Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 7, 2024

Hepatocellular carcinoma (HCC) is a malignant tumor of the digestive system and one leading causes cancer mortality worldwide. So far, various genetic mutations have been observed in hepatocellular related with tumorigenesis due to genomic instability. However, clinical characteristic therapeutic value HCC were extent unknown. In present study, by analyzing gene mutation landscape tissues from TCGA database, we demonstrated that TTN (titin) was high-frequency HCC, relevant poor prognosis HCC. had no relationship immune signature but linked change drug sensitivity GSK1904529A, nilotinib, 5-Fluorouracil (5-FU) sapitinib. Furthermore, through performing TIDE algorithm, CCK-8 assays, colony formation cell sphere-forming experiment, vivo transplantation experiment biological experiments investigated association between sensitivity. We found led increased stability protein, which depletion results decrease intracellular ferrous ion content, inhibiting ferroptosis pathway, significantly reduced 5-FU vitro vivo. These findings cause reduce 5-FU, reducing treatment efficacy. conclusion, our studies provided initial evidences can sever as biomarker for predicting prognosis, signature, molecular targets therapy It provides new idea solving resistance response treatment. Background: Methods: database Results: The research identified And leads decreasing levels, pathway. Conclusions: high frequency genes promotes protein stability, demonstrate weaken providing target address therapy.

Язык: Английский