Oligomeric proanthocyanidins ameliorates osteoclastogenesis through reducing OPG/RANKL ratio in chicken's embryos
Gengsheng Yu,
Xiaohui Fu,
Anqing Gong
и другие.
Poultry Science,
Год журнала:
2024,
Номер
103(6), С. 103706 - 103706
Опубликована: Март 30, 2024
Skeletal
disorders
can
seriously
threaten
the
health
and
performance
of
poultry,
such
as
tibial
dyschondroplasia
(TD)
osteoporosis
(OP).
Oligomeric
proanthocyanidins
(OPC)
are
naturally
occurring
polyphenolic
flavonoid
compounds
that
be
used
potential
substances
to
improve
bone
growth
poultry.
Eighty
7-day-old
green-eggshell
yellow
feather
layer
chickens
were
randomly
divided
into
four
groups:
basal
diet
supplementation
with
25,
50
100
mg/kg
OPC.
The
results
have
indicated
parameters
significantly
improved
OPC
in
vivo,
including
volume
(BV),
mineral
density
(BMD)
activities
antioxidative
enzymes,
but
ratio
osteoprotegerin
(OPG)/receptor
activator
NF-κB
(RANK)
ligand
(RANKL)
was
decreased.
Furthermore,
primary
marrow
mesenchymal
stem
cells
(BMSCs)
monocytes/macrophages
(BMMs)
successfully
isolated
from
femur
tibia
chickens,
co-cultured
differentiate
osteoclasts
vitro.
osteogenic
differentiation
derived
BMSCs
promoted
treatment
high
concentrations
(10,
20
40
µmol/L)
groups
vitro,
emerging
inhibition
osteoclastogenesis
by
increasing
OPG/RANKL.
In
contrary,
also
low
(2.5,
5
10
groups,
enhanced
decreasing
OPG/RANKL
addition,
activity
inhibited
OPG
autophagy
Dietary
alter
balance
osteoblast
osteoclast,
thereby
improving
chickens.
Язык: Английский
FAM98 Family Proteins Play Distinct Roles in Osteoclastogenesis and Bone Resorption
Biology,
Год журнала:
2025,
Номер
14(1), С. 45 - 45
Опубликована: Янв. 9, 2025
There
are
three
FAM98
family
proteins
(FAM98A/B/C)
in
humans
and
mice.
Their
physiological
functions
remain
largely
unknown.
We
have
previously
reported
that
Fam98a
interacts
with
Plekhm1
murine
osteoclasts
lysosome
trafficking/secretion
bone
resorption
vitro.
In
this
study,
we
found
all
Fam98
genes
were
expressed
precursor
mature
osteoclasts.
While
the
knockdown
of
Fam98c
by
a
specific
short-hairpin
RNA
(shRNA)
osteoclast
precursors
attenuated
osteoclastogenesis,
depletion
Fam98b
an
shRNA
specifically
disrupted
trafficking
phenotypes
similar
to
shRNA-knockdown
our
previous
study.
Loss
myeloid
was
dispensable
for
trabecular
cortical
mass
mice,
as
well
osteoclastogenesis/bone
vitro,
possibly
due
compensation
increased
expression
Fam98a-null
These
findings
indicate
play
distinct
roles
osteoclastogenesis
function
need
further
investigation
future
studies.
Язык: Английский
Geranylgeranyl diphosphate synthase inhibition impairs osteoclast differentiation, morphology and resorptive activity
JBMR Plus,
Год журнала:
2024,
Номер
9(1)
Опубликована: Окт. 23, 2024
Abstract
Nitrogen
bisphosphonates,
such
as
zoledronic
acid,
target
the
enzyme
farnesyl
diphosphate
synthase
(FDPS)
in
isoprenoid
biosynthetic
pathway
(IBP),
and
are
frontline
treatment
for
osteolytic
bone
diseases.
A
strong
affinity
of
these
agents
limits
their
distribution
out
skeleton.
Geranylgeranyl
(GGDPS)
is
directly
downstream
to
FDPS
IBP
novel
GGDPS
inhibitors
RAM2061
have
been
shown
key
drug-like
features
including
prolonged
half-life,
metabolic
stability,
systemic
distribution.
Furthermore,
exerts
anti-neoplastic
benefits
mouse
models
multiple
myeloma
Ewing
sarcoma.
Therefore,
we
interested
determining
potential
impact
on
osteoclast
biology
remodeling.
Studies
utilizing
undifferentiated
RAW264.7
cells
demonstrated
that
with
depletes
geranylgeranyl
diphosphate,
impairs
protein
geranylgeranylation,
induces
markers
unfolded
response
apoptosis.
Differentiation
mature
osteoclasts
disrupted
by
RAM2061,
resulting
decreased
numbers
osteoclasts,
altered
morphology,
tartrate-resistant
acid
phosphatase
activity.
Treatment
fully
differentiated
led
resorptive
Confocal
microscopy
studies
revealed
disrupts
Cdc42
localization,
inhibiting
proper
actin
ring
formation
osteoclasts.
No
significant
turnover
or
histomorphology
was
observed
following
a
3-week
CD-1
mice
although
were
observed.
Liquid
chromatography-tandem
mass
spectrometry
confirmed
accumulation
from
vivo
well
hydroxyapatite
binding
vitro.
In
conclusion,
first
demonstrate
anti-osteoclastic
activity
inhibitor
support
future
exploring
therapeutic
benefit
this
therapy
setting
pathological
Язык: Английский
The emerging role of Rab proteins in osteoclast organelle biogenesis and function
Biochemical Society Transactions,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 6, 2024
Rab
GTPase
proteins
have
been
extensively
studied
for
their
roles
in
regulating
vesicle
and
organelle
dynamics.
Among
the
∼60
subtypes
mammalian
cells,
several
Rabs
reported
to
play
crucial
osteoclast
biogenesis
function.
In
this
review,
we
aim
provide
an
update
on
recently
described
GTPases,
Rab11,
Rab32,
Rab44,
Rab38,
as
well
Rab7,
Rab3D
Rab27A
formation
Язык: Английский