The alternative splicing of ZmHsf23 regulates heat stress tolerance in maize DOI
Jing Wang, Nannan Song, Qianqian Qin

и другие.

Опубликована: Апрель 30, 2024

Summary Heat stress is one of the major threats to maize ( Zea mays ) production globally. shock transcription factors (HSFs) play vital roles in plant heat responses. However, molecular and genetic basis HSFs thermotolerance remain largely unknown. In this study, we reveal that alternative splicing Hsf23 modulates tolerance. produces two functional transcripts, full-length transcript Hsf23b heat-inducible Hsf23a . The transcripts differ by presence a cryptic mini exon , which spliced out Both were intensely expressed response stress. overexpression not enhanced tolerance, while loss-of-function mutations exhibited remarkably increased sensitivity Transcriptome analysis revealed activates broader heat-responsive genes than Hsf23a, modulate through different downstream targets. Furthermore, physically interacted with promotes Hsf23b-regulated expression sHSP genes. Together, our finding provides new insights into ZmHsf23 tolerance maize, presents an important candidate for improvement heat-tolerant varieties.

Язык: Английский

Transcription factor PgCDF2 enhances heat tolerance of Physalis grisea by activating heat shock transcription factors PgHSFA1 and PgHSFB3 DOI Open Access

Pengwei Jing,

Haimeng Zhang,

Ruxin Wang

и другие.

The Plant Journal, Год журнала: 2025, Номер 121(5)

Опубликована: Март 1, 2025

High temperature influence flower bud differentiation in Physalis grisea, resulting the production of deformed fruits and affects fruit yield quality. However, molecular mechanisms underlying response P. grisea to heat stress (HS) remain unclear. In this study, HS treatment dynamic transcriptome analysis identified PgCDF2-PgHSFA1/PgHSFB3 transcriptional regulatory module as playing a key role HS. Gene Ontology (GO) enrichment analysis, regulation prediction, weighted correlation network (WGCNA) (HS)-responsive data three genes, PgCDF2, PgHSFA1 PgHSFB3, components grisea. The expression levels PgHSFA1, PgHSFB3 were up-regulated following exposure Silencing resulted reduced tolerance altered reactive oxygen species Dual-luciferase assay Electrophoretic Mobility Shift Assay (EMSA) results indicate that PgCDF2 binds promoters activate their expression. inhibited also summary, under HS, enhances by activating PgHSFB3. This study clarifies providing theoretical basis for more in-depth response.

Язык: Английский

Процитировано

1
Pinellia ternata HD-Zip6 gene positive regulates heat stress tolerance in transgenic Arabidopsis by increasing ROS scavenging and NAC019 expression DOI Creative Commons
Mingfang Ji, Mengmeng Liu,

Xiaoyang Zheng

и другие.

Plant Stress, Год журнала: 2025, Номер unknown, С. 100806 - 100806

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
The 14-3-3 protein CaTFT7 interacts with transcription factor CaHDZ27 to positively regulate pepper immunity against Ralstonia solanacearum DOI Creative Commons
Shaoliang Mou, Xiaodan Chen,

Jiao Cai

и другие.

Horticulture Research, Год журнала: 2025, Номер 12(4)

Опубликована: Янв. 14, 2025

Abstract Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting plants in the Solanaceae family. In our previous study, CaHDZ27 was shown to act crucially pepper defense response R. solanacearum. However, molecular basis underlying function remains unexplored. this we demonstrate that post-translationally regulated 14-3-3 protein CaTFT7, which functions as positive regulator immunity against RT-qPCR analysis revealed CaTFT7 transcriptionally induced solanacearum infection. The data from virus-induced gene silencing positively affects immunity, further confirmed of CaTFT7-overexpressing Nicotiana benthamiana. interacted with CaHDZ27, thereby promoting stability and enhancing binding promoter cysteine-rich receptor-like kinase 5 (CaCRK5), above indicated enhanced promoted its ability activate shedding light on mechanisms resistance bacterial wilt.

Язык: Английский

Процитировано

0
Characterization and Functional Analysis of RhHsfA7, a Heat Stress Transcription Factor in Roses (Rosa hybrid ‘Samantha’) DOI Creative Commons
Yaqi Sun,

Sudan Li,

Xiang Wu

и другие.

Plants, Год журнала: 2025, Номер 14(8), С. 1155 - 1155

Опубликована: Апрель 8, 2025

Heat stress transcription factors (Hsfs) are crucial (TFs) in plants, playing pivotal roles responding to abiotic stresses. However, their specific functions regulating heat responses roses not yet fully elucidated. Here, we cloned an Hsf gene, RhHsfA7, from the rose variety Rosa hybrid ‘Samantha’. This gene contains a coding sequence (CDS) of 1086 bp, encoding 361 amino acids. The RhHsfA7 protein has molecular weight 41.21 kDa, isoelectric point 5.41, and no signal peptide or transmembrane structure. Phylogenetic analyses revealed that is most closely related AtHsfA7a, AtHsfA7b, AtHsfA6b Arabidopsis thaliana, phylogenetically closer Rosaceae species compared other species. possesses conserved domains, including oligomerization domain (OD), nuclear localization (NLS), DNA-binding (DBD), export (NES), as well HsfA subfamily-specific transcriptional activation (AHA). was localized nucleus exhibited activity. Expression analysis highly expressed roots leaves, its expression heat-specific. In through silencing transient overexpression experiments, discovered resulted sensitivity, whereas increased tolerance. Collectively, our findings suggest positively regulates tolerance roses.

Язык: Английский

Процитировано

0
CaMAPK1 Plays a Vital Role in the Regulation of Resistance to Ralstonia solanacearum Infection and Tolerance to Heat Stress DOI Creative Commons

Lanping Shi,

Wei Shi, Zhengkun Qiu

и другие.

Plants, Год журнала: 2024, Номер 13(13), С. 1775 - 1775

Опубликована: Июнь 27, 2024

As an important member of mitogen-activated protein kinase (MAPK) cascades, MAPKs play role in plant defense response against biotic and abiotic stresses; however, the involvement majority MAPK family members Ralstonia solanacearum heat stress (HS) remains poorly understood. In present study, CaMAPK1 was identified from genome pepper its function R. HS analyzed. The transcript accumulations activities native promoter were both significantly induced by inoculation, HS, application exogenous hormones, including SA, MeJA, ABA. Transient expression showed that can be targeted throughout whole cells Nicotiana benthamiana triggered chlorosis hypersensitive response-like cell death leaves, accompanied accumulation H2O2, up-regulations hormones- H2O2-associated marker genes. knock-down enhanced susceptibility to partially down-regulating H2O2-related genes impairing thermotolerance probably attenuating CaHSFA2 CaHSP70-1 transcripts. Taken together, our results revealed is regulated JA, ABA signaling coordinates responses infection pepper.

Язык: Английский

Процитировано

3
Genome-Wide Identification of Heat Shock Transcription Factor Family and Key Members Response Analysis to Heat Stress in Loquat DOI Creative Commons

Chaojun Deng,

Yongping Chen, Weilin Wei

и другие.

Horticulturae, Год журнала: 2024, Номер 10(11), С. 1195 - 1195

Опубликована: Ноя. 13, 2024

Eriobotrya japonica (loquat) is an evergreen fruit tree of the apple tribe in Rosaceae with high edible and medicinal value. The yield quality loquat fruits are significantly influenced by environmental stress, particularly heat stress during ripening. In this study, thirty EjHSFs were identified genome. Twenty-nine unevenly distributed across sixteen chromosomes, except Chr-6. A synteny analysis revealed that twenty-six EjHSF genes had undergone duplication events. found to be sync HSF apples while also diverging other species. phylogenetic could divided into three categories, including eighteen EjHSF-A, ten EjHSF-B, two EjHSF-C. members family showed homology those Malus domestica Gillenia trifoliate. promoter retrieved thirty-three CAREs functionally relevant connected expression HSFs. Among these, promoters twenty-three possessed at least one STRE element activated shock. Eleven these highly expressed tissues, namely EjHSF-B4a, EjHSF-A4a, EjHSF-A4d, EjHSF-C1a roots; EjHSF-B4b roots inflorescence; EjHSF-C1b stems EjHSF-A2a tissues; EjHSF-A8b four EjHSF-A4c, EjHSF-B1a, EjHSF-B2b six tissues. Moreover, fifteen differentially (eleven upregulated downregulated) under treatment color-changing period. them, EjHSF-A2b transcriptional abundance over 300 times after treatment; consistently displayed increasing trend time; EjHSF-B1a was downregulated. Hence, results suggest take part tissue development may involved fruit’s response. These findings enhance understanding EjHSFs’ role within loquats response its provide target for improvement.

Язык: Английский

Процитировано

2
The alternative splicing of ZmHsf23 regulates heat stress tolerance in maize DOI
Jing Wang, Nannan Song, Qianqian Qin

и другие.

Опубликована: Апрель 30, 2024

Summary Heat stress is one of the major threats to maize ( Zea mays ) production globally. shock transcription factors (HSFs) play vital roles in plant heat responses. However, molecular and genetic basis HSFs thermotolerance remain largely unknown. In this study, we reveal that alternative splicing Hsf23 modulates tolerance. produces two functional transcripts, full-length transcript Hsf23b heat-inducible Hsf23a . The transcripts differ by presence a cryptic mini exon , which spliced out Both were intensely expressed response stress. overexpression not enhanced tolerance, while loss-of-function mutations exhibited remarkably increased sensitivity Transcriptome analysis revealed activates broader heat-responsive genes than Hsf23a, modulate through different downstream targets. Furthermore, physically interacted with promotes Hsf23b-regulated expression sHSP genes. Together, our finding provides new insights into ZmHsf23 tolerance maize, presents an important candidate for improvement heat-tolerant varieties.

Язык: Английский

Процитировано

0