Contributions of F‐specific subunits to the F plasmid‐encoded type IV secretion system and F pilus DOI Open Access
Kouhei Kishida, Rachel E. Bosserman, Laith Harb

et al.

Molecular Microbiology, Journal Year: 2022, Volume and Issue: 117(5), P. 1275 - 1290

Published: April 18, 2022

F plasmids circulate widely among the Enterobacteriaceae through encoded type IV secretion systems (T4SSF s). Assembly of T4SSF s and associated pili requires 10 VirB/VirD4-like Tra subunits eight or more F-specific subunits. Recently, we presented evidence using in situ cryoelectron tomography (cryoET) that undergo structural transitions when activated for pilus production, assembled are deposited onto alternative basal platforms at cell surface. Here, deleted conserved genes from MOBF12C plasmid pED208 quantitated effects on transfer, production by fluorescence microscopy, elaboration structures cryoET. Mutant phenotypes supported assignment into three functional Classes: (i) TraF, TraH, TraW required all -associated activities, (ii) TraU, TraN, TrbC nonessential but contribute significantly to distinct functions, (iii) TrbB is essential not transfer. Equivalent mutations a phylogenetically distantly related MOB12A conferred similar generally these Class assignments. We present new structure-driven model which steps assembly activation regulate DNA transfer dynamics deposition platforms.

Language: Английский

Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) DOI
Vladimir Pelicic

Microbiology, Journal Year: 2023, Volume and Issue: 169(3)

Published: March 22, 2023

Type 4 filaments (T4F) are a superfamily of filamentous nanomachines – virtually ubiquitous in prokaryotes and functionally versatile which type pili (T4P) the defining member. T4F polymers pilins, assembled by conserved multi-protein machineries. They have long been an important topic for research because they key virulence factors numerous bacterial pathogens. Our poor understanding molecular mechanisms assembly is serious hindrance to design anti-T4F therapeutics. This review attempts shed light on fundamental mechanistic principles at play focusing similarities rather than differences between several (mostly bacterial) T4F. holistic approach, complemented revolutionary ability artificial intelligence predict protein structures, led intriguing model assembly.

Language: Английский

Citations

24
The role of filamentous matrix molecules in shaping the architecture and emergent properties of bacterial biofilms DOI Creative Commons
Jan Böhning, Abul K. Tarafder, Tanmay A. M. Bharat

et al.

Biochemical Journal, Journal Year: 2024, Volume and Issue: 481(4), P. 245 - 263

Published: Feb. 15, 2024

Numerous bacteria naturally occur within spatially organised, multicellular communities called biofilms. Moreover, most bacterial infections proceed with biofilm formation, posing major challenges to human health. Within biofilms, cells are embedded in a primarily self-produced extracellular matrix, which is defining feature of all The matrix complex, viscous mixture composed polymeric substances such as polysaccharides, filamentous protein fibres, and DNA. structured arrangement the bestows beneficial emergent properties that not displayed by planktonic cells, conferring protection against physical chemical stresses, including antibiotic treatment. However, lack multi-scale information at molecular level has prevented better understanding this its properties. Here, we review recent progress on characterisation components their three-dimensional spatial organisation

Language: Английский

Citations

14
Optimized preparation pipeline for emergency phage therapy against Pseudomonas aeruginosa at Yale University DOI Creative Commons
Silvia Würstle, Alina Lee, Kaitlyn E. Kortright

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 1, 2024

Abstract Bacteriophage therapy is one potential strategy to treat antimicrobial resistant or persistent bacterial infections, and the year 2021 marked centennial of Felix d’Hérelle’s first publication on clinical applications phages. At Center for Phage Biology & Therapy at Yale University, a preparatory modular approach has been established offer safe potent phages single-patient investigational new drug while recognizing time constraints imposed by infection(s). This study provides practical walkthrough pipeline with an Autographiviridae phage targeting Pseudomonas aeruginosa (phage vB_PaeA_SB, abbreviated ΦSB). Notably, thorough characterization evolutionary selection pressure exerted bacteria phages, analogous antibiotics, are incorporated into pipeline.

Language: Английский

Citations

10
The type IVc pilus: just a Tad different DOI Creative Commons
Gregory B. Whitfield, Yves V. Brun

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 79, P. 102468 - 102468

Published: April 4, 2024

Bacteria utilize type IV pili (T4P) to interact with their environment, where they facilitate processes including motility, adherence, and DNA uptake. T4P require multisubunit, membrane-spanning nanomachines for assembly. The tight adherence (Tad) are an Archaea-derived subgroup whose machinery exhibits significant mechanistic architectural differences from bacterial IVa IVb pili. Most Tad biosynthetic genes encoded in a single locus that is widespread bacteria due facile acquisition via horizontal gene transfer. These loci experience extensive structural rearrangements, the of novel regulatory or genes, which fine-tune function. This has permitted integration into many different lifestyles, Caulobacter crescentus cell cycle, Myxococcus xanthus predation, numerous plant mammalian pathogens symbionts.

Language: Английский

Citations

10
Microbial population properties in the hierarchically structured aerobic granular sludge: Phenotype and genotype DOI
Chunli Wan, Zhengwen Li,

Liyan Deng

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 867, P. 161164 - 161164

Published: Jan. 9, 2023

Language: Английский

Citations

15
DNA modifications impact natural transformation of Acinetobacter baumannii DOI Creative Commons
Nina Vesel, Christian Iseli, Nicolas Guex

et al.

Nucleic Acids Research, Journal Year: 2023, Volume and Issue: 51(11), P. 5661 - 5677

Published: May 13, 2023

Abstract Acinetobacter baumannii is a dangerous nosocomial pathogen, especially due to its ability rapidly acquire new genetic traits, including antibiotic resistance genes (ARG). In A. baumannii, natural competence for transformation, one of the primary modes horizontal gene transfer (HGT), thought contribute ARG acquisition and has therefore been intensively studied. However, knowledge regarding potential role epigenetic DNA modification(s) on this process remains lacking. Here, we demonstrate that methylome pattern diverse strains differs substantially these marks influence fate transforming DNA. Specifically, describe methylome-dependent phenomenon impacts intra- inter-species exchange by competent strain A118. We go identify characterize an A118-specific restriction-modification (RM) system impairs transformation when incoming lacks specific methylation signature. Collectively, our work contributes towards more holistic understanding HGT in organism may also aid future endeavors tackling spread novel ARGs. particular, results suggest exchanges between bacteria share similar epigenomes are favored could guide research into identifying reservoir(s) traits multi-drug resistant pathogen.

Language: Английский

Citations

13
Assembly mechanism of a Tad secretion system secretin-pilotin complex DOI Creative Commons
Matteo Tassinari, Marta Rudzite, Alain Filloux

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 13, 2023

Abstract The bacterial T ight ad herence S ecretion ystem (TadSS) assembles surface pili that drive cell adherence, biofilm formation and predation. structure mechanism of the TadSS is mostly unknown. This includes characterisation outer membrane secretin through which pilus channelled recruitment its pilotin. Here we investigate RcpA TadD lipoprotein from Pseudomonas aeruginosa . Light microscopy reveals colocalising with in P. when heterologously expressed Escherichia coli We use cryogenic electron to determine how assemble a channel C13 C14 symmetries. Despite low sequence homology, show shares similar fold type 4 system pilotin PilF. establish C-terminal four residues bind - an interaction essential for formation. binding between appears distinct known secretin-pilotin pairings other secretion systems.

Language: Английский

Citations

13
Cryo-EM structures of type IV pili complexed with nanobodies reveal immune escape mechanisms DOI Creative Commons

David Fernandez-Martinez,

Youxin Kong,

Sylvie Goussard

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 18, 2024

Abstract Type IV pili (T4P) are prevalent, polymeric surface structures in pathogenic bacteria, making them ideal targets for effective vaccines. However, bacteria have evolved efficient strategies to evade type pili-directed antibody responses. Neisseria meningitidis prototypical pili-expressing Gram-negative responsible life threatening sepsis and meningitis. This species has several genetic modify the of its pili, changing pilin subunit amino acid sequence, nature glycosylation phosphoforms, but how these modifications affect binding at structural level is still unknown. Here, explore this question, we determine cryo-electron microscopy (cryo-EM) different sequence types with sufficiently high resolution visualize posttranslational modifications. We then generate nanobodies directed against which alter pilus function vitro vivo. Cryo-EM combination molecular dynamics simulation nanobody-pilus complexes reveals nanobody binding. Our findings shed light on impressive complementarity between used by avoid Importantly, also show that information can be make informed as countermeasures immune evasion mechanisms.

Language: Английский

Citations

5
Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system DOI Creative Commons
Simon B. Otto, Richard Servajean,

Alexandre Lemopoulos

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(11), P. 2403 - 2417.e9

Published: May 14, 2024

The bacterial type VI secretion system (T6SS) is a widespread, kin-discriminatory weapon capable of shaping microbial communities. Due to the system's dependency on contact, cellular interactions can lead either competition or kin protection. Cell-to-cell contact often accomplished via surface-exposed IV pili (T4Ps). In Vibrio cholerae, these T4Ps facilitate specific when bacteria colonize natural chitinous surfaces. However, it has remained unclear whether and, if so, how affect bacterium's T6SS-mediated killing. this study, we demonstrate that pilus-mediated be harnessed by T6SS-equipped V. cholerae kill non-kin cells under liquid growth conditions. We also show naturally occurring diversity determines likelihood cell-to-cell consequently, extent competition. To determine factors enable hinder T6SS's targeted reduction competitors carrying pili, developed physics-grounded computational model for autoaggregation. Collectively, our research demonstrates involved in impose selective burden encounters possess an active T6SS. Additionally, study underscores significance T4P protecting closely related individuals from T6SS attacks through autoaggregation and spatial segregation.

Language: Английский

Citations

5
PilY1 regulates the dynamic architecture of the type IV pilus machine in Pseudomonas aeruginosa DOI Creative Commons
Shuaiqi Guo, Yunjie Chang, Yves V. Brun

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 30, 2024

Language: Английский

Citations

5