Anti-SARS-CoV-2 serology based on ancestral RBD antigens does not correlate with the presence of neutralizing antibodies against Omicron variants DOI Creative Commons

Léa Dépéry,

Isabelle Bally, Axelle Amen

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер 13(1)

Опубликована: Ноя. 20, 2024

ABSTRACT Neutralizing antibody titers and binding levels are considered correlates of protection against severe SARS-CoV-2 infection. The clinical utility serology should be reevaluated in light the emergence escape variants, as commercial antibody-binding assays have not been adapted to virus’ antigenic evolution. We compared anti-SARS-CoV-2 four quantitative serological tests based on variable ancestral spike antigens (three in-house ELISAs prototype VIDAS IgG QUANT assay) neutralization pseudotyped Wuhan, BA.2, BA.4/5, BQ.1.1, XBB.1.1 viruses a cohort 100 patients infected 2020 or during Omicron waves. Binding correlated well with neutralizing for but association decreased BQ.1.1 XBB.1 (for assay, Spearman’s correlation was 0.82 [95% CI 0.74–0.88] 0.61 [0.46–0.72] BA.2 XBB.1, respectively). In 15% no antibodies assay still yielded ranging from 74 7,652 units/mL. Using an adjusted threshold receiver operating characteristic (ROC) curve analysis, specificity detection increased 0.15 (95% 0.02–0.45) 0.17 (0.04–0.41) 0.92 (0.64–1.00) 0.83 (0.59–0.96) respectively. Serological receptor-binding domain virus fail predict activity latest circulating variants. Adapting may improve their immunocompromised patients. IMPORTANCE Anti-SARS-CoV-2 developed response COVID-19 pandemic diagnose infection monitor individual’s immunity following natural vaccination. Given relationship between infection, many studies evaluated pre-Omicron era. An important potential use serology, which explores antibodies, is estimating level new particularly immunosuppressed individuals those at risk COVID. However, era, evade induced by previous infections vaccination, determined re-examined order determine whether these optimized adapting strains.

Язык: Английский

Serological cross-reactivity using a SARS-CoV-2 ELISA test in acute Zika virus infection, Colombia DOI Creative Commons
Álvaro A. Faccini‐Martínez, Ricardo Rivero, Evelin Garay

и другие.

International Journal of Infectious Diseases, Год журнала: 2020, Номер 101, С. 191 - 193

Опубликована: Сен. 28, 2020

ObjectivesWe investigated seroreactivity by using a commercial SARS-CoV-2 ELISA test in samples collected from different groups of individuals, including patients diagnosed to have Dengue, Zika, and Chikungunya infection between 2015 2019, an endemic area the Caribbean Colombian region.MethodsA total 127 sera obtained six individuals were included this study: Group A: with confirmed infection; B: symptoms suggestive COVID-19 or asymptomatic contacts patients; C: acute recent dengue virus D: Zika E: previous F: exposure spotted fever group rickettsiae.ResultsOverall, A, B, D showed 92%, 75%, 26% samples, respectively; furthermore, C, E, F 100% seronegativity.ConclusionsWe found serological cross-reactivity test. Further studies are needed evaluate whether cross-reaction is maintained time nonacute its effect serosurveys areas for arbovirus.

Язык: Английский

Процитировано

39
Detection of SARS-CoV-2 IgG antibodies in dried blood spots DOI Open Access
Coleman Turgeon,

Karen A. Sanders,

Dane Granger

и другие.

Diagnostic Microbiology and Infectious Disease, Год журнала: 2021, Номер 101(1), С. 115425 - 115425

Опубликована: Май 13, 2021

Язык: Английский

Процитировано

31
Prevalence of SARS-CoV-2 antibodies among Swiss hospital workers: Results of a prospective cohort study DOI Creative Commons
Philipp Köhler, Christian R. Kahlert, Johannes Sumer

и другие.

Infection Control and Hospital Epidemiology, Год журнала: 2020, Номер 42(5), С. 604 - 608

Опубликована: Окт. 8, 2020

Abstract In this prospective cohort of 1,012 Swiss hospital employees, 3 different assays were used to screen serum for SARS-CoV-2 antibodies. Seropositivity was 1%; the positive predictive values lateral-flow immunoassay 64% (IgG) and 13% (IgM). History fever myalgia most effectively differentiated seropositive seronegative participants.

Язык: Английский

Процитировано

26
Design and Assessment of a Surface Plasmon Resonance Biosensor for Recognition of Dengue Infected Plasma, Platelets, and Hemoglobin Cells DOI

Yousif S. Adam,

Trupti Kamani,

Shobhit K. Patel

и другие.

Plasmonics, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 11, 2024

Язык: Английский

Процитировано

3
4D-DIA Proteomics Uncovers New Insights into Host Salivary Response Following SARS-CoV-2 Omicron Infection DOI Creative Commons
Iasmim Lopes de Lima, Thaís Regiani Cataldi, Carlos Brites

и другие.

Journal of Proteome Research, Год журнала: 2025, Номер 24(2), С. 499 - 514

Опубликована: Янв. 13, 2025

Since late 2021, Omicron variants have dominated the epidemiological scenario as most successful severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sublineages, driving new and breakthrough infections globally over past two years. In this study, we investigated for first time host salivary response of COVID-19 patients infected with (BA.1, BA.2, BA.4/5) by using an untargeted four-dimensional data-independent acquisition (4D-DIA)-based proteomics approach. We identified 137 proteins whose abundance levels differed between positive negative groups. Salivary signatures were mainly enriched in ribosomal proteins, linked to mRNAviral translation, protein synthesis processing, immune innate, antiapoptotic signaling. The higher 14-3-3 (YWHAG, YWHAQ, YWHAE, SFN) saliva, reported here, may be associated increased infectivity improved viral replicative fitness. also seven (ACTN1, H2AC2, GSN, NDKA, CD109, GGH, PCYOX) that yielded comprehension into infection performed outstandingly screening a hospital setting. This panel presented enhanced anti-COVID-19 anti-inflammatory signature, providing insights disease severity, supported comparisons other proteome data sets. signature provided valuable host's SARS-CoV-2 infection, shedding light on pathophysiology COVID-19, particularly cases mild disease. It underscores potential clinical applications saliva settings. Data are available via ProteomeXchange identifier PXD054133.

Язык: Английский

Процитировано

0
SARS-CoV-2 neutralizing antibody determination after vaccination using spectrophotometric measurement of lateral flow immunochromatography DOI Creative Commons
Jianglei Ma,

Scott Kaniper,

Yuliya Vabishchevich

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 24, 2025

Neutralizing antibody titers have been found to be strongly correlated with observed vaccine effectiveness against symptomatic and severe COVID-19. Few non-high complexity assays are currently available detect the presence of neutralizing antibodies. This retrospective single-center cross-sectional study compared performance a lateral flow immunochromatography assay coupled spectrophotometric measurement system for detecting SARS-CoV-2 antibodies an enzyme-linked immunosorbent (ELISA) neutralization in context post-vaccination responses. The limit detection was similar ELISA strong linearity throughout measuring interval. Repeatability, interfering substances, cross-reactivity studies were robust. Results 274 plasma samples on whom RNA test vaccination status, including number manufacturer, known showed positive predictive value (PPV) 99.0% (CI 96.4–99.7%) negative (NPV) 91.9% 83.4–96.2%) ELISA. PPV all manufacturer subgroups > 95% except those individuals who had only 1 Pfizer (PPV 80%). NPV PCR no vaccinations 100% while 88.1% without previous or vaccination. 80% contrast Moderna vaccinations. Alternative methodologies requiring less sophisticated laboratory support measure may useful

Язык: Английский

Процитировано

0
Fresh frozen plasma for neutralizing SARS-CoV-2: “An exploratory cross-sectional study and review of the state of the art” DOI

Marcial Delgado‐Fernández,

Juan Diego Ruíz-Mesa,

Almudena Rojas-González

и другие.

Enfermedades Infecciosas y Microbiología Clínica, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Fresh frozen plasma for neutralizing SARS-CoV-2: “An exploratory cross-sectional study and review of the state of the art” DOI

Marcial Delgado‐Fernández,

Juan Diego Ruíz-Mesa,

Almudena Rojas-González

и другие.

Enfermedades infecciosas y microbiologia clinica (English ed ), Год журнала: 2025, Номер 43(5), С. 282 - 290

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Viral Laboratory Diagnosis DOI
Denver Niles, James J. Dunn

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 2790 - 2805.e2

Опубликована: Янв. 1, 2025

Процитировано

0
Editorial: Multisystem Inflammatory Syndrome in Adults (MIS-A) and the Spectrum of COVID-19 DOI

Dinah V. Parums

Medical Science Monitor, Год журнала: 2021, Номер 27

Опубликована: Окт. 11, 2021

Recent studies on the pathogenesis and clinical spectrum of human disease following infection with new pathogen, SARS-CoV-2, have identified varied presentations sequelae COVID-19. Acute 'cytokine storm' in severe COVID-19 results multiorgan damage due to vascular hyperpermeability, edema, hypercoagulation. The long-term consequences from SARS-CoV-2 include long COVID. or post-COVID syndrome, multisystem inflammatory syndrome children (MIS-C). Several case reports adults (MIS-A) shown presentation at more than four weeks after initial 21 years age. In September 2021, a published systematic review literature 221 patients MIS-A, representing most comprehensive study date. MIS-A occurs post-acute period. may involve dysregulated antibody-mediated immune response, similar MIS-C. Therefore, respond supportive therapies that control hyperinflammation. This Editorial aims describe discuss as hyperinflammatory terms severity, extent, duration, patient

Язык: Английский

Процитировано

20