Bacterial dynamics of the plastisphere microbiome exposed to sub-lethal antibiotic pollution

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Май 24, 2024

Abstract Background Antibiotics and microplastics are two major aquatic pollutants that have been associated to antibiotic resistance selection in the environment considered a risk human health. However, little is known about interaction of these at environmental concentrations response microbial communities plastisphere sub-lethal pollution. Here, we describe bacterial dynamics underlying this surface water bacteria community, resistome mobilome level using combination methods (next-generation sequencing qPCR), targets (16S rRNA gene, pre-clinical clinical class 1 integron cassettes metagenomes), technologies (short long read sequencing), assembly approaches (non-assembled reads, genome assembly, bacteriophage plasmid assembly). Results Our results show shift community antibiotics microbiome compared subpopulations respond differently microplastic The showed an increased tolerance selected different (ARB) genes (ARGs). Several metagenome assembled genomes (MAGs) derived from antibiotic-exposed contained ARGs, virulence factors, involved conjugation. These include Comamonas, Chryseobacterium, opportunistic pathogen Stenotrophomonas maltophilia , other MAGs belonging genera infections, such as Achromobacter. abundance integron-associated ciprofloxacin gene aac(6’)-Ib-cr under exposure both freshwater plastisphere. Regarding mobilome, although no significant changes ARG load integrons plasmids were observed polluted samples, identified three ARG-containing viral contigs integrated into prophages. Conclusions This study illustrates how selective nature influences pressure. here help define role its impact on maintenance with anthropogenic pollutants. research highlights need evaluate complex scenarios combined stresses.

Язык: Английский

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Exploration of the feasibility of clinical application of phage treatment for multidrug-resistant Serratia marcescens -induced pulmonary infection

Emerging Microbes & Infections, Год журнала: 2025, Номер 14(1)

Опубликована: Янв. 7, 2025

Serratia marcescens (S. marcescens) commonly induces refractory infection due to its multidrug-resistant nature. To date, there have been no reports on the application of phage treatment for S. infection. This study was conducted explore feasibility in treating by collaborating with a 59-year-old male patient pulmonary marcescens. Our experiments included three domains: i) selection appropriate phage, ii) verification efficacy and safety selected iii) confirmation phage-bacteria interactions. results showed that Spe5P4 is Treatment good efficacy, manifested as amelioration symptoms, hydrothorax examinations, chest computed tomography findings. Phage did not worsen hepatic renal function, immunity-related indices, or indices routine blood examination. It induce deteriorate drug resistance involved antibiotics. Importantly, adverse events were reported during follow-up periods. Thus, satisfactory safety. Finally, we found increase bacterial load, cytotoxicity, virulence, marcescens, indicating interactions between which are useful future against work provides evidence working basis further infections. We also provided methodological investigating clinical infections future.

Язык: Английский

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Phage‐derived proteins: Advancing food safety through biocontrol and detection of foodborne pathogens

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2025, Номер 24(2)

Опубликована: Фев. 3, 2025

Abstract The emergence of antimicrobial‐resistant foodborne pathogens poses a continuous health risk and economic burden as they can easily spread through contaminated food. Therefore, the demand for new antimicrobial agents to address this problem is steadily increasing. Similarly, development rapid, sensitive, accurate pathogen detection tools prerequisite ensuring food safety. Phage‐derived proteins have become innovative combating these because their potent activity host specificity. Phage are relatively free from regulation compared phages per se, there no concerns about transduction harmful genes. With recent progress in next‐generation sequencing technology, analysis phage genomes has more accessible, numerous with potential biocontrol been identified. This review provides comprehensive overview protein research on safety 2006 present, pivotal period marked by certification Generally Recognized As Safe (GRAS). Emphasizing advancements, we investigated diverse applications various purposes. While highlighting successful implementation proteins, also current bottlenecks propose strategies overcome challenges. By summarizing state phage‐derived contributes deeper understanding effective detecting pathogens.

Язык: Английский

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Therapeutic efficacy of a K5-specific phage and depolymerase against Klebsiella pneumoniae in a mouse model of infection

Veterinary Research, Год журнала: 2024, Номер 55(1)

Опубликована: Май 7, 2024

Klebsiella pneumoniae has become one of the most intractable gram-negative pathogens infecting humans and animals due to its severe antibiotic resistance. Bacteriophages protein products derived from them are receiving increasing amounts attention as potential alternatives antibiotics. In this study, we isolated investigated characteristics a new lytic phage, P1011, which lyses K5 K. specifically among 26 serotypes. The K5-specific capsular polysaccharide-degrading depolymerase dep1011 was identified expressed. By establishing murine infection models using bovine strain B16 (capable supporting phage proliferation) human KP181 (incapable sustaining expansion), explored safety efficacy treatments against pneumoniae. Phage P1011 resulted in 60% survival rate mice challenged with multiplication, concurrently lowering bacterial burden their blood, liver, lungs. Unexpectedly, even when confronted bacteria impervious therapy markedly decreased number viable organisms. protective significantly better than that phage. achieved 100% both treatment groups regardless propagation compatibility. These findings indicated might be used antibacterial agents control infection.

Язык: Английский

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Mucosal-adapted bacteriophages as a preventive strategy for a lethal Pseudomonas aeruginosa challenge in mice

Communications Biology, Год журнала: 2025, Номер 8(1)

Опубликована: Янв. 6, 2025

Abstract Pseudomonas aeruginosa is an emergent threat due to the antimicrobial resistance crisis. Bacteriophages (phages) are promising agents for phage therapy approaches against P. . It has been proposed that metazoans harbor phages on their mucosal surfaces, and this could be exploited rational design of prophylactic therapy. The goal study was evaluate potential phage-mucus interaction prevent infections caused by We isolated two capable infecting Both similar in morphology closely related genetically. However, VAC3 more efficient replicating mucin-exposed vitro preferentially held respiratory tract C57BL/6 mice. Pre-treatment with protects mice from a lethal dose while VAC1 does not. This shows adapted conditions have applied as measures ESKAPE pathogen.

Язык: Английский

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Prophage-DB: a comprehensive database to explore diversity, distribution, and ecology of prophages

Environmental Microbiome, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 13, 2025

Abstract Background Viruses that infect prokaryotes (phages) constitute the most abundant group of biological agents, playing pivotal roles in microbial systems. They are known to impact community dynamics, ecology, and evolution. Efforts document diversity, host range, infection effects bacteriophage on cell metabolism extremely underexplored. Phages classified as virulent or temperate based their life cycles. Temperate phages adopt lysogenic mode infection, where genome integrates into forming a prophage. Prophages enable viral replication without lysis, often contribute novel beneficial traits genome. Current phage research predominantly focuses lytic phages, leaving significant gap knowledge regarding prophages, including biology, ecological roles. Results Here we develop describe Prophage-DB, database proteins, associated metadata will serve resource for genomics ecology. To create database, identified characterized prophages from genomes three largest publicly available databases. We applied several state-of-the-art tools our pipeline annotate these viruses, cluster them, taxonomically classify detect respective auxiliary metabolic genes. In total, identify characterize over 350,000 35,000 Our prophage is highly representative statistical results contains diverse set archaeal bacterial hosts which show wide environmental distribution. Conclusion Given particularly overlooked merit increased attention due vital implications microbiomes hosts, created Prophage-DB advance understanding through comprehensive characterization genomes. propose valuable advancing research, offering insights taxonomy, relationships, genes,

Язык: Английский

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Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL Escherichia coli

Antibiotics, Год журнала: 2025, Номер 14(1), С. 76 - 76

Опубликована: Янв. 13, 2025

Background/Objectives: Bacteriophage therapy represents a promising strategy to combat multidrug-resistant pathogens, such as Escherichia coli. In this study, we explored the effects of bacteriophage infection on an Extended Spectrum Beta-Lactamase (ESBL) positive E. coli isolate. Methods: We used next generation sequencing, proteomics and phenotypic screens investigate effect infections metabolism resistance phenotypes. Results: The led notable alterations in colony morphology, indicating profound changes bacterial metabolism. Proteomic analysis revealed significant shifts protein expression, with 65 proteins upregulated 246 downregulated post-infection. were involved various metabolic pathways, including nucleic acid, lipid metabolism, iron acquisition. treatment also increased membrane permeability. Altogether, these permeability may lead general reduction antibiotic resistance. Indeed, bacteriophage-infected exhibited sensitivity classes antibiotics, beta-lactams, fluoroquinolones, trimethoprim-sulfamethoxazole, aminoglycosides. Conclusions: Our findings highlight potential adjunct existing enhancing their efficacy against resistant strains.

Язык: Английский

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Combinatorial phenotypic landscape enables bacterial resistance to phage infection

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Success of phage therapies is limited by bacterial defenses against phages. While a large variety anti- defense mechanisms has been characterized, how expression these systems distributed across individual cells and their combined activities translate into protection from phages not studied. Using single-cell RNA sequencing, we profiled the transcriptomes ∼50,000 cultures human pathobiont, Bacteroides fragilis, infected with lytic bacteriophage. We quantified asynchronous progression infection in single reconstructed timeline, characterizing both host transcriptomic changes as unfolded. discovered subpopulation bacteria that remained uninfected determined heterogeneously expressed factors associated protection. Each cell's vulnerability to was defined combinatorial phase-variable multiple genetic loci, including capsular polysaccharide (CPS) biosynthesis pathways, restriction-modification (RM), previously uncharacterized operon likely encoding fimbrial genes. By acting together, anti-phage create phenotypic landscape where distinct protective combinations enable survival re-growth expressing phenotypes without acquiring additional mutations. The emerging model complementary action an isogenic population showcases potent role phase variation stochasticity defenses. Combinatorial states differential fragilis small number super-resistant evade need for

Язык: Английский

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Metagenomic mining reveals novel viral histones in dsDNA viruses

hLife, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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A new bacteriophage infecting Staphylococcus epidermidis with potential for removing biofilms by combination with chimeric lysin CHAPSH3b and vancomycin

mSphere, Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

ABSTRACT Staphylococcus epidermidis is the cause of serious skin and prosthetic joint infections despite being a common inhabitant human body surfaces. However, both rise in antibiotic resistance this species its ability to form biofilms are increasingly limiting available therapeutic options against these illnesses. In landscape, phage therapy stands out as an interesting alternative antibiotics. present study, we report isolation characterization novel virulent infecting S. ( IPLA-AICAT), which belongs Herelleviridae family. The estimated genome size virus 139,941 bp, sequence analysis demonstrated absence genes virulence factors. This infects high proportion (79%) clinically relevant strains exhibits antibiofilm activity. Moreover, combination with other antimicrobials, i.e., vancomycin lytic protein CHAPSH3b, further improved reduction surface-attached bacteria. Notably, IPLA-AICAT (10 9 PFU/mL) CHAPSH3b (8 µM), originally designed target aureus , was able reduce number viable cells by 3.06 log units 5-h-old biofilms. 24-h-old biofilms, also significant after 6 h treatment (2.06 units) 24 (2.52 units). These results confirm our previous data regarding potential phage-lysin mixtures staphylococcal IMPORTANCE one main causes device-associated mostly due stable attached tissues. Besides inherent antimicrobial tolerance microorganism becoming resistant standard-of-care To fight problem, option complement antibiotics recalcitrant infections. work describes new clinical that member family antimicrobials improve Together progress achieved development diagnostic tools, phages their derived proteins will bring us much closer landscape not so heavily reliant on combat bacterial pathogens.

Язык: Английский

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