Per- and Polyfluoroalkyl Substances Suppress Macrophage Alternative Activation to Disrupt Hepatic Lipid Metabolism DOI

Lijuan You,

Xiaohong Wang,

Yuan Zhi

и другие.

Chemical Research in Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Май 29, 2025

Per- and polyfluoroalkyl substances (PFAS) are pervasive environmental pollutants with diverse toxic effects (e.g., hepatotoxicity metabolism disorder). Macrophages played a key role in metabolic response; however, the effect of macrophage on PFAS-induced toxicity underlying mechanisms remain poorly understood. In this study, we constructed high-content cell model by utilizing activation differentiation human THP-1 monocytes into alternative macrophages, enabling rapid quantitative screening numerous PFAS. We applied to screen 10 PFASs identified that PFOA PFUnDA significantly suppressed macrophages disrupting PPAR signaling pathway. Oral exposure WT mice also impaired liver induced hepatocyte hypertrophy, dysfunction, systemic lipid disorders. Moreover, macrophage-specific knockout PPARγ exacerbated PFUnDA-induced suppression subsequent hepatotoxicity. Activation balance between PPARα may be critical factor affect macrophage. These findings highlight immunometabolism regulatory humans.

Язык: Английский

PFAS: The Journey from Wonder Chemicals to Environmental Nightmares and the Search for Solutions DOI Creative Commons

Duwage C. Perera,

Jay N. Meegoda

Applied Sciences, Год журнала: 2024, Номер 14(19), С. 8611 - 8611

Опубликована: Сен. 24, 2024

Per- and polyfluoroalkyl substances (PFAS) are diverse synthetic chemicals manufactured over seven decades. It is an aliphatic molecule with a basic hydrophobic structure of carbon fluorine linked to hydrophilic end group. Due their physicochemical properties associated the unique structure, PFAS has been used in wide variety applications including aqueous film-forming foams (AFFF), paper, carpets, non-stick cookware, etc. as they make products resistant water, heat, stains. These molecules have drawn great attention recently for properties, high stability low degradability, so-called “Forever Chemicals”. strongest carbon-fluorine bond which makes them persistent environment. Hence it contaminates natural resources endangers public health. This review discusses discovery, development, evolution from wonder chemical era nightmare era, exposure its impacts on human health environment, current remediation techniques, future trends related products. The primary objective this identify knowledge gaps contamination, methods, possible alternatives.

Язык: Английский

Процитировано

4
Exposure to specific polyfluoroalkyl chemicals is associated with cardiovascular disease in US adults: a population-based study DOI Creative Commons
Wenwen Xiao,

Guojin Jian,

Fei Ma

и другие.

Frontiers in Cardiovascular Medicine, Год журнала: 2025, Номер 11

Опубликована: Янв. 9, 2025

Polyfluoroalkyl chemicals (PFCs) present potential health risks due to their persistence and bioaccumulation. However, there is currently insufficient evidence regarding impact on cardiovascular disease (CVD). Consequently, it imperative investigate the correlation between PFCs CVD. The data was collected from National Health Nutrition Examination Survey in 2005-2012. Logistic regression models were employed assess association single PFC Generalized additive model (GAM) used for evaluating nonlinear relationships. Subgroup analyses conducted explore interaction effects. Bayesian kernel machine (BKMR) weighted quantile sum (WQS) evaluate joint effect of exposures In logistic regression, PFDE, MPAH, PFUA positively associated with GAM, a significant relationship MPAH analysis revealed gender race effects correlated CVD males but show no difference females. PFDE among non-Hispanic white individual. results BKMR indicated that mixed increased initially then weakened, showing an overall positive trend. WQS suggested PFDO contributed most effect. Our study showed serum levels PUFA found interact relation A general exists exposure CVD, being contributory PFC. provided important probing laid foundation further mechanism research.

Язык: Английский

Процитировано

0
PFOS and Its Substitute OBS Cause Endothelial Dysfunction to Promote Atherogenesis in ApoE–/– Mice DOI Creative Commons
Boxiang Zhang, Qing Li, Wensheng Wang

и другие.

Environment & Health, Год журнала: 2025, Номер 3(5), С. 526 - 538

Опубликована: Фев. 11, 2025

Perfluorooctanesulfonate (PFOS), an emerging contaminant with widespread concern, has been associated the pathogenesis of atherosclerosis (AS). As a substitute for PFOS, sodium p-perfluorous nonenoxybenzenesulfonate (OBS) is extensively utilized in various applications and detected human blood. However, its potential health risk AS remain unclear. In this study, we investigated comparative impacts PFOS OBS on endothelial dysfunction atherogenesis. vivo Apolipoprotein E knockout (ApoE-/-) mice were exposed to 0.4 or 4 mg/L PFOS/OBS 12 weeks. We found that dyslipidemia developed more rapidly OBS-exposed than PFOS-exposed mice. exhibited higher enrichment capacity both blood aortic tissues OBS. Remarkably, induced pronounced inflammatory response caused significant disruption barrier aorta ApoE-/- compared PFOS. vitro experiments showed OBS, at same exposure concentrations durations as (0.1-20 μmol/L, 48 h), effectively inhibited cell viability umbilical vein cells (HUVECs), levels lactate dehydrogenase (LDH) release, enhanced adhesion between HUVECs monocytes. Both activate NF-κB signaling pathway upregulate expression factors. Notably, use but not was shown disrupt junctions increase permeability by activating MAPK/ERK pathway. Our findings suggest may lead have greater impact presenting risks cardiovascular diseases.

Язык: Английский

Процитировано

0
ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer DOI Creative Commons

Xiaorui Chen,

Xueying Tan,

Zong-liang Zhang

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 31, 2025

Background Studies have shown that ESCRT genes affect cell aging, immune environment, and tumors. BRCA was used to explore its effects on tumor prognosis therapy. Methods The data sets of Cancer Genome Atlas (TCGA), Genome-Tissue Expression Plan (GTEX), Human Protein Mapping (HPA), Gene Omnibus (GEO), Clinical Proteomic Tumor Analysis Consortium (CPTAC), R software package, bioinformatics methods were mine the potential carcinogenic ESCRT, including expression level, prognostic value, value in various types tissues, function family further verified breast cancer. Results showed significant differential cancers, such as bladder urothelial carcinoma liver, cervical, renal, esophageal, head, neck cancers ( P < 0.05). Abnormal is associated with poor adrenocortical carcinoma, cancer, cervical cancer level significantly infiltration modulation stromal/immune score (all Enrichment analysis immune-related functions transport signaling pathways cells. Moreover, serves an early diagnostic marker for several tumors prognosis. This confirms most immune-infiltrating cells pan-carcinomas. Taken together, these studies highlight importance gene VPS37D initiation, progression, response. Conclusion study highlights ESCRT’s detection via pan-cancer analysis, showing variations between normal role progression through microenvironment, specificity sensitivity detection. gene, variation predicts patient related suggesting a novel biomarker diagnosis assessment.

Язык: Английский

Процитировано

0
Effects of co-exposure to microplastics and perfluorooctanoic acid on the Caco-2 cells DOI

Xingyu Zou,

Min‐Hsiung Pan, Yue Liu

и другие.

Toxicology, Год журнала: 2025, Номер 515, С. 154152 - 154152

Опубликована: Апрель 11, 2025

Язык: Английский

Процитировано

0
Per- and Polyfluoroalkyl Substances Suppress Macrophage Alternative Activation to Disrupt Hepatic Lipid Metabolism DOI

Lijuan You,

Xiaohong Wang,

Yuan Zhi

и другие.

Chemical Research in Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Май 29, 2025

Per- and polyfluoroalkyl substances (PFAS) are pervasive environmental pollutants with diverse toxic effects (e.g., hepatotoxicity metabolism disorder). Macrophages played a key role in metabolic response; however, the effect of macrophage on PFAS-induced toxicity underlying mechanisms remain poorly understood. In this study, we constructed high-content cell model by utilizing activation differentiation human THP-1 monocytes into alternative macrophages, enabling rapid quantitative screening numerous PFAS. We applied to screen 10 PFASs identified that PFOA PFUnDA significantly suppressed macrophages disrupting PPAR signaling pathway. Oral exposure WT mice also impaired liver induced hepatocyte hypertrophy, dysfunction, systemic lipid disorders. Moreover, macrophage-specific knockout PPARγ exacerbated PFUnDA-induced suppression subsequent hepatotoxicity. Activation balance between PPARα may be critical factor affect macrophage. These findings highlight immunometabolism regulatory humans.

Язык: Английский

Процитировано

0