Arabidopsis B‐box transcription factors BBX20‐22 promote UVR8 photoreceptor‐mediated UV‐B responses

The Plant Journal, Год журнала: 2022, Номер 111(2), С. 422 - 439

Опубликована: Май 12, 2022

Plants undergo photomorphogenic development in the presence of light. Photomorphogenesis is repressed by E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), which binds to substrates through their valine-proline (VP) motifs. The UV RESISTANCE LOCUS 8 (UVR8) photoreceptor senses UV-B and inhibits COP1 cooperative binding its own VP motif photosensing core COP1, thereby preventing substrates, including basic leucine zipper (bZIP) transcriptional regulator ELONGATED HYPOCOTYL 5 (HY5). As a key promoter visible light photomorphogenesis, HY5 requires coregulators for function. B-box family transcription factors BBX20-BBX22 were recently described as rate-limiting coactivators under red light, but role UVR8 signaling was unknown. Here we describe hypermorphic bbx21-3D mutant with enhanced carrying proline-to-leucine mutation at position 314 that impairs interaction regulation COP1. We show BBX21 BBX22 are UVR8-dependently stabilized after exposure, counteracted repressor induced HY5/BBX activity. bbx20 bbx21 bbx22 mutants impaired hypocotyl growth inhibition, photoprotective pigment accumulation expression several HY5-dependent genes continuous UV-B, immediate induction marker exposure remains surprisingly rather unaffected. conclude contribute activity subset responses, additional, presently unknown, functional early signaling.

Язык: Английский

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Dual Photo‐Responsive Diphenylacetylene Enables PET In‐Situ Upcycling with Reverse Enhanced UV‐Resistance and Strength

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(52)

Опубликована: Ноя. 8, 2023

A novel in situ chemical upcycling strategy for plastic waste is proposed by the customized diphenylacetylene monomer with dual photo-response. That is, reactive monomers are inserted into macromolecular chain of polyethylene terephthalate (PET) plastics/fibers through one-pot transesterification slight-depolymerization and re-polymerization. On one hand, group absorbs short-wave high-energy UV rays then releases long-wave low-energy harmless fluorescence. other UV-induced photo-crosslinking reaction among groups produces extended π-conjugated structure, resulting a red-shift (due to decreased HOMO-LUMO separation) absorption band locked crosslink points between PET chains. Therefore, increasing exposure time, upcycled plastics exhibit reverse enhanced resistance mechanical strength (superior original performance), instead serious UV-photodegradation damaged performance. This at oligomer-scale not only provides new idea traditional recycling, but also solves common problem gradual degradation polymer performance during use.

Язык: Английский

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WRKY Transcription Factors Modulate the Flavonoid Pathway of Rhododendron chrysanthum Pall. Under UV-B Stress

Plants, Год журнала: 2025, Номер 14(1), С. 133 - 133

Опубликована: Янв. 4, 2025

The depletion of the ozone layer has resulted in elevated ultraviolet-B (UV-B) radiation levels, posing a significant risk to terrestrial plant growth. Rhododendron chrysanthum Pall. (R. chrysanthum), adapted high-altitude and high-irradiation environments, developed unique adaptive mechanisms. This study exposed R. UV-B for two days, with an 8 h daily treatment, utilizing metabolomic transcriptomic analyses explore role WRKY transcription factors plant’s stress response their regulation flavonoid synthesis. decrease rETR Ik increase 1-qP. These chlorophyll fluorescence parameters indicate that impaired photosynthesis chrysanthum. Faced detrimental impact radiation, is capable mitigating its effects by modulating biosynthetic pathways adapt positively stress. revealed changes expression 113 flavonoid-related metabolites 42 associated genes, showing correlation these alterations. can influence key enzyme genes metabolic pathway, thereby affecting metabolite production. A theoretical reference investigating physiology provided this work, which also offers insights into responses alpine plants under adverse conditions.

Язык: Английский

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Downsizing in plants—UV light induces pronounced morphological changes in the absence of stress

PLANT PHYSIOLOGY, Год журнала: 2021, Номер 187(1), С. 378 - 395

Опубликована: Июнь 5, 2021

Abstract Ultraviolet (UV) light induces a stocky phenotype in many plant species. In this study, we investigate effect with regard to specific UV wavebands (UV-A or UV-B) and the cause for dwarfing. UV-A- UV-B-enrichment of growth both resulted smaller cucumber (Cucumis sativus L.) phenotype, exhibiting decreased stem petiole lengths leaf area (LA). Effects were larger plants grown UV-B- than UV-A-enriched light. light, decreases similar independent tissue age. presence UV-B radiation, stems petioles progressively shorter younger tissue. Also, under significantly reallocated photosynthates from shoot root also had thicker leaves LA. Our data therefore imply different morphological regulatory mechanisms UV-A radiation. There was no evidence stress UV-exposed plants, neither photosynthetic parameters, total chlorophyll content, accumulation damaged DNA (cyclobutane pyrimidine dimers). The abscisic acid content consistent non-stress conditions. Parameters such as antioxidant activity, adaxial epidermal flavonol foliar UV-absorbing pigment levels revealed successful acclimation plants. Thus, UV-induced dwarfing, which displayed phenotypes depending on wavelengths, occurred healthy implying adjustment part processes involving and/or photoreceptors.

Язык: Английский

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Transcription factors BBX11 and HY5 interdependently regulate the molecular and metabolic responses to UV-B

PLANT PHYSIOLOGY, Год журнала: 2022, Номер 189(4), С. 2467 - 2480

Опубликована: Апрель 27, 2022

UV-B radiation acts as a developmental cue and stress factor for plants, depending on dose. Activation of the transcription ELONGATED HYPOCOTYL 5 (HY5) in UV RESISTANCE LOCUS 8 (UVR8)-dependent manner leads to induction broad set genes under UV-B. However, underlying molecular mechanisms regulating this process are less understood. Here, we use molecular, biochemical, genetic, metabolomic tools identify B-BOX PROTEIN 11 (BBX11) component response Arabidopsis (Arabidopsis thaliana). BBX11 expression is induced by dose-dependent manner. Under low UV-B, regulates hypocotyl growth suppression, whereas it protects plants exposed high promoting accumulation photo-protective phenolics antioxidants, inducing DNA repair genes. Our genetic studies indicate that elongation partially dependent HY5. Overexpression can rescue sensitivity hy5, suggesting HY5-mediated tolerance BBX11. HY5 UV-B-mediated directly binding its promoter. reciprocally mRNA protein levels We report here role BBX11-HY5 feedback loop photomorphogenesis

Язык: Английский

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Photoreceptor-induced sinapate synthesis contributes to photoprotection in Arabidopsis

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 196(2), С. 1518 - 1533

Опубликована: Июнь 26, 2024

Abstract Plants must balance light capture for photosynthesis with protection from potentially harmful ultraviolet (UV) radiation. Photoprotection is mediated by concerted action of photoreceptors, but the underlying molecular mechanisms are not fully understood. In this study, we provide evidence that UV RESISTANCE LOCUS 8 (UVR8) UV-B, phytochrome red, and cryptochrome blue-light photoreceptors converge on induction FERULIC ACID 5-HYDROXYLASE 1 (FAH1) encodes a key enzyme in phenylpropanoid biosynthesis pathway, leading to accumulation UV-absorbing sinapate esters Arabidopsis (Arabidopsis thaliana). FAH1 depends basic leucine zipper transcription factors ELONGATED HYPOCOTYL 5 (HY5) HY5 HOMOLOG function downstream all 3 photoreceptors. Noticeably, mutants hyperactive UVR8 signaling rescue fah1 sensitivity. Targeted metabolite profiling suggests phenotypic due metabolites derived precursors synthesis, namely, coumaroyl glucose feruloyl glucose. Our genetic dissection pathway combined metabolomic physiological analyses show both flavonoids contribute photoprotection sinapates playing major role screening. findings indicate photoreceptor-mediated regulation subsequent “sunscreen” compounds protective mitigate damage, preserve photosynthetic performance, ensure plant survival under UV.

Язык: Английский

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Impact of Ultraviolet Radiation on Skin and Blood Melanin Traits in Xichou Black-Boned Chicken: A Transcriptomic and Metabolomic Study

Animals, Год журнала: 2025, Номер 15(2), С. 141 - 141

Опубликована: Янв. 8, 2025

This study investigated the effects of ultraviolet A (UVA) radiation on blackness traits Xichou Black-boned Chickens and their underlying molecular mechanisms through combined transcriptomic metabolomic analyses. total 240 one-day-old were randomly divided into four groups exposed to different durations UVA at an intensity 47 μW/cm2. Skin was measured 1, 22, 45 days age, blood pectoral skin samples collected for analysis. The results showed that exposure time 1 h per day μW/cm2 ideal enhancing chickens, while times exceeding 3 led a significant increase in mortality. Omics studies revealed mTOR pathway tryptophan metabolism are potentially involved biological processes by which affects Chickens, with FZD3 gene being candidate these effects. These findings provide theoretical foundations references understanding regulation chickens potential application production.

Язык: Английский

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Transcriptional survey of the light-induced anthocyanin pathway in non-GM purple tomatoes

Frontiers in Plant Physiology, Год журнала: 2025, Номер 2

Опубликована: Янв. 23, 2025

Anthocyanins are polyphenolic compounds with antioxidant capacity, free radical scavenging power, and signaling activities in animal pathogenesis-associated pathways, thus playing an important role as nutraceuticals. Tomato fruits do not usually contain anthocyanins because their biosynthesis is switched off these organs, but anthocyanin-enriched purple tomatoes have been produced recent years. The varieties obtained by breeding express a functional copy of the R2R3-MYB transcription factor AN2-like, necessary to start biosynthetic pathway, produce MYB-ATV repressor. combination traits allows accumulation tomatoes, strengthened under specific environmental factors such high light intensity or low temperatures. Light starts anthocyanin synthesis gradually extends its distribution on fruit exocarp. analyses carried out present study indicate that triggered HY5 control. However, process active mesocarp for absence bHLH AN1, MBW complex inducing late enzymes consequence insufficient expression gene AN2-like . This occurs since cannot be perceived tissues underneath skin solar shield accumulated exocarp activation regulatory loops controlling levels. shown genes involved production photoreceptors chain operating upstream pathway responsible activation.

Язык: Английский

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Tiller number: an essential criterion for developing high-yield and stress-resilient cereal crops

Archives of Agronomy and Soil Science, Год журнала: 2025, Номер 71(1), С. 1 - 21

Опубликована: Фев. 12, 2025

Язык: Английский

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Endophytic fungi as sources of cardiovascular drug leads

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 201 - 232

Опубликована: Янв. 1, 2025

Язык: Английский

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