Journal of Inflammation Research,
Год журнала:
2024,
Номер
Volume 17, С. 10663 - 10679
Опубликована: Дек. 1, 2024
This
study
aims
to
investigate
alterations
in
immune
cell
counts
within
preovulatory
follicles
of
patients
with
poor
ovarian
response
(POR)
during
assisted
reproductive
technology
(ART),
classified
according
the
POSEIDON
criteria.
single-centre
cross-sectional
included
543
women
undergoing
IVF/ICSI
treatment,
selected
based
on
specific
inclusion
and
exclusion
criteria:
292
normal
251
response.
Follicular
fluid
(FF)
was
collected
day
oocyte
retrieval
analysed
by
flow
cytometry
determine
proportions
macrophages
(Mφs),
M1
M2
Mφs,
T
cells
(CD4
CD8
cells),
dendritic
(DCs),
including
type
1
conventional
(cDC1)
2
(cDC2),
neutrophils.
Multivariable
logistic
regression
assessed
relationship
between
POR,
Pearson
correlation
determined
associations
number
retrieved
oocytes,
receiver
operating
characteristic
(ROC)
curves
evaluated
predictive
power
for
POR.
Immune
accounted
52.57%
(±23.90%)
total
population
follicular
microenvironment,
which
approximately
equal
that
granulosa
cells,
Mφs
being
most
abundant,
followed
sequentially
DCs,
In
overall
infiltration
microenvironment
decreased,
whereas
polarization
increased.
increased,
a
decrease
CD4/CD8
ratio.
Both
cDC1
cDC2
were
significantly
elevated.
Moreover,
multivariable
revealed
macrophage
count,
CD4
count
independent
predictors
Notably,
showed
largest
area
under
ROC
curve,
suggesting
its
strong
potential
as
biomarker
predicting
The
proportion
altered
These
findings
suggest
dynamics
may
play
crucial
role
determining
prognosis,
indicating
targeted
immunomodulatory
strategies
could
be
considered
future
therapeutic
approaches.
Reproductive Biology and Endocrinology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Окт. 16, 2024
Restoring
the
function
of
ovary
is
important
for
chemotherapy-induced
ovarian
failure
(COF)
patients.
Stem
cell
and
extracellular
vesicles
(EVs)
therapy
show
promise
but
need
further
improvement.
Frontiers in Endocrinology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 14, 2024
Introduction
The
mechanisms
leading
to
ovarian
primordial
follicle
depletion
following
gonadotoxic
chemotherapy
with
cyclophosphamide
and
other
cytotoxic
drugs
are
currently
understood
through
two
main
explanatory
theories:
apoptosis
over-activation.
Discrepancies
between
the
findings
of
different
studies
investigating
these
do
not
allow
reach
a
firm
conclusion.
heterogeneity
cell
types
in
ovaries
their
degrees
sensitivity
damage,
cell-cell
interactions,
periodical
profile
differences,
model
age-dependent
differences
exposure
durations
tested
may
partially
explain
discrepancies
among
studies.
Methods
This
study
used
intact
prepubertal
mice
culture
as
model,
which
most
follicles
follicles.
Histological
transcriptional
analyses
exposed
active
metabolite
4-hydroperoxycyclophosphamide
(4-HC)
were
carried
out
via
time-course
experiment
at
8,
24,
48,
72
h.
Results
4-HC
treated
showed
significant
decrease
density
24
h,
along
DNA
damage
(TUNEL)
overexpressed
signals
(cleaved-poly
ADP
ribose
polymerase
immunohistochemistry
western
blotting).
Meanwhile
treatment
significantly
up-regulated
H2ax,
Casp
6,
Noxa,
Bax
ovaries,
Puma
(FISH).
Discussion
Our
results
indicated
that
cyclophosphamide-induced
acute
was
mainly
related
apoptotic
pathways.
No
evidence
activation
found,
neither
changes
expression
genes
nor
growing
Further
validation
protein
level
4-HC-treated
h
confirmed
observations.
Journal of Inflammation Research,
Год журнала:
2024,
Номер
Volume 17, С. 10663 - 10679
Опубликована: Дек. 1, 2024
This
study
aims
to
investigate
alterations
in
immune
cell
counts
within
preovulatory
follicles
of
patients
with
poor
ovarian
response
(POR)
during
assisted
reproductive
technology
(ART),
classified
according
the
POSEIDON
criteria.
single-centre
cross-sectional
included
543
women
undergoing
IVF/ICSI
treatment,
selected
based
on
specific
inclusion
and
exclusion
criteria:
292
normal
251
response.
Follicular
fluid
(FF)
was
collected
day
oocyte
retrieval
analysed
by
flow
cytometry
determine
proportions
macrophages
(Mφs),
M1
M2
Mφs,
T
cells
(CD4
CD8
cells),
dendritic
(DCs),
including
type
1
conventional
(cDC1)
2
(cDC2),
neutrophils.
Multivariable
logistic
regression
assessed
relationship
between
POR,
Pearson
correlation
determined
associations
number
retrieved
oocytes,
receiver
operating
characteristic
(ROC)
curves
evaluated
predictive
power
for
POR.
Immune
accounted
52.57%
(±23.90%)
total
population
follicular
microenvironment,
which
approximately
equal
that
granulosa
cells,
Mφs
being
most
abundant,
followed
sequentially
DCs,
In
overall
infiltration
microenvironment
decreased,
whereas
polarization
increased.
increased,
a
decrease
CD4/CD8
ratio.
Both
cDC1
cDC2
were
significantly
elevated.
Moreover,
multivariable
revealed
macrophage
count,
CD4
count
independent
predictors
Notably,
showed
largest
area
under
ROC
curve,
suggesting
its
strong
potential
as
biomarker
predicting
The
proportion
altered
These
findings
suggest
dynamics
may
play
crucial
role
determining
prognosis,
indicating
targeted
immunomodulatory
strategies
could
be
considered
future
therapeutic
approaches.
