Snorkel‐tag based affinity chromatography for recombinant extracellular vesicle purification

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(10)

Опубликована: Окт. 1, 2024

Abstract Extracellular vesicles (EVs) are lipid nanoparticles and play an important role in cell‐cell communications, making them potential therapeutic agents allowing to engineer for targeted drug delivery. The expanding applications of EVs next generation medicine is still limited by existing tools scaling standardized EV production, single tracing analytics, thus provide only a snapshot tissue‐specific cargo information. Here, we present the Snorkel‐tag, which have genetically fused surface marker protein CD81, series tags with additional transmembrane domain be displayed on surface, resembling snorkel. This system enables affinity purification from complex matrices non‐destructive form while maintaining characteristics terms profiles, associated miRNA patterns uptake into model cell line. Therefore, consider Snorkel‐tag widely applicable tool research, efficient preparation standards reference materials, or dissecting different markers when fusing other tetraspanins vitro vivo.

Язык: Английский

Isolation, identification, and challenges of extracellular vesicles: emerging players in clinical applications

APOPTOSIS, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 10, 2024

Язык: Английский