EMBO Molecular Medicine, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Microbiology, Год журнала: 2023, Номер 169(3)
Опубликована: Март 22, 2023
Type 4 filaments (T4F) are a superfamily of filamentous nanomachines – virtually ubiquitous in prokaryotes and functionally versatile which type pili (T4P) the defining member. T4F polymers pilins, assembled by conserved multi-protein machineries. They have long been an important topic for research because they key virulence factors numerous bacterial pathogens. Our poor understanding molecular mechanisms assembly is serious hindrance to design anti-T4F therapeutics. This review attempts shed light on fundamental mechanistic principles at play focusing similarities rather than differences between several (mostly bacterial) T4F. holistic approach, complemented revolutionary ability artificial intelligence predict protein structures, led intriguing model assembly.
Язык: Английский
Процитировано
27
Biochemical Journal, Год журнала: 2024, Номер 481(4), С. 245 - 263
Опубликована: Фев. 15, 2024
Numerous bacteria naturally occur within spatially organised, multicellular communities called biofilms. Moreover, most bacterial infections proceed with biofilm formation, posing major challenges to human health. Within biofilms, cells are embedded in a primarily self-produced extracellular matrix, which is defining feature of all The matrix complex, viscous mixture composed polymeric substances such as polysaccharides, filamentous protein fibres, and DNA. structured arrangement the bestows beneficial emergent properties that not displayed by planktonic cells, conferring protection against physical chemical stresses, including antibiotic treatment. However, lack multi-scale information at molecular level has prevented better understanding this its properties. Here, we review recent progress on characterisation components their three-dimensional spatial organisation
Язык: Английский
Процитировано
14
Current Opinion in Microbiology, Год журнала: 2024, Номер 79, С. 102468 - 102468
Опубликована: Апрель 4, 2024
Bacteria utilize type IV pili (T4P) to interact with their environment, where they facilitate processes including motility, adherence, and DNA uptake. T4P require multisubunit, membrane-spanning nanomachines for assembly. The tight adherence (Tad) are an Archaea-derived subgroup whose machinery exhibits significant mechanistic architectural differences from bacterial IVa IVb pili. Most Tad biosynthetic genes encoded in a single locus that is widespread bacteria due facile acquisition via horizontal gene transfer. These loci experience extensive structural rearrangements, the of novel regulatory or genes, which fine-tune function. This has permitted integration into many different lifestyles, Caulobacter crescentus cell cycle, Myxococcus xanthus predation, numerous plant mammalian pathogens symbionts.
Язык: Английский
Процитировано
12
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Фев. 1, 2024
Abstract Bacteriophage therapy is one potential strategy to treat antimicrobial resistant or persistent bacterial infections, and the year 2021 marked centennial of Felix d’Hérelle’s first publication on clinical applications phages. At Center for Phage Biology & Therapy at Yale University, a preparatory modular approach has been established offer safe potent phages single-patient investigational new drug while recognizing time constraints imposed by infection(s). This study provides practical walkthrough pipeline with an Autographiviridae phage targeting Pseudomonas aeruginosa (phage vB_PaeA_SB, abbreviated ΦSB). Notably, thorough characterization evolutionary selection pressure exerted bacteria phages, analogous antibiotics, are incorporated into pipeline.
Язык: Английский
Процитировано
10
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Окт. 30, 2024
Язык: Английский
Процитировано
8
Microorganisms, Год журнала: 2024, Номер 12(11), С. 2271 - 2271
Опубликована: Ноя. 8, 2024
The phylum
Язык: Английский
Процитировано
7
The Science of The Total Environment, Год журнала: 2023, Номер 867, С. 161164 - 161164
Опубликована: Янв. 9, 2023
Язык: Английский
Процитировано
15
Nucleic Acids Research, Год журнала: 2023, Номер 51(11), С. 5661 - 5677
Опубликована: Май 13, 2023
Abstract Acinetobacter baumannii is a dangerous nosocomial pathogen, especially due to its ability rapidly acquire new genetic traits, including antibiotic resistance genes (ARG). In A. baumannii, natural competence for transformation, one of the primary modes horizontal gene transfer (HGT), thought contribute ARG acquisition and has therefore been intensively studied. However, knowledge regarding potential role epigenetic DNA modification(s) on this process remains lacking. Here, we demonstrate that methylome pattern diverse strains differs substantially these marks influence fate transforming DNA. Specifically, describe methylome-dependent phenomenon impacts intra- inter-species exchange by competent strain A118. We go identify characterize an A118-specific restriction-modification (RM) system impairs transformation when incoming lacks specific methylation signature. Collectively, our work contributes towards more holistic understanding HGT in organism may also aid future endeavors tackling spread novel ARGs. particular, results suggest exchanges between bacteria share similar epigenomes are favored could guide research into identifying reservoir(s) traits multi-drug resistant pathogen.
Язык: Английский
Процитировано
15
Nature Communications, Год журнала: 2023, Номер 14(1)
Опубликована: Сен. 13, 2023
Abstract The bacterial T ight ad herence S ecretion ystem (TadSS) assembles surface pili that drive cell adherence, biofilm formation and predation. structure mechanism of the TadSS is mostly unknown. This includes characterisation outer membrane secretin through which pilus channelled recruitment its pilotin. Here we investigate RcpA TadD lipoprotein from Pseudomonas aeruginosa . Light microscopy reveals colocalising with in P. when heterologously expressed Escherichia coli We use cryogenic electron to determine how assemble a channel C13 C14 symmetries. Despite low sequence homology, show shares similar fold type 4 system pilotin PilF. establish C-terminal four residues bind - an interaction essential for formation. binding between appears distinct known secretin-pilotin pairings other secretion systems.
Язык: Английский
Процитировано
13
Microbiology, Год журнала: 2023, Номер 169(7)
Опубликована: Июль 26, 2023
Myxobacteria are social microbial predators that use cell–cell contacts to identify bacterial or fungal prey and differentiate kin relatives initiate cellular responses. For killing, they assemble Tad-like type III-like secretion systems at contact sites. discrimination (KD), outer membrane exchange complexes composed of the TraA TraB receptors A VI system Rhs proteins also mediate KD. Following recognition, these deliver appropriate effectors into target cells. prey, this leads cell death lysis for nutrient consumption by myxobacteria. In KD, a panel delivered, if adjacent cells clonal cells, resistance ensues because express cognate immunity factors; while nonkin lack complete intoxicated. This review compares contrasts recent findings from in
Язык: Английский
Процитировано
12