation still poorly understood. study provides qualitative quantitative description microbiome in both healthy infested buds two cultivars through metabarcoding fungal ITS bacterial 16 S. Potentially pathogenic genera such as Fusarium Pseudomonas were predominant along with obligate intracellular genus Wolbachia. Akanthomyces muscarius was instead isolated culture-based methods only buds. These findings could improve understanding ecology, supporting management populations, they also serve milestone further studies on low-impact, monitoring-driven, genetically targeted control strategies.

Язык: Английский

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Ectomycorrhizal fungi alter soil food webs and the functional potential of bacterial communities

mSystems, Год журнала: 2024, Номер 9(6)

Опубликована: Май 8, 2024

Most of Earth's trees rely on critical soil nutrients that ectomycorrhizal fungi (EcMF) liberate and provide, all land plants associate with bacteria help them survive in nature. Yet, our understanding how the presence EcMF modifies bacterial communities, food webs, root chemistry requires direct experimental evidence to comprehend effects may generate belowground plant microbiome. To this end, we grew

Язык: Английский

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Beneficial Effects of Synbiotics on the Gut Microbiome in Individuals with Low Fiber Intake: Secondary Analysis of a Double-Blind, Randomized Controlled Trial

Nutrients, Год журнала: 2024, Номер 16(13), С. 2082 - 2082

Опубликована: Июнь 29, 2024

Insufficient dietary fiber intake can negatively affect the intestinal microbiome and, over time, may result in gut dysbiosis, thus potentially harming overall health. This randomized controlled trial aimed to improve of individuals with low (<25 g/day) during a 7-week synbiotic intervention. The metabolically healthy male participants (n = 117, 32 ± 10 y, BMI 25.66 3.1 kg/m2) were divided into two groups: one receiving supplement (Biotic Junior, MensSana AG, Forchtenberg, Germany) and other placebo, without altering their habits or physical activity. These groups further stratified by group (LFG) high (HFG). Stool samples for analysis collected before after Statistical was performed using linear mixed effects partial least squares models. At baseline, microbiomes LFG HFG partially separated. After seven weeks intervention, abundance SCFA-producing microbes significantly increased LFG, which is known health; however, this effect less pronounced HFG. Beneficial on be achieved synbiotics, demonstrating importance personalized synbiotics.

Язык: Английский

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Organic matter accumulation drives methylotrophic methanogenesis and microbial ecology in a hypersaline coastal lagoon

Limnology and Oceanography, Год журнала: 2024, Номер 69(9), С. 1970 - 1983

Опубликована: Июль 20, 2024

Abstract Hypersalinity is common in coastal wetlands throughout warm, tropical, and arid regions. Climate‐induced changes rainfall, sea level, anthropogenic modification to basins coastlines are likely further increase salinization these ecosystems. Yet, carbon cycling hypersaline not well understood, poorly constrained climate models. In the Coorong, a eutrophic, lagoon, recognized as internationally important under Ramsar convention, organic matter rapidly accumulates deeper areas of through settling fine detrital particles, phytoplankton suspended sediments. During initial surveys, elevated surface water methane (CH 4 ) concentrations were observed above depositional To identify drivers CH production, sediment characteristics assessed Genetic markers (i.e., 16rDNA mcrA functional gene) used characterize microbial communities. With multiple lines evidence, this study identifies matter, methanogen abundance, salinity which concentrated zones. Archaea also more abundant zones, including methylotrophic methanogens: Methanofastidiosales, Methanomasiliicoccales, Methermicoccaceae , Methanococcoides . These methanogens highly correlated porewater, suggesting an influence methanogenesis. investigate further, metabolic genes predicted from 16S rRNA with PICRUSt2. This represents first effort analyze dynamics underscoring need integrate unique ecosystems into global models enhance our understanding greenhouse gas emissions changing climate.

Язык: Английский

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