4D-DIA Proteomics Uncovers New Insights into Host Salivary Response Following SARS-CoV-2 Omicron Infection

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: 24(2), P. 499 - 514

Published: Jan. 13, 2025

Since late 2021, Omicron variants have dominated the epidemiological scenario as most successful severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sublineages, driving new and breakthrough infections globally over past two years. In this study, we investigated for first time host salivary response of COVID-19 patients infected with (BA.1, BA.2, BA.4/5) by using an untargeted four-dimensional data-independent acquisition (4D-DIA)-based proteomics approach. We identified 137 proteins whose abundance levels differed between positive negative groups. Salivary signatures were mainly enriched in ribosomal proteins, linked to mRNAviral translation, protein synthesis processing, immune innate, antiapoptotic signaling. The higher 14-3-3 (YWHAG, YWHAQ, YWHAE, SFN) saliva, reported here, may be associated increased infectivity improved viral replicative fitness. also seven (ACTN1, H2AC2, GSN, NDKA, CD109, GGH, PCYOX) that yielded comprehension into infection performed outstandingly screening a hospital setting. This panel presented enhanced anti-COVID-19 anti-inflammatory signature, providing insights disease severity, supported comparisons other proteome data sets. signature provided valuable host's SARS-CoV-2 infection, shedding light on pathophysiology COVID-19, particularly cases mild disease. It underscores potential clinical applications saliva settings. Data are available via ProteomeXchange identifier PXD054133.

Language: Английский

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Seroprevalence of SARS-CoV-2 IgG antibodies in children seeking medical care in Seattle, WA June 2020 to December 2022

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

ABSTRACT Seroprevalence studies play an important role in estimating the number of children infected with SARS-CoV-2. We report SARS-CoV-2 seroprevalence seeking medical care for any reason at a free-standing pediatric hospital Seattle, WA over 2.5-year period and four distinct pandemic waves. randomly selected residual serum samples from young adults as inpatients outpatients Seattle Children’s Hospital between June 2020 December 2022 to test presence anti-nucleocapsid (N) antibodies. Samples were categorized into waves based on Washington State epidemiology: Wave 1 (June 2020–October 2020), 2 (November 2020–June 2021), 3 (July 2021–November 4 (December 2021–December 2022). Patient characteristics COVID-19 vaccine status obtained, zip codes used ascertain Social Vulnerability Index (SVI). Multivariable Poisson regression models robust variance estimates examine relationship patient anti-N-positivity each wave. Among 8,040 7,102 patients included analyses, rose 2.4% (95% CI, 2.0%–3.1%) 25.5% CI 23.3%–27.8%) (following Omicron surge). High SVI, Hispanic ethnicity, or use government insurance was associated increased anti-N positivity most observed steady increase followed by sharp after surge early 2022. Our data demonstrate burden specific groups health disparities within our region throughout pandemic. IMPORTANCE results highlight importance seropositivity essential tools provide information incidence prevalence seropositivity. also reinforce other reports demonstrating inequitable that this continued persist pandemic, even high adherence mitigation efforts. It highlights SVI’s value identifying communities must be part research, public vaccination strategies.

Language: Английский

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Fresh frozen plasma for neutralizing SARS-CoV-2: “An exploratory cross-sectional study and review of the state of the art”

Enfermedades Infecciosas y Microbiología Clínica, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Design and Assessment of a Surface Plasmon Resonance Biosensor for Recognition of Dengue Infected Plasma, Platelets, and Hemoglobin Cells

Plasmonics, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Language: Английский

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ORCHESTRA Delphi Consensus: Diagnostic and Therapeutic Management of SARS-CoV-2 Infection in Solid Organ Transplant Recipients

Clinical Microbiology and Infection, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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SARS-CoV-2 neutralizing antibody determination after vaccination using spectrophotometric measurement of lateral flow immunochromatography

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 24, 2025

Neutralizing antibody titers have been found to be strongly correlated with observed vaccine effectiveness against symptomatic and severe COVID-19. Few non-high complexity assays are currently available detect the presence of neutralizing antibodies. This retrospective single-center cross-sectional study compared performance a lateral flow immunochromatography assay coupled spectrophotometric measurement system for detecting SARS-CoV-2 antibodies an enzyme-linked immunosorbent (ELISA) neutralization in context post-vaccination responses. The limit detection was similar ELISA strong linearity throughout measuring interval. Repeatability, interfering substances, cross-reactivity studies were robust. Results 274 plasma samples on whom RNA test vaccination status, including number manufacturer, known showed positive predictive value (PPV) 99.0% (CI 96.4–99.7%) negative (NPV) 91.9% 83.4–96.2%) ELISA. PPV all manufacturer subgroups > 95% except those individuals who had only 1 Pfizer (PPV 80%). NPV PCR no vaccinations 100% while 88.1% without previous or vaccination. 80% contrast Moderna vaccinations. Alternative methodologies requiring less sophisticated laboratory support measure may useful

Language: Английский

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Fresh frozen plasma for neutralizing SARS-CoV-2: “An exploratory cross-sectional study and review of the state of the art”

Enfermedades infecciosas y microbiologia clinica (English ed ), Journal Year: 2025, Volume and Issue: 43(5), P. 282 - 290

Published: May 1, 2025

Language: Английский

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Prevalence of SARS-CoV-2 antibodies among Swiss hospital workers: Results of a prospective cohort study

Infection Control and Hospital Epidemiology, Journal Year: 2020, Volume and Issue: 42(5), P. 604 - 608

Published: Oct. 8, 2020

Abstract In this prospective cohort of 1,012 Swiss hospital employees, 3 different assays were used to screen serum for SARS-CoV-2 antibodies. Seropositivity was 1%; the positive predictive values lateral-flow immunoassay 64% (IgG) and 13% (IgM). History fever myalgia most effectively differentiated seropositive seronegative participants.

Language: Английский

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Editorial: Multisystem Inflammatory Syndrome in Adults (MIS-A) and the Spectrum of COVID-19

Medical Science Monitor, Journal Year: 2021, Volume and Issue: 27

Published: Oct. 11, 2021

Recent studies on the pathogenesis and clinical spectrum of human disease following infection with new pathogen, SARS-CoV-2, have identified varied presentations sequelae COVID-19. Acute 'cytokine storm' in severe COVID-19 results multiorgan damage due to vascular hyperpermeability, edema, hypercoagulation. The long-term consequences from SARS-CoV-2 include long COVID. or post-COVID syndrome, multisystem inflammatory syndrome children (MIS-C). Several case reports adults (MIS-A) shown presentation at more than four weeks after initial 21 years age. In September 2021, a published systematic review literature 221 patients MIS-A, representing most comprehensive study date. MIS-A occurs post-acute period. may involve dysregulated antibody-mediated immune response, similar MIS-C. Therefore, respond supportive therapies that control hyperinflammation. This Editorial aims describe discuss as hyperinflammatory terms severity, extent, duration, patient

Language: Английский

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Assessing the association between antibody status and symptoms of long COVID: A multisite study

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(6), P. e0304262 - e0304262

Published: June 6, 2024

The association between SARS-CoV-2 humoral immunity and post-acute sequelae of COVID-19 (long COVID) remains uncertain. objective this population-based cohort study was to assess the seropositivity symptoms consistent with long COVID. English Spanish-speaking members ≥ 18 years old serologic testing conducted prior August 2021 were recruited from Kaiser Permanente Southern California Colorado. Between November April 2022, participants completed a survey assessing symptoms, physical health, mental cognitive function Survey results linked antibody (Ab) viral (RNA) lab in electronic health records. Weighted descriptive analyses generated for five mutually exclusive patient groups: (1) +Ab/+RNA; (2) +Ab/- or missing RNA; (3) -Ab/+RNA; (4a) -Ab/-RNA reporting no infection; (4b) infection. proportions +Ab/+RNA -Ab/+RNA groups compared, adjusted covariates. Among 3,946 participants, mean age 52.1 (SD 15.6), 68.3% female, 28.4% Hispanic, occurred median 15 months (IQR = 12–18). Three quarters (74.5%) reported having had COVID-19. people laboratory-confirmed COVID-19, there positivity (+Ab/+RNA vs. -Ab/+RNA) any function. As expected, function, fatigue worse, palpitations headaches limiting ability work more prevalent among infection positive serology (+Ab/+RNA) compared those without confirmed negative (-Ab/-RNA/no COVID-19). practice settings not associated COVID status suggesting limited utility

Language: Английский

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