Опубликована: Июль 26, 2024
Язык: Английский
Bioactive Materials, Год журнала: 2025, Номер 50, С. 525 - 539
Опубликована: Апрель 29, 2025
Язык: Английский
Процитировано
0
Current Issues in Molecular Biology, Год журнала: 2023, Номер 46(1), С. 262 - 278
Опубликована: Дек. 29, 2023
Acute and chronic liver diseases cause significant morbidity mortality worldwide, affecting millions of people. Liver transplantation is the primary intervention method, replacing a non-functional with functional one. However, field faces challenges such as donor shortage, postoperative complications, immune rejection, ethical problems. Consequently, there an urgent need for alternative therapies that can complement traditional or serve method. In this review, we explore potential tissue engineering supplementary approach to transplantation, offering benefits patients severe dysfunctions.
Язык: Английский
Процитировано
9
Macromolecular Bioscience, Год журнала: 2024, Номер 24(6)
Опубликована: Янв. 24, 2024
Hydrogels are 3D networks swollen with water. They biocompatible, strong, and moldable emerging as a promising biomedical material for regenerative medicine tissue engineering to deliver therapeutic genes. The excellent natural extracellular matrix simulation properties of hydrogels enable them be co-cultured cells or enhance the expression viral non-viral vectors. Its biocompatibility, high strength, degradation performance also make action process carriers in tissues more ideal, making it an ideal material. It has been shown that hydrogel-based gene delivery technologies have potential play therapy-relevant roles organs such bone, cartilage, nerve, skin, reproductive organs, liver animal experiments preclinical trials. This paper reviews recent articles on explains manufacture, applications, developmental timeline, limitations, future directions techniques.
Язык: Английский
Процитировано
3
European Journal of Pharmaceutics and Biopharmaceutics, Год журнала: 2024, Номер 198, С. 114272 - 114272
Опубликована: Март 26, 2024
Язык: Английский
Процитировано
3
Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11
Опубликована: Фев. 23, 2023
The selection of a suitable matrix material is crucial for the development functional, biomimetic tissue and organ models. When these models are fabricated with 3D-bioprinting technology, requirements do not only include biological functionality physico-chemical properties, but also printability. In our work, we therefore present detailed study seven different bioinks focus on functional liver carcinoma model. Agarose, gelatin, collagen their blends were selected as materials based benefits 3D cell culture Drop-on-Demand (DoD) bioprinting. formulations characterized mechanical (G’ 10–350 Pa) rheological (viscosity 2–200 Pa*s) properties well albumin diffusivity (8–50 μm 2 /s). cellular behavior was exemplarily shown HepG2 cells by monitoring viability, proliferation morphology over 14 days, while printability microvalve DoD printer evaluated drop volume in flight (100–250 nl), camera imaging wetting microscopy effective diameter (700 µm more). We did observe negative effects viability or proliferation, which due to very low shear stresses inside nozzle (200–500 Pa). With method, could identify strengths weaknesses each material, resulting portfolio. By specifically selecting certain blends, migration possible interaction other can be directed indicated results experiments.
Язык: Английский
Процитировано
7
Current Opinion in Biomedical Engineering, Год журнала: 2024, Номер 30, С. 100526 - 100526
Опубликована: Фев. 13, 2024
Язык: Английский
Процитировано
2
Regenerative Biomaterials, Год журнала: 2024, Номер 11
Опубликована: Янв. 1, 2024
The decellularized extracellular matrix (dECM) has emerged as an effective medium for replicating the
Язык: Английский
Процитировано
2
Gels, Год журнала: 2023, Номер 9(6), С. 456 - 456
Опубликована: Июнь 1, 2023
Our study sought approaches for chronic liver failure (CLF) treatment and correction via cell-engineered constructs (CECs). They are built from biopolymer-based, microstructured, collagen-containing hydrogel (BMCG). We also strove to evaluate the functional activity of BMCG in regeneration. Materials Methods: Allogeneic cells (namely, hepatocytes; LC) together with mesenchymal multipotent stem bone marrow origin (MMSC BM; BMSCs) were adhered our compose implanted CECs. Thereafter, we investigated a model CLF rats receiving The had been provoked by long-term exposure carbon tetrachloride. comprised male Wistar (n = 120) randomized into 3 groups: Group 1 was control group saline hepatic parenchyma 40); 2 received only loaded CECs their livers 40). August 30) made up donor population LCs MMSC BM develop grafts animals 3. length 90 days. Results: shown affect both biochemical test values morphological parameters CLF. Conclusion: found BMCG-derived be operational active, regenerative potential. showed significant evidence forced regeneration that tended persist until end (day 90). phenomenon is reflected signs recovery day 30 after grafting (compared Groups 2), whereas structural features repair (necrosis prevention, missing formation vacuoles, degenerating LC number decrease, delay fibrotic transformation). Such implantation allogeneic might represent proper option correct treat CLF, as well maintain affected function patients needed.
Язык: Английский
Процитировано
4
Frontiers in Biomaterials Science, Год журнала: 2024, Номер 3
Опубликована: Фев. 6, 2024
The intricate nature of native cartilage, characterized by zonal variations in oxygen levels and ECM composition, poses a challenge for existing hydrogel-based tissue models. Consequently, these 3D models often present simplified renditions the tissue, failing to fully capture its heterogenous nature. combined effects hydrogel components, network properties, structural designs on cellular responses are overlooked. In this work, we aim establish more physiological cartilage through biofabrication photopolymerizable allylated-gelatin (GelAGE) Thiolated Heparin (HepSH) constructs with tailorable matrix stiffness customized architectures. This involves systematically studying how glycosaminoglycan together stiffness, availability within structures influence chondrogenic differentiation regional heterogeneity. A comprehensive library was successfully developed, encompassing GelAGE-HepSH hydrogels three distinct levels: 12, 55 121 kPa, unique geometries: spheres, discs, square lattices. soft hydrogels, localization differentiating cells observed be irregular, while stiff restricted overall secretion components. medium-stiff were found most applicable, supporting both uniform formation maintained shape fidelity. Three different architectures explored, where smaller spheres without gradients induced homogenous, hyaline formation. Conversely, fabrication larger (discs lattices) could utilized design Similarly, temporal drive interconnected deposition glycosaminoglycans (GAGs). Control samples GelAGE HepSH did not exhibit any notable changes chondrogenesis as function architectures, or concentrations. Overall, incorporation serve an amplifier biological from cues. conclusion, designed impose limitations induce zone-specific alignment. systematic study biofabricated contributes development physiologically relevant further enhancing our understanding engineering.
Язык: Английский
Процитировано
1
Advanced Science, Год журнала: 2024, Номер unknown
Опубликована: Дек. 24, 2024
Human induced pluripotent stem cell derived hepatocytes (hiPSC-heps) hold promising value for acute liver failure (ALF) treatment, while their therapeutic efficacy is usually limited by low bioactivity and untargeted in vivo accumulation. Here, inspired vascularity supporting cellular architectures the tissues organs, a novel vascularized hiPSC-heps spheroid based on microfluidic microcapsules presented repair via orthotopic transplantation. The are comprised of aqueous cores that facilitate hiPSC-hep aggregating into spheroids, hybrid hydrogel shells sodium alginate hyaluronic acid methacryloyl (HAMA). By selectively degrading alginate, imparted with porous HAMA shells, which not only allowed human umbilical vein endothelial cells (HUVECs) to attach form networks, but also facilitated communication between HUVECs hiPSC-heps. specific spatial distributions these spheroids can provide nutrition support, promote hepatic functions, avoid immune attacks. Based features, it illustrated failing more effectively, indicating practical values clinical repair.
Язык: Английский
Процитировано
1