Endometriosis and the Role of Pro-Inflammatory and Anti-Inflammatory Cytokines in Pathophysiology: A Narrative Review of the Literature

Diagnostics, Год журнала: 2024, Номер 14(3), С. 312 - 312

Опубликована: Янв. 31, 2024

Endometriosis is a chronic inflammatory disease, which explains the pain that such patients report. Currently, we are faced with ineffective, non-invasive diagnostic methods and treatments come multiple side effects high recurrence rates for both disease pain. These reasons why exploring possibility of involvement pro-inflammatory anti-inflammatory molecules in process appearance endometriosis. Cytokines play an important role progression endometriosis, influencing cell proliferation differentiation. Pro-inflammatory found intrafollicular fluid. They have impact on number mature optimal-quality oocytes. affects fertility, endometriosis embryo transfer during vitro fertilization (IVF) being investigated several studies. Furthermore, reciprocal influence between cytokines their pathogenesis has been assessed. Today, can affirm roles survival, growth, differentiation, invasion, angiogenesis, immune escape, provides perspective approaching future clinical implications be used as biomarkers or therapy.

Язык: Английский

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The role of regulatory T-cells in the development of endometriosis

Human Reproduction, Год журнала: 2024, Номер 39(7), С. 1367 - 1380

Опубликована: Май 16, 2024

Abstract Endometriosis is a benign disease of the female reproductive tract, characterized by process chronic inflammation and alterations in immune response. It estimated to affect 2–19% women general population commonly associated with symptoms pelvic pain infertility. Regulatory T cells (Treg) are subpopulation lymphocytes that potent suppressors inflammatory response, essential preventing destructive immunity all tissues. In endometriosis, several studies have investigated possible role Treg development disease. Most date heterogeneous methodology based on small number cases, which means it impossible define their exact at present. Based current knowledge, seems disturbed homeostasis, leading increased systemic local within ectopic eutopic endometrium, present who eventually develop endometriosis. also evident different subsets human roles suppressing Recent patients endometriosis naive/resting FOXP3lowCD45RA+ cells, upon cell receptor stimulation, differentiate into activated/effector FOXP3highCD45RA− strong immunosuppressive activity. addition, critical factors controlling expression Treg/effector genes, including reactive oxygen species heme-responsive master transcription factor BACH2, were found be upregulated endometriotic lesions. As shown recently for cancer microenvironments, microbial may contribute composition FOXP3+ subpopulations Furthermore, cytokines, such as IL-7, control homeostasis through tyrosine phosphorylation STAT5 signalling pathway, been dysregulated. To better understand future should use clear definitions Tregs along specific characterization non-Treg (FOXP3lowCD45RA−) fraction, itself mixture follicular producing cytokines.

Язык: Английский

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Involvement of RBP-J interacting and tubulin-associated protein in the distribution of protein regulator of cytokinesis 1 in mitotic spindles

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 12

Опубликована: Янв. 7, 2025

The protein regulator of cytokinesis 1 (PRC1) is a key microtubule crosslinking and bundling, which crucial for spindle formation cytokinesis. RITA, the RBP-J interacting tubulin-associated protein, associated protein. We have reported that RITA localizes to mitotic spindles modulating dynamics stability as well poles affecting activity Aurora A. As defective chromosome congression segregation are most remarkable features cells depleted we aimed explore further potential related mechanisms, using various cellular molecular techniques, including clustered regularly interspaced short palindromic repeats technique/deactivated CRISPR-associated 9 (CRISPR/dCas9), mass spectrometry, confocal microscopy, immunofluorescence, immunoprecipitation Western blot analysis. Here, show FLAG-RITA precipitates PRC1 tubulin, these two proteins co-localize in central region spindle. Reduction enlarges staining area Its suppression reduces inter-centromere distance metaphase cells. Interestingly, bundles often less organized non-parallel pattern, evidenced by increased angles, relative corresponding separating chromosomes. These data suggest novel role distribution its deregulation may contribute movement during mitosis. both closely with malignant progression, work required elucidate detailed mechanisms acts modulator

Язык: Английский

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Raman spectroscopy and bioinformatics-based identification of key genes and pathways capable of distinguishing between diffuse large B cell lymphoma and chronic lymphocytic leukemia

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 25, 2025

Diffuse large B-cell lymphoma (DLBCL) and chronic lymphocytic leukemia (CLL) are subtypes of non-Hogkin (NHL) that generally distinct form one cases, but the transformation these diseases into other is possible. Some patients with CLL, for instance, have potential to develop Richter such they diagnosed a rare, invasive DLBCL subtype. In this study, bioinformatics analyses two NHL were conducted, identifying key patterns gene expression then experimentally validating results. Disease-related datasets from GEO database used identify differentially expressed genes (DEGs) DEG functions examined using GO analysis protein-protein interaction network construction. This strategy revealed many up- down-regulated DEGs, functional enrichment as being closely associated inflammatory immune response activity. PPI evaluation clustered modules indicated top 10 involved in disease onset development. Serological significantly higher ALB, TT, WBC levels CLL relative patients, whereas opposite was true respect TG, HDL, GGT, ALP, ALT, NEUT% levels. comparison groups, healthy control samples demonstrated signals protein peak positions (621, 643, 848, 853, 869, 935, 1003, 1031, 1221, 1230, 1260, 1344, 1443, 1446, 1548, 1579, 1603, 1647 cm-1), nucleic acid (726, 781, 786, 1078, 1190, 1415, 1573, 1579 beta carotene (957, 1155, 1162 carbohydrate (842 collagen (1345 lipid 1119, 1285, 1299, 1437, 1446 cm-1) compared groups. Verification patient yielded results consistent findings derived analyses, highlighting their relevance diagnosing treating forms NHL. Together, identified pathways both CLL. The set molecular markers established herein can aid diagnosis prognostic evaluation, providing valuable foundation therapeutic application.

Язык: Английский

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Designed peptide binders and nanobodies as PROTAC starting points for targeted degradation of PCNA and BCL6

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142667 - 142667

Опубликована: Март 1, 2025

Язык: Английский

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Understanding endometriosis from an immunomicroenvironmental perspective

Chinese Medical Journal, Год журнала: 2023, Номер unknown

Опубликована: Июль 13, 2023

Endometriosis, a heterogeneous, inflammatory, and estrogen-dependent gynecological disease defined by the presence growth of endometrial tissues outside lining uterus, affects approximately 5-10% reproductive-age women, causing chronic pelvic pain reduced fertility. Although etiology endometriosis is still elusive, emerging evidence supports idea that immune dysregulation can promote survival retrograde debris. Peritoneal macrophages natural killer (NK) cells exhibit deficient cytotoxicity in endometriotic microenvironment, leading to inefficient eradication refluxed fragments. In addition, imbalance T-cell subtypes results aberrant cytokine production inflammation, which contribute development. it remains uncertain whether represents an initial cause or merely secondary enhancer endometriosis, therapies targeting altered pathways satisfactory effects preventing onset progression. Here, we summarize phenotypic functional alterations focusing on their interactions with microbiota endocrine nervous systems, how these symptomology endometriosis.

Язык: Английский

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Evaluating the role of DNA methyltransferases in trophoblast fusion and preeclampsia development: Insights from methylation‐regulated genes

The FASEB Journal, Год журнала: 2024, Номер 38(13)

Опубликована: Июнь 15, 2024

Abstract The etiology of preeclampsia (PE), a complex and multifactorial condition, remains incompletely understood. DNA methylation, which is primarily regulated by three methyltransferases (DNMTs), DNMT1, DNMT3A, DNMT3B, plays vital role in early embryonic development trophectoderm differentiation. Yet, how DNMTs modulate trophoblast fusion PE unclear. In this study, we found that the expression was downregulated during cells fusion. Downregulation observed reconstruction denuded syncytiotrophoblast (STB) layer placental explants. Additionally, overexpression inhibited Conversely, treatment with methylation inhibitor 5‐aza‐CdR decreased promoted A combined analysis data gene transcriptome obtained from primary cytotrophoblasts (CTBs) process identified 104 potential methylation‐regulated differentially expressed genes (MeDEGs) upregulated due to demethylation, including CD59 , TNFAIP3 SDC1 CDK6 . transcription regulation region (TRR) showed hypomethylation induction 5‐aza‐CdR, facilitated CREB recruitment thereby participated regulating More importantly, clinical correlation abnormal increase may be involved PE. This study contribute PE, offering novel perspective on epigenetics its implication development.

Язык: Английский

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The Role of MFN2 in Regulating Endothelial Cell Senescence: Implications for Vascular Aging

iScience, Год журнала: 2024, Номер 27(9), С. 110809 - 110809

Опубликована: Авг. 24, 2024

Endothelial cell dysfunction contributes to age-related vascular diseases. Analyzing public databases and mouse tissues, we found decreased MFN2 expression in senescent endothelial cells angiotensin II-treated aortas. In human cells, Ang II reduced while increasing senescence markers P21 P53.

Язык: Английский

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The N-Terminal Part of Drosophila CP190 Is a Platform for Interaction with Multiple Architectural Proteins

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15917 - 15917

Опубликована: Ноя. 2, 2023

CP190 is a co-factor in many Drosophila architectural proteins, being involved the formation of active promoters and insulators. contains N-terminal BTB/POZ (Broad-Complex, Tramtrack Bric brac/POxvirus Zinc finger) domain adjacent conserved regions protein interactions. Here, we examined functional roles these domains vivo. The best-characterized proteins with insulator functions, Pita, Su(Hw), dCTCF, interacted predominantly BTB CP190. Due to difficulty mutating domain, obtained transgenic line expressing chimeric human Kaiso. Another group M1BP, Opbp, ZIPIC, one or both highly part Transgenic lines D. melanogaster mutants deletion each were obtained. results showed that mutant only partially compensated for functions CP190, weakly binding selective chromatin sites. Further analysis confirmed essential role recruitment regulatory associated proteins. We also found was sufficient recruiting Z4 Chromator successfully achieving opening. Taken together, our previous studies region platform simultaneous interaction various DNA-binding transcription complexes.

Язык: Английский

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Potential Significance of Serum Autoantibodies to Endometrial Antigens, α-Enolase and Hormones in Non-Invasive Diagnosis and Pathogenesis of Endometriosis

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15578 - 15578

Опубликована: Окт. 25, 2023

The objective of the study was to evaluate profile serum autoantibodies and their diagnostic pathogenetic significance in ovarian endometrioma (OEM) deep infiltrative endometriosis (DIE). enrolled 74 patients with (Group 1), including 53 OEM (Subgroup 1a); 21 DIE without lesions 1b); 27 2). diagnosis confirmed by laparoscopic surgery histologic examination resected tissues. Antibodies (M, G) tropomyosin 3 (TPM), tropomodulin (TMOD), α-enolase (ENO), estradiol (E2), progesterone (PG), human chorionic gonadotropin (hCG) were identified blood using modified ELISA. In endometriosis, antibodies endometrial antigens, hormones, ENO detected more often than antiphospholipid antinuclear antibodies. Higher levels IgM TPM, hCG, E2, PG IgG TMOD, ENO, hCG found Subgroup 1a compared Group 2. PG, E2 had a high value for (AUC > 0.7), TPM having highest sensitivity specificity (73.6% 81.5%). 1b, only higher These DIE. Thus, is associated α-enolase, steroid, gonadotropic hormones. A wider spectrum have potential infertility.

Язык: Английский

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