Population genomics of Klebsiella pneumoniae

Nature Reviews Microbiology, Год журнала: 2020, Номер 18(6), С. 344 - 359

Опубликована: Фев. 13, 2020

Язык: Английский

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SARS ‐CoV‐2, bacterial co‐infections, and AMR : the deadly trio in COVID ‐19?

EMBO Molecular Medicine, Год журнала: 2020, Номер 12(7)

Опубликована: Май 26, 2020

Respiratory viral infections are well known to predispose patients bacterial co-infections and superinfections. Still, there is limited reference these in COVID-19. Do play a significant role during COVID-19? What the impact of antimicrobial resistance?

Язык: Английский

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Biofilm formation and antibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia

BMC Proceedings, Год журнала: 2019, Номер 13(S11)

Опубликована: Дек. 1, 2019

Abstract Background Klebsiella pneumoniae (K. pneumoniae) is a common cause of health-care associated infections (HAIs) and has high levels antibiotic resistance. These bacteria are well-known for their ability to produce biofilm. The purpose this study was identify the resistance pattern biofilm-producing capacity K. isolated from clinical samples in tertiary care hospital Klaten, Indonesia. Methods inpatients Soeradji Tirtonegoro Hospital Klaten June 2017 May 2018. Identification isolate done by analyzing colony morphology, microscopic examination, performing biochemical testing. Testing antibiotics susceptibility used Kirby-Bauer disk diffusion method adherence quantitative assays, respectively. Results A total 167 (17.36%) isolates were 962 bacterial during study. Most them collected patients aged more than 60 years old mainly obtained respiratory specimens (51.50%). extensively resistant antibiotics. favorable profile found only towards meropenem, amikacin, piperacillin-tazobactam, showing 1.20%; 4.79% 10.53% resistance, overall proportion multidrug-resistant 54.49%. In addition, 148 (85.63%) biofilm producers, with 45 (26.95%) as strong, 48 (28.74%) moderate, 50 (29.94%) weak producers. Conclusion demonstrated wide range

Язык: Английский

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Klebsiella pneumoniae Biofilms and Their Role in Disease Pathogenesis

Frontiers in Cellular and Infection Microbiology, Год журнала: 2022, Номер 12

Опубликована: Май 11, 2022

The ability to form biofilms is a crucial virulence trait for several microorganisms, including Klebsiella pneumoniae – Gram-negative encapsulated bacterium often associated with nosocomial infections. It estimated that 65-80% of bacterial infections are biofilm related. Biofilms complex communities composed one or more species encased in an extracellular matrix made proteins, carbohydrates and genetic material derived from the bacteria themselves as well host. Bacteria shielded immune responses antibiotics. present review discusses characteristics K. biofilms, factors affecting development, their contribution We also explore different model systems designed study formation this species. A great number contribute establishment maintenance , which highlights importance mechanism fitness. Some these molecules could be used future vaccines against bacterium. However, there still lack vivo models evaluate development disease pathogenesis. With mind, combination methodologies has potential provide detailed scenario accurately reflects steps progression natural infection.

Язык: Английский

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Klebsiella oxytoca Complex: Update on Taxonomy, Antimicrobial Resistance, and Virulence

Clinical Microbiology Reviews, Год журнала: 2021, Номер 35(1)

Опубликована: Дек. 1, 2021

Klebsiella oxytoca is actually a complex of nine species-Klebsiella grimontii, huaxiensis, michiganensis, K. oxytoca, pasteurii, spallanzanii, and three unnamed novel species. Phenotypic tests can assign isolates to the complex, but precise species identification requires genome-based analysis. The human commensal also an opportunistic pathogen causing various infections, such as antibiotic-associated hemorrhagic colitis (AAHC), urinary tract infection, bacteremia, has caused outbreaks. Production cytotoxins tilivalline tilimycin lead AAHC, while many virulence factors seen in pneumoniae, capsular polysaccharides fimbriae, have been found complex; however, their association with pathogenicity remains unclear. Among 5,724 clinical SENTRY surveillance system, rates nonsusceptibility carbapenems, ceftriaxone, ciprofloxacin, colistin, tigecycline were 1.8%, 12.5%, 7.1%, 0.8%, 0.1%, respectively. Resistance carbapenems increasing alarmingly. In addition intrinsic blaOXY, genes encoding β-lactamases varying spectra hydrolysis, including extended-spectrum β-lactamases, few CTX-M variants several TEM SHV variants, found. blaKPC-2 most common carbapenemase gene mainly on IncN or IncF plasmids. Due ability acquire antimicrobial resistance carriage multiple genes, potential become major threat health.

Язык: Английский

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Bacterial Infections and Cancer: Exploring This Association And Its Implications for Cancer Patients

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 3110 - 3110

Опубликована: Фев. 4, 2023

Bacterial infections are common in the etiology of human diseases owing to ubiquity bacteria. Such promote development periodontal disease, bacterial pneumonia, typhoid, acute gastroenteritis, and diarrhea susceptible hosts. These may be resolved using antibiotics/antimicrobial therapy some However, other hosts unable eliminate bacteria, allowing them persist for long durations significantly increasing carrier's risk developing cancer over time. Indeed, infectious pathogens modifiable factors, through this comprehensive review, we highlight complex relationship between several types. For searches were performed on PubMed, Embase, Web Science databases encompassing entirety 2022. Based our investigation, found critical associations, which causative: Porphyromonas gingivalis Fusobacterium nucleatum associated with Salmonella spp., Clostridium perfringens, Escherichia coli, Campylobacter Shigella gastroenteritis. Helicobacter pylori infection is implicated gastric cancer, persistent Chlamydia present a factor cervical carcinoma, especially patients papillomavirus (HPV) coinfection. typhi linked gallbladder pneumoniae lung etc. This knowledge helps identify adaptation strategies used by bacteria evade antibiotic/antimicrobial therapy. The article also sheds light role antibiotics treatment, consequences their use, limiting antibiotic resistance. Finally, dual as well briefly discussed, an area that help facilitate novel microbe-based therapeutics means securing improved outcomes.

Язык: Английский

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The burden of hospital acquired infections and antimicrobial resistance

Heliyon, Год журнала: 2023, Номер 9(10), С. e20561 - e20561

Опубликована: Окт. 1, 2023

The burden of Hospital care-associated infections (HCAIs) is becoming a global concern. This compounded by the emergence virulent and high-risk bacterial strains such as "ESKAPE" pathogens – (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa Enterobacter species), especially within Intensive care units (ICUs) that house immunocompromised patients. In this review, we discuss contributions AMR to increasing HCAIs provide insights into mechanisms, with particular focus on last-resort antibiotics like polymyxins. We extensively how structural modifications surface-membrane lipopolysaccharides cationic interactions influence inform AMR, subsequent severity HCAIs. highlight some phenotypic survival mechanisms against Lastly, plasmid-mediated resistance phenomenon making mitigation difficult, ICUs. review provides balanced perspective HCAIs, associated pathogens, implication factors influencing emerging mechanisms.

Язык: Английский

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Gut microbiota facilitate chronic spontaneous urticaria

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 2, 2024

Abstract Chronic spontaneous urticaria (CSU) comes with gut dysbiosis, but its relevance remains elusive. Here we use metagenomics sequencing and short-chain fatty acids metabolomics assess the effects of human CSU fecal microbial transplantation, Klebsiella pneumoniae , Roseburia hominis metabolites in vivo. microbiota displays low diversity production, high levels, negatively correlates blood levels links to disease activity. Blood lipopolysaccharide are elevated, link rapid relapse, conditional pathogenic bacteria. microbiome transfer transplantation facilitate IgE-mediated mast cell(MC)-driven skin inflammatory responses increase intestinal permeability accumulation recipient mice. Transplantation caproate administration protect mice from MC-driven inflammation. Here, show alterations, CSU, may reduce respectively,

Язык: Английский

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OmpK36-mediated Carbapenem resistance attenuates ST258 Klebsiella pneumoniae in vivo

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Сен. 2, 2019

Abstract Carbapenem-resistance in Klebsiella pneumoniae (KP) sequence type ST258 is mediated by carbapenemases (e.g. KPC-2) and loss or modification of the major non-selective porins OmpK35 OmpK36. However, mechanism underpinning OmpK36-mediated resistance consequences these changes on pathogenicity remain unknown. By solving crystal structure a clinical OmpK36 variant we provide direct structural evidence pore constriction, di-amino acid (Gly115-Asp116) insertion into loop 3, restricting diffusion both nutrients lactose) Carbapenems. In presence KPC-2 this results 16-fold increase MIC to Meropenem. Additionally, Gly-Asp impairs bacterial growth lactose-containing medium confers significant vivo fitness cost murine model ventilator-associated pneumonia. Our data suggests that continuous selective pressure imposed widespread Carbapenem utilisation hospital settings drives expansion KP expressing mutants, despite an associated cost.

Язык: Английский

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Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent

PLoS Pathogens, Год журнала: 2020, Номер 16(3), С. e1007969 - e1007969

Опубликована: Март 19, 2020

Klebsiella pneumoniae is recognized as an urgent threat to human health due the increasing isolation of multidrug resistant strains. Hypervirulent strains are a major concern their ability cause life-threating infections in healthy hosts. The type VI secretion system (T6SS) widely implicated microbial antagonism, and it mediates interactions with host eukaryotic cells some cases. In silico search for genes orthologous T6SS component effector across 700 K. genomes shows extensive diversity species. Temperature, oxygen tension, pH, osmolarity, iron levels, NaCl regulate expression encoded by hypervirulent strain. Polymyxins defensin 3 also increase activity T6SS. A screen regulators governing uncover correlation between transcription kill E. coli prey. Whereas H-NS represses T6SS, PhoPQ, PmrAB, Hfq, Fur, RpoS RpoN positively intra inter species bacterial competition. This antagonism only evident when prey possesses active PhoPQ two governs activation competitions. Mechanistically, PhoQ periplasmic domain, acid patch within, essential activate anti-fungal We have delineated contribution each individual VgrGs competition identified VgrG4 effector. DUF2345 domain sufficient intoxicate bacteria yeast. ROS generation antibacterial effects VgrG4, antitoxin Sel1E protects against toxic VgrG4. Our findings provide better understanding regulation competitions, place early event

Язык: Английский

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