The ecological roles of bacterial chemotaxis

Nature Reviews Microbiology, Год журнала: 2022, Номер 20(8), С. 491 - 504

Опубликована: Март 15, 2022

Язык: Английский

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The spread of antibiotic resistance to humans and potential protection strategies

Ecotoxicology and Environmental Safety, Год журнала: 2023, Номер 254, С. 114734 - 114734

Опубликована: Март 10, 2023

Antibiotic resistance is currently one of the greatest threats to human health. Widespread use and residues antibiotics in humans, animals, environment can exert selective pressure on antibiotic bacteria (ARB) gene (ARG), accelerating flow resistance. As ARG spreads population, burden humans increases, which may have potential health effects people. Therefore, it critical mitigate spread reduce load humans. This review briefly described information global consumption national action plans (NAPs) combat provided a set feasible control strategies for transmission ARB three areas including (a) Reducing colonization capacity exogenous ARB, (b) Enhancing mitigating horizontal transfer (HGT) ARG, (c) Reversing With hope achieving interdisciplinary one-health prevention bacterial

Язык: Английский

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Plant Growth-Promoting Bacteria (PGPB) with Biofilm-Forming Ability: A Multifaceted Agent for Sustainable Agriculture

Diversity, Год журнала: 2023, Номер 15(1), С. 112 - 112

Опубликована: Янв. 13, 2023

Plant growth-promoting bacteria (PGPB) enhance plant growth, as well protect plants from several biotic and abiotic stresses through a variety of mechanisms. Therefore, the exploitation PGPB in agriculture is feasible it offers sustainable eco-friendly approaches to maintaining soil health while increasing crop productivity. The vital key application its effectiveness colonizing roots phyllosphere, developing protective umbrella formation microcolonies biofilms. Biofilms offer benefits PGPB, such enhancing resistance adverse environmental conditions, protecting against pathogens, improving acquisition nutrients released environment, facilitating beneficial bacteria–plant interactions. bacterial biofilms can successfully compete with other microorganisms found on surfaces. In addition, plant-associated are capable colonization sites, cycling nutrients, pathogen defenses, tolerance stresses, thereby agricultural productivity yields. This review highlights role surfaces strategies used by biofilm-forming PGPB. Moreover, factors influencing biofilm at root shoot interfaces critically discussed. will pave formulations addressing challenges related their efficacy competence for sustainability.

Язык: Английский

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Coping with stress: How bacteria fine-tune protein synthesis and protein transport

Journal of Biological Chemistry, Год журнала: 2023, Номер 299(9), С. 105163 - 105163

Опубликована: Авг. 14, 2023

Maintaining a functional proteome under different environmental conditions is challenging for every organism, in particular unicellular organisms, such as bacteria. In order to cope with changing environments and stress conditions, bacteria depend on strictly coordinated proteostasis networks that control protein production, folding, trafficking, degradation. Regulation of ribosome biogenesis synthesis are cornerstones this cellular adaptation all domains life, which rationalized by the high energy demand both processes increased resistance translationally silent cells against internal or external poisons. Reduced ultimately also reduces substrate load transport systems, required maintaining periplasmic, inner, outer membrane subproteomes. Consequences impaired have been analyzed several studies generally induce multifaceted response includes upregulation chaperones proteases simultaneous downregulation synthesis. contrast, less known how adjust targeting machineries reduced synthesis, e.g., when encounter face nutrient deprivation. current review, mainly focused using Escherichia coli model we summarize basic concepts activity regulated conditions. addition, highlight some recent developments directly impair bacterial membrane. Finally, describe mechanisms allow maintain stress-responsive proteins canonical pathways impaired. The dynamic regulation balanced compartmentalized essential organism depends network molecular aspects life cycle proteins. This at ribosome, co- posttranslational transport, finally degradation (1Mogk A. Huber D. Bukau B. Integrating homeostasis strategies prokaryotes.Cold Spring Harb. Perspect. Biol. 2011; 3a004366Crossref PubMed Scopus (75) Google Scholar, 2Powers E.T. Powers D.L. Gierasch L.M. 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Choi Weisshaar J.C. spatial biology rapidly growing coli.Front. 2015; 6: 636Crossref (67) 16Beck H.J. Moll Leaderless mRNAs spotlight: ancient but not outdated.Microbiol. Spectr. https://doi.org/10.1128/microbiolspec.RWR-0016-2017Crossref (29) 17Cheng-Guang Gualerzi C.O. switchboard response.Front. 2020; 11619038PubMed 18Vinogradova D.S. Zegarra Maksimova Nakamoto J.A. Kasatsky Paleskava al.How initiating copes ppGpp translate mRNAs.PLoS 18e3000593Crossref (30) 19Trösch Willmund conserved theme hibernation: from chloroplasts plants.Biol. 400: 879-893Crossref 20Wang Liang Zheng Liu An Xu al.Ribosome hibernation bacteria.Protein Pept. Lett. 27: 1082-1091Crossref complemented production proteases, support correct suboptimal degrade aggregated damaged (3Bittner 21Mahmoud S.A. Chien Regulated bacteria.Annu. Biochem. 87: 677-696Crossref (87) 22Bukau Weissman Horwich Molecular quality control.Cell. 2006; 125: 443-451Abstract (1233) A similar initiated membranes saturated due limiting number channels (6Kudva 23Baars Wagner Wickstrom Klepsch Ytterberg A.J. van Wijk K.J. al.Effects SecE depletion proteomes coli.J. Bacteriol. 2008; 190: 3505-3525Crossref (39) 24Wickstrom Simonsson Pop O. Baars al.Characterization consequences YidC 2D blue native/SDS-PAGE.J. 409: 124-135Crossref (34) 25Wang Dalbey R.E. Global change gene expression cell physiology YidC-depleted 2010; 192: 2193-2209Crossref (42) 26Price C.E. Otto Fusetti Becher Hecker Driessen Differential effect aerobic anaerobic growth conditions.Proteomics. 3235-3247Crossref (31) Enhanced cytosolic aggregation cannot be transported induces (26Price 27Bernstein H.D. Hyndman J.B. Physiological basis conservation signal recognition particle pathway 2001; 183: 2187-2197Crossref (53) 28Qi H.Y. 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Overproduction fumarate reductase lipid-protein organelle.J. 1984; 158: 590-596Crossref downregulated ribosomal sequestered inactive aggregates (35Bürk Weiche Wenk Boy Nestel Heimrich al.Depletion inactivates 191: 7017-7026Crossref (20) 36Wickstrom al.Consequences 4598-4609Abstract (32) 37Wagner Klussmeier C.S. Nord overexpression coli.Mol. Cell. Proteomics. 2007; 1527-1550Abstract (269) induced via RNA polymerase (RNAP) RpoH (σ32) (38Oswald Njenga Natriashvili Sarmah SecYEG translocon.Front. Biosci. 8664241Crossref 39Guo M.S. Gross C.A. Stress-induced remodeling proteome.Curr. 24: R424-R434Abstract For executing its function, σ32 needs out-compete housekeeping σ70 subunit, requires presence hyperphosphorylated guanine nucleotides pppGpp, collectively called alarmones (40Jishage Kvint Shingler Nyström sigma factor competition alarmone ppGpp.Genes 16: 1260-1270Crossref (267) 41Steinchen ppGpp: magic modulators metabolism.Front. 11: 2072Crossref Nucleotide second messengers (p)ppGpp act critical signaling molecules during redirect metabolism toward factors, while inhibiting (rRNA) 42Leiva L.E. Ibba At crossroad nucleotide dynamics bacteria.Microbiol. 87e0004422Crossref (1) 43Gottesman Trouble coming: general responses bacteria.J. 294: 11685-11700Abstract (111) Considering approximately one-third entire 44Milo What total per volume? call rethink published values.Bioessays. 35: 1050-1055Crossref (357) 45Li G.W. Burkhardt J.S. Quantifying absolute rates reveals principles underlying allocation resources.Cell. 157: 624-635Abstract (845) execute function outside cytosol, preventing On other hand, it largely unknown activities adapted enter stationary phase starvation. Intriguingly, study has demonstrated accumulation only shuts down (SRP)-dependent (46Czech Mais C.N. Kratzat Giammarinaro Freibert al.Inhibition SRP-dependent secretion (p)ppGpp.Nat. Commun. 2022; 13: 1069Crossref (9) first part use they engage silencing ribosomes. briefly highlights Sec-dependent then describes machinery responds enables coordinate single can contain up 70,000 ribosomes, account 50% content 85% Adjusting turnover available nutrients status therefore critically important survival. reflected rapid increase exponential decline transition into (47Failmezger Ludwig Nieß Siemann-Herzberg fluorescence.FEMS 364: fnx055Crossref (10) (Table primarily determined rate rRNA (42Leiva 48Nomura Saccharomyces cerevisiae: diversity common principles.J. 1999; 181: 6857-6864Crossref Scholar), linked levels (49Deng Beahm D.R. Ionov Sarpeshkar Measuring modeling power consumption living microbial synthetic reporter.BMC 19: 101Crossref (26) 50Schneider D.A. Gaal Gourse R.L. NTP-sensing promoters direct.Proc. Natl. Acad. Sci. U. 99: 8602-8607Crossref (86) contains seven operons, each encoding 16S rRNA, 23S 5S variable tRNA genes (51Maeda Shimada Ishihama Strength coli.PLoS One. 10e0144697Crossref Each operon transcribed two promotors; upstream P1 promoter responsible high-level downstream P2 accounts basal low 2). Transcription portion global transcriptome (52Dennis P.P. Ehrenberg Bremer Control coli: systems approach.Microbiol. 2004; 68: 639-668Crossref (136) Stable binding RNAP promotor operons millimolar concentrations thus diminished drop 2).Table 1Abundance ribosome-hibernation coliProteinProtein abundance (proteins/cell)Ribosome profiling dataaE. MG1655 were grown (A600 = 0.3) Mops medium 2% glucose (347). (45Li Scholar)Protein mass spectrometry dataIBAQIBAQExponential phasebE. BW25113 M9 + 5 g/l glucose. (346Schmidt Kochanowski Vedelaar Ahrné Volkmer Callipo al.The quantitative condition-dependent proteome.Nat. Biotechnol. 34: 104-110Crossref (453) Scholar)Stationary phasecE. (24 h) Scholar)Ribosomal uS2 (RpsB)23,48713,9572432 uL4 (RplD)18,68811,4781409Hibernation RMF16,1221383538 HPF291723124011 RaiA5010304511,711 Sra15,79218355851 RsfA/RsfS796694 YqjD10,88621466209 ElaB11,47723984052 YgaM4734762793 EttA2080n.d.n.d.In general, based data most cases higher than spectrometry. potentially fact does consider degradation, particularly these experiments performed identical strains.Abbreviations: IBAQ, intensity-based quantification; n.d., determined/not detected.a (347Neidhardt F.C. Bloch P.L. Smith D.F. Culture enterobacteria.J. 1974; 119: 736-747Crossref Scholar).b glucose.c Open table tab strains. Abbreviations: detected. Transition further accompanied an polyphosphorylated pppGpp termed stringent 53Bange Brodersen D.E. Liuzzi Steinchen Two P P: understanding (p)ppGpp.Annu. 75: 383-406Crossref (16) 54Bennison D.J. Irving S.E. Corrigan R.M. impact TRAFAC GTPases prokaryotic assembly.Cells. 8: 1313Crossref 55Ferullo Lovett S.T. arrest 4e1000300Crossref (103) 56Irving Choudhury N.R. physiological roles (pp)pGpp bacteria.Nat. 256-271Crossref (119) 57Potrykus Cashel (p)ppGpp: still magical?.Annu. 62: 35-51Crossref (890) 58Hauryliuk Atkinson G.C. Murakami K.S. Tenson Recent insights physiology.Nat. 298-309Crossref controlled GDP/GTP pyrophosphokinase RelA bifunctional synthetase/hydrolase SpoT (41Steinchen 59Pausch Abdelshahid Schäfer Gratani F.L. al.Structural opposing synthetase hydrolase within orchestrator Rel.Cell 32108157Abstract (28) 3A). show very amino acid sequence likely evolved duplication ancestral Rel (60Mittenhuber Comparative genomics evolution synthetases/hydrolases (the Rel, proteins).J. 3: 585-600PubMed domain (53Bange Under nonstress acquires synthetase-off conformation, N-terminal catalytic inhibited C-terminal regulatory domain. However, starvation, interacts uncharged A-site releases 61Brown Fernández I.S. Gordiyenko Ramakrishnan Ribosome-dependent activation control.Nature. 534: 277-280Crossref (144) 3, B C). Among many itself because affinity dissociation ribosome. turn allows reactivation next stalled "hopping model" postulates provides interact tRNAs absence possible binds transfers vacant (62Winther Roghanian Activation loading RelA-tRNA A-site.Mol. 70: 95-105.e4Abstract (62) 63Kushwaha G.S. Bhavesh N.S. Interaction provide evidence prerequisite complex binding.Curr. 65: 1173-1184Crossref (13) Nevertheless, contact appears (64Li Bouveret Wang J.D. starvation diffusive behavior live 571-585Crossref synthesized transferring pyrophosphate 3′-hydroxyl group GDP GTP, respectively. T

Язык: Английский

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Spatial structure, chemotaxis and quorum sensing shape bacterial biomass accumulation in complex porous media

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 2, 2024

Abstract Biological tissues, sediments, or engineered systems are spatially structured media with a tortuous and porous structure that host the flow of fluids. Such complex environments can influence spatial temporal colonization patterns bacteria by controlling transport individual bacterial cells, availability resources, distribution chemical signals for communication. Yet, due to multi-scale these systems, it is hard assess how different biotic abiotic properties work together control accumulation biomass. Here, we explore flow-mediated interactions allow gut commensal Escherichia coli colonize composed heterogenous dead-end pores (DEPs) connecting percolating channels, i.e. transmitting (TPs), mimicking surface mammalian guts. We find in presence flow, gradients quorum sensing (QS) signaling molecule autoinducer-2 (AI-2) promote E. chemotactic DEPs. In this crowded environment, combination growth cell-to-cell collision favors development suspended aggregates. This results hot-spots resource consumption, which, upon limitation, triggers mechanical evasion biomass from nutrients oxygen depleted Our findings demonstrate microscale medium coupled chemotaxis such as villi crypts within soil filters.

Язык: Английский

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Addressing the global challenge of bacterial drug resistance: insights, strategies, and future directions

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 24, 2025

The COVID-19 pandemic underscored bacterial resistance as a critical global health issue, exacerbated by the increased use of antibiotics during crisis. Notwithstanding pandemic's prevalence, initiatives to address medication have been inadequate. Although an overall drop in worldwide antibiotic consumption, total usage remains substantial, requiring rigorous regulatory measures and preventive activities mitigate emergence resistance. National Action Plans (NAPs) implemented worldwide, significant disparities persist, particularly low- middle-income countries (LMICs). Settings such farms, hospitals, wastewater treatment facilities, agricultural environments include presence Antibiotic Resistant Bacteria (ARB) antibiotic-resistance genes (ARG), promoting propagation Dietary modifications probiotic supplementation shown potential reshaping gut microbiota reducing gene prevalence. Combining with adjuvants or bacteriophages may enhance efficacy development. Novel therapeutic approaches, tailored antibiotics, monoclonal antibodies, vaccines, nanoparticles, offer alternate ways addressing In spite advancements next-generation sequencing analytics, gaps persist comprehending role regulating Effectively tackling requires robust policy interventions targeting root causes while minimizing public risks. This review provides information for developing strategies protocols prevent colonization, microbiome resilience, spread

Язык: Английский

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Plant Colonization by Biocontrol Bacteria and Improved Plant Health: A Review

Frontiers in Bioscience-Landmark, Год журнала: 2025, Номер 30(1)

Опубликована: Янв. 14, 2025

The use of biological control agents is one the best strategies available to combat plant diseases in an ecofriendly manner. Biocontrol bacteria capable providing beneficial effect crop growth and health, have been developed for several decades. It highlights need a deeper understanding colonization mechanisms employed by biocontrol enhance their efficacy pathogen control. present review deals with in-depth steps involved host bacteria. process starts from root zone, where establish initial interactions plant’s system. Moving beyond roots, migrate colonize other organs, including stems, leaves, even flowers. Also, attempts explore facilitating bacterial movement within such as migrating through interconnected spaces vessels or apoplast, applying quorum sensing extracellular enzymes during what needed long-term association plant. impacts on microbial community dynamics, nutrient cycling, overall health are discussed, emphasizing intricate relationships between microbiome benefits above-ground parts, 40 confer. By unraveling these mechanisms, researchers can develop targeted enhancing efficiency effectiveness bacteria, leading more sustainability resilience.

Язык: Английский

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An atlas of metabolites driving chemotaxis in prokaryotes

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 1, 2025

Язык: Английский

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Biofilm-mediated bioremediation of xenobiotics and heavy metals: a comprehensive review of microbial ecology, molecular mechanisms, and emerging biotechnological applications

3 Biotech, Год журнала: 2025, Номер 15(4)

Опубликована: Март 7, 2025

Язык: Английский

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Bacterial Motility and Its Role in Skin and Wound Infections

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(2), С. 1707 - 1707

Опубликована: Янв. 15, 2023

Skin and wound infections are serious medical problems, the diversity of bacteria makes such difficult to treat. Bacteria possess many virulence factors, among which motility plays a key role in skin infections. This feature allows for movement over surface relocation into wound. The aim this paper is review type bacterial indicate underlying mechanisms than can serve as target developing or modifying antibacterial therapies applied infection treatment. Five types distinguished: appendage-dependent (swimming, swarming, twitching) appendage-independent (gliding sliding). All them allow relocate aid during infection. Swimming spread from ‘persister cells’ biofilm microcolonies colonise other tissues. Twitching enables press through tissues infection, whereas sliding cocci (defined non-motile) migrate surfaces. swarming display greater resistance antimicrobials. Molecular motors generating focal adhesion complexes cell leaflet generate ‘wave’, pushes cells lacking appendages, thereby enabling movement. Here, we present five main motility, their molecular mechanisms, examples that utilise them. Bacterial migration be considered not only factor but also therapy.

Язык: Английский

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