The Role of Bacterial Biofilm in Antibiotic Resistance and Food Contamination

International Journal of Microbiology, Год журнала: 2020, Номер 2020, С. 1 - 10

Опубликована: Авг. 25, 2020

Biofilm is a microbial association or community attached to different biotic abiotic surfaces environments. These surface-attached communities can be found in food, medical, industrial, and natural critical problem the medical sector since it formed on implants within human tissue involved multitude of serious chronic infections. Food food processing surface become an ideal environment for biofilm formation where there are sufficient nutrients growth attachment. Therefore, these surfaces, especially becomes challenge safety health. Microorganisms encased matrix extracellular polymeric substances that act as barrier recalcitrant hostile conditions such sanitizers, antibiotics, other hygienic conditions. Generally, they persist exist environments source cross-contamination foodborne diseases. The issue with their antibiotic resistance which makes medication difficult, use physical, physiological, gene-related factors develop mechanisms. In order mitigate production controlling methods, better understand requirements aim this review article provide overview role bacterial biofilms contamination emphasizes ways its production.

Язык: Английский

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Biofilms by bacterial human pathogens: Clinical relevance - development, composition and regulation - therapeutical strategies

Microbial Cell, Год журнала: 2021, Номер 8(2), С. 28 - 56

Опубликована: Янв. 27, 2021

Notably, bacterial biofilm formation is increasingly recognized as a passive virulence factor facilitating many infectious disease processes. In this review we will focus on biofilms formed by human pathogens and highlight their relevance for diverse diseases. Along composition regulation emphasis laid the intensively studied of

Язык: Английский

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Biofilms: Formation, drug resistance and alternatives to conventional approaches

AIMS Microbiology, Год журнала: 2022, Номер 8(3), С. 239 - 277

Опубликована: Янв. 1, 2022

<abstract> <p>Biofilms are aggregates of bacteria, in most cases, which resistant usually to broad-spectrum antibiotics their typical concentrations or even higher doses. A trend increasing multi-drug resistance biofilms, responsible for emerging life-threatening nosocomial infections, is becoming a serious problem. Biofilms, however, at various sensitivity levels environmental factors and versatile infectivity depending on virulence factors. This review presents the fundamental information about biofilms: formation, antibiotic resistance, impacts public health alternatives conventional approaches. Novel developments micro-biosystems that help reveal new treatment tools by sensing characterization biofilms will also be discussed. Understanding structure, physiology properties better helps eliminate them usage appropriate control novel therapy approaches, such as anti-biofilm molecules, effective gene editing, drug-delivery systems probiotics.</p> </abstract>

Язык: Английский

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Klebsiella pneumoniae Biofilms and Their Role in Disease Pathogenesis

Frontiers in Cellular and Infection Microbiology, Год журнала: 2022, Номер 12

Опубликована: Май 11, 2022

The ability to form biofilms is a crucial virulence trait for several microorganisms, including Klebsiella pneumoniae – Gram-negative encapsulated bacterium often associated with nosocomial infections. It estimated that 65-80% of bacterial infections are biofilm related. Biofilms complex communities composed one or more species encased in an extracellular matrix made proteins, carbohydrates and genetic material derived from the bacteria themselves as well host. Bacteria shielded immune responses antibiotics. present review discusses characteristics K. biofilms, factors affecting development, their contribution We also explore different model systems designed study formation this species. A great number contribute establishment maintenance , which highlights importance mechanism fitness. Some these molecules could be used future vaccines against bacterium. However, there still lack vivo models evaluate development disease pathogenesis. With mind, combination methodologies has potential provide detailed scenario accurately reflects steps progression natural infection.

Язык: Английский

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Biofilm-Associated Multi-Drug Resistance in Hospital-Acquired Infections: A Review

Infection and Drug Resistance, Год журнала: 2022, Номер Volume 15, С. 5061 - 5068

Опубликована: Авг. 1, 2022

Biofilm-related multi-drug resistance (MDR) is a major problem in hospital-acquired infections (HAIs) that increase patient morbidity and mortality rates economic burdens such as high healthcare costs prolonged hospital stay. This review focuses on the burden of bacterial biofilm settings, their impact emergence MDR HAIs, detection methods, recent approaches against biofilms, future perspectives. The prevalence biofilm-associated among HAIs ranges from 17.9% to 100.0% worldwide. predominant isolates causing recently published studies were S. aureus, A. baumannii, K. pneumoniae, P. aeruginosa. In addition use qualitative quantitative methods detect formation, advanced PCR-based techniques have been performed for detecting genes. Although there are suggested therapeutic strategies further confirmation efficacy vivo application antibiotics targeting genes/proteins minimize treatment failure required future.

Язык: Английский

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Facile Green Synthesis of Silver Nanoparticles Using Aqueous Leaf Extract of Origanum majorana with Potential Bioactivity against Multidrug Resistant Bacterial Strains

Crystals, Год журнала: 2022, Номер 12(5), С. 603 - 603

Опубликована: Апрель 25, 2022

The high prevalence of nosocomial bacterial resistance contributes to significant mortality and morbidity around the world; thus, finding novel antibacterial agents is vital concern. Accordingly, present study attempted synthesize silver nanoparticles (AgNPs) using a green approach. Aqueous leaf extract Origanum majorana was used AgNPs efficiency against multidrug resistant strains detected. Characterization biogenic performed ultraviolet-visible spectrophotometry (UV-Vis), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared (FT-IR) analysis, diffraction analysis (XRD). disc diffusion method detect activity three multidrug-resistant strains. Preliminary UV-Vis revealed biosynthesis due peak formation at 374 nm, corresponding surface plasmon resonance (SPR) AgNPs. TEM micrographs detected synthesis small with an average particle size 26.63 nm. EDX presence following elements: oxygen (3.69%), carbon (2.93%), aluminum (1.29), silicon (2.83%), chloride (17.89%), (71.37%). Furthermore, XRD peaks 2 theta (θ) degrees 38.18°, 44.36°, 64.35°, 77.54°, assigned planes crystals (111), (200), (220), (311), respectively. Collectively, these findings affirm potential physicochemical characteristics. antimicrobial indicated that Klebsiella pneumoniae strain most susceptible concentrations 50 100 µg/disk, inhibitory zones 21.57 24.56 mm, minimum concentration (MIC) found be 10 µg/mL, while bactericidal (MBC) 20 µg/mL. In conclusion, aqueous O. mediated sized AgNPs, effectiveness pathogens.

Язык: Английский

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Development of Antibiofilm Therapeutics Strategies to Overcome Antimicrobial Drug Resistance

Microorganisms, Год журнала: 2022, Номер 10(2), С. 303 - 303

Опубликована: Янв. 27, 2022

A biofilm is a community of stable microorganisms encapsulated in an extracellular matrix produced by themselves. Many types that are found on living hosts or the environment can form biofilms. These include pathogenic bacteria serve as reservoir for persistent infections, and culpable leading to broad spectrum chronic illnesses emergence antibiotic resistance making them difficult be treated. The absence biofilm-targeting antibiotics drug discovery pipeline indicates unmet opportunity designing new inhibitors antimicrobial agents using various strategies targeting distinct stages formation. available control formation enzymes proteins specific microorganism those involved adhesion pathways resistant This review primarily focuses recent advances responsible identifying myriad antibiofilm their mechanism inhibition, including polymeric substance synthesis inhibitors, quorum sensing efflux pump cyclic diguanylate inhibitors. Furthermore, we present structure-activity relationships (SAR) these agents, recently discovered nature-derived bioactive scaffolds, synthetic small molecules, peptides, compounds isolated from fungi, non-proteinogenic amino acids antibiotics. We hope fuel interest focus research efforts development uniquely complex, physical chemical heterogeneous biofilms through multipronged approach combinatorial therapeutics more effective management across diseases.

Язык: Английский

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Medical Device-Associated Biofilm Infections and Multidrug-Resistant Pathogens

Pathogens, Год журнала: 2024, Номер 13(5), С. 393 - 393

Опубликована: Май 8, 2024

Medical devices such as venous catheters (VCs) and urinary (UCs) are widely used in the hospital setting. However, implantation of these is often accompanied by complications. About 60 to 70% nosocomial infections (NIs) linked biofilms. The main complication ability microorganisms adhere surfaces form biofilms which protect them help persist host. Indeed, crossing skin barrier, insertion VC inevitably allows flora or accidental environmental contaminants access underlying tissues cause fatal complications like bloodstream (BSIs). In fact, 80,000 central catheters-BSIs (CVC-BSIs)-mainly occur intensive care units (ICUs) with a death rate 12 25%. Similarly, catheter-associated tract (CA-UTIs) most commonlyhospital-acquired (HAIs) worldwide.These represent up 40% NIs.In this review, we present summary biofilm formation steps. We provide an overview two important clinical settings medical devices, namely catheter-asociated (CA-BSIs) (CA-UTIs), highlight also multidrug resistant bacteria implicated infections. Furthermore, draw attention toseveral useful prevention strategies, advanced antimicrobial antifouling approaches developed reduce bacterial colonization on catheter incidence catheter-related

Язык: Английский

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Global Prevalence of Nosocomial Multidrug-Resistant Klebsiella pneumoniae: A Systematic Review and Meta-Analysis

Antibiotics, Год журнала: 2021, Номер 10(12), С. 1508 - 1508

Опубликована: Дек. 8, 2021

The emergence of nosocomial multidrug-resistant Klebsiella pneumoniae is an escalating public health threat worldwide. prevalence infections due to K. was recorded up 10%. In this systematic review and meta-analysis, which were conducted according the guidelines Preferred Reporting Items for Systematic Review Meta-Analysis, 1092 articles screened from four databases 47 studies fulfilled selected criteria. By performing a random-effect model, pooled estimated at 32.8% (95% CI, 23.6–43.6), with high heterogeneity (I2 98.29%, p-value < 0.001). pathogen few related discussed, raising awareness spread in healthcare setting. expected increase globally future, best treatments treating preventing should be acknowledged by staff.

Язык: Английский

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Association between Biofilm-Production and Antibiotic Resistance in Uropathogenic Escherichia coli (UPEC): An In Vitro Study

Diseases, Год журнала: 2020, Номер 8(2), С. 17 - 17

Опубликована: Июнь 7, 2020

Urinary tract infections (UTIs) are among the most common requiring medical attention worldwide. The production of biofilms is an important step in UTIs, not only from a mechanistic point view, but this may also confer additional resistance, distinct other aspects multidrug resistance (MDR). A total two hundred and fifty (

Язык: Английский

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