Plant terpenoid biosynthetic network and its multiple layers of regulation

Progress in Lipid Research, Год журнала: 2024, Номер 95, С. 101287 - 101287

Опубликована: Июнь 19, 2024

Язык: Английский

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Specific sets of geranylgeranyl diphosphate synthases and phytoene synthases control the production of carotenoids and ABA in different tomato tissues

Physiologia Plantarum, Год журнала: 2025, Номер 177(1)

Опубликована: Янв. 1, 2025

Abstract Plant carotenoids are plastid‐synthesized isoprenoids with roles as photoprotectants, pigments, and precursors of bioactive molecules such the hormone abscisic acid (ABA). The first step carotenoid biosynthesis pathway is production phytoene from geranylgeranyl diphosphate (GGPP), catalyzed by synthase (PSY). GGPP produced plastidial synthases (GGPPS) channeled to direct interaction GGPPS PSY enzymes. Three plastid‐localized isoforms (referred SlG1‐3) three enzymes (PSY1‐3) present in tomato ( Solanum lycopersicum ). Our previous work showed that SlG1 PSY3 function together roots, whereas rest required aerial tissues. Here we generated analyzed combinations double mutants lacking PSY1 or PSY2 SlG2 SlG3 investigate contribution specific pairs ABA different tissues plant. Despite loss individual was found trigger compensatory mechanisms complicate interpretation results, results confirm a major role for providing housekeeping leaves, become most relevant when more active flowers breaker fruits, i.e., at onset ripening. We could also fruit pericarp dependent on activity than total levels size correlates accumulated ripe rather fruits.

Язык: Английский

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BoaBZR1.1 mediates brassinosteroid-induced carotenoid biosynthesis in Chinese kale

Horticulture Research, Год журнала: 2024, Номер 11(6)

Опубликована: Апрель 9, 2024

Brassinazole resistant 1 (BZR1), a brassinosteroid (BR) signaling component, plays pivotal role in regulating numerous specific developmental processes. Our study demonstrated that exogenous treatment with 2,4-epibrassinolide (EBR) significantly enhanced the accumulation of carotenoids and chlorophylls Chinese kale (Brassica oleracea var. alboglabra). The underlying mechanism was deciphered through yeast one-hybrid (Y1H) dual-luciferase (LUC) assays, whereby BoaBZR1.1 directly interacts promoters BoaCRTISO BoaPSY2, activating their expression. This effect further validated overexpression calli plants, both which exhibited increased carotenoid accumulation. Additionally, qPCR analysis unveiled upregulation chlorophyll biosynthetic genes T1 generation BoaBZR1.1-overexpressing plants. These findings underscored significance BoaBZR1.1-mediated BR suggested potential for enhancing nutritional quality genetic engineering BoaBZR1.1.

Язык: Английский

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Natural pigments derived from plants and microorganisms: classification, biosynthesis, and applications

Plant Biotechnology Journal, Год журнала: 2024, Номер unknown

Опубликована: Дек. 6, 2024

Summary Pigments, as coloured secondary metabolites, endow the world with a rich palette of colours. They primarily originate from plants and microorganisms play crucial roles in their survival adaptation processes. In this article, we categorize pigments based on chemical structure into flavonoids, carotenoids, pyrroles, quinones, azaphilones, melanins, betalains, flavins, others. We further meticulously describe colours, sources, biosynthetic pathways, including key enzymatic steps regulatory networks that control pigment production, both microorganisms. particular, highlight role transport proteins transcription factors fine‐tuning these pathways. Finally, introduce use practical production research, aiming to provide new insights directions for application compounds diverse fields, such agriculture, industry, medicine.

Язык: Английский

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Ghβ-LCY1 influences metabolism and photosynthetic in Gossypium hirsutum

Plant Science, Год журнала: 2025, Номер unknown, С. 112417 - 112417

Опубликована: Фев. 1, 2025

Язык: Английский

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Carotenoids: resources, knowledge, and emerging tools to advance apocarotenoid research

Plant Science, Год журнала: 2024, Номер 350, С. 112298 - 112298

Опубликована: Окт. 21, 2024

Язык: Английский

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GLKs directly regulate carotenoid biosynthesis via interacting with GBFs in plants

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Summary Carotenoids are vital photosynthetic pigments for plants. Golden2‐like transcription factors (GLKs) widely recognized as major regulators of Chl biosynthesis and chloroplast development. However, despite GLKs being subjected to intensive investigations, whether directly regulate carotenoid the molecular mechanisms by which transcriptionally activate their target genes remain unclear. Here, we report that in a G‐box binding factor (GBF)‐dependent manner Arabidopsis. Both vitro vivo studies reveal physically interact with GBFs phytoene synthase (PSY) , gene encoding rate‐limiting enzyme biosynthesis. While possess transactivation activity, they depend on bind motif modulate PSY expression. Loss impairs GLK function regulating Since is an enriched promoters GLK‐regulated genes, GLK–GBF regulatory module likely serves common mechanism underlying pigment Our findings uncover novel machinery biosynthesis, discover transcriptional regulation GLKs, divulge important coordinate synthesis

Язык: Английский

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Current insights into plant volatile organic compound biosynthesis

Current Opinion in Plant Biology, Год журнала: 2025, Номер 85, С. 102708 - 102708

Опубликована: Март 28, 2025

Язык: Английский

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Analysis of NUDIX enzymes across fungi reveals previously unrecognized diversity

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 24, 2025

Background The NUDIX superfamily encompasses highly diverse enzymes involved in a plethora of biological functions such as mRNA metabolism, DNA repair, and lipid peroxidation. These hydrolases are found all domains life show surprising versatility terms the substrates that they process. knowledge about diversity fungal proteins is fragmentary, being largely limited to small number characterized from yeasts. To address this gap systematically, we performed detailed analysis across 183 proteomes. Results Members six known families were present fungi particularly abundant Glomeromycota. Phylogenetic sequence clustering grouped 25 subfamilies, 13 which did not cluster with previously enzymes. newly identified subfamilies belong canonical family, structural comparison revealed typical fold α-β-α sandwich structure. Molecular docking suggested Ap3A Ap4A highest binding affinity, but their possible cellular roles remain unclear. We also evidence expression most genes encode these enzymes, suggesting physiological relevance. Conclusions Our offers comprehensive perspective on relationships potential guide experimental characterization functions.

Язык: Английский

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ORANGE family proteins: multifunctional chaperones shaping plant carotenoid level, plastid development, stress tolerance, and more

Molecular Horticulture, Год журнала: 2025, Номер 5(1)

Опубликована: Май 9, 2025

Abstract ORANGE (OR) family proteins are DnaJE1 molecular chaperones ubiquitous and highly conserved in all plant species, indicating their important roles growth development. OR have been found to exert multiple functions regulating carotenoid chlorophyll biosynthesis, plastid development, stress tolerance, with additional expected be discovered. As chaperones, directly influence the stability of target via holdase activity may perform other through unknown mechanisms. Exploration has uncovered novel mechanisms underlying core metabolism pathways expanded our understanding processes linked Continued investigation will not only reveal new but also provide pioneering tools for crop improvement. Thus, offer a distinctive opportunity comprehend modulating various metabolic developmental exemplify importance development adaptability. This review briefly details history proteins, highlights recent advancements myriad functions, discusses prospects this fascinating group towards generating innovative, more nutritious, resilient crops alongside agronomically traits.

Язык: Английский

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