Environmental transcriptomics under heat stress: Can environmental RNA reveal changes in gene expression of aquatic organisms?

Molecular Ecology, Год журнала: 2023, Номер unknown

Опубликована: Окт. 4, 2023

Abstract To safeguard biodiversity in a changing climate, taxonomic information about species turnover and insights into the health of organisms are required. Environmental DNA approaches increasingly used for identification, but cannot provide functional insights. Transcriptomic methods reveal physiological states macroorganisms, currently species‐specific require tissue sampling or animal sacrifice, making community‐wide assessments challenging. Here, we test whether broad (expression level transcribed genes) can be harnessed from environmental RNA (eRNA), which includes extra‐organismal macroorganisms along with whole microorganisms. We exposed Daphnia pulex as well phytoplankton prey microorganism colonizers to control (20°C) heat stress (28°C) conditions 7 days. sequenced eRNA tank water (after complete removal ) tissue, enabling comparisons organismal RNA‐based gene expression profiles. Both types detected similar responses . Using eRNA, identified 32 genes differentially expressed following stress. Of these, 17 were also exhibited levels relative RNA. In addition response, consisting distinct profiles 121 across eight taxa. Our study demonstrates that transcriptomics based on noninvasively changes, potential implications biomonitoring trophic chain.

Язык: Английский

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Are Mediterranean marine threatened species at high risk by climate change?

Global Change Biology, Год журнала: 2022, Номер 29(7), С. 1809 - 1821

Опубликована: Дек. 30, 2022

Abstract Rapid anthropogenic climate change is driving threatened biodiversity one step closer to extinction. Effects on native are determined by an interplay between species' exposure and their specific ecological life‐history characteristics that render them even more susceptible. Impacts have already been reported, however, a systematic risk evaluation of marine populations lacking. Here, we employ trait‐based approach assess the 90 Mediterranean species change, combining increased sea temperature intrinsic vulnerability. One‐quarter Sea predicted be under elevated levels risk, with various traits identified as key vulnerability traits. High‐risk taxa including turtles, mammals, Anthozoa Chondrichthyes highlighted. Climate hotspots distributed along Western Mediterranean, Alboran, Aegean, Adriatic Seas. At each ecoregion, 21%–31% high risk. All protected areas host 90% having minimum 4 up 19 making objective climate‐smart conservation strategy crucial task for immediate planning action. Our findings aspire offer new insights systematic, spatially strategic prioritization vulnerable life in face accelerating change.

Язык: Английский

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Niche-Specific Restructuring of Bacterial Communities Associated with Submerged Macrophyte under Ammonium Stress

Applied and Environmental Microbiology, Год журнала: 2023, Номер 89(7)

Опубликована: Июль 5, 2023

Increasing anthropogenic input of ammonium is accelerating the decline submerged macrophytes in aquatic ecosystems. Finding efficient ways to release from stress crucial maintain their ecological benefits.

Язык: Английский

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TP or Not TP? Successful Comparison of Two Independent Methods Validates Total Phosphorus Inference for Long-Term Eutrophication Studies

Environmental Science & Technology, Год журнала: 2024, Номер 58(17), С. 7425 - 7432

Опубликована: Апрель 19, 2024

Validating paleo total phosphorus (TP) inference methods over long time scales is essential for understanding historic changes in lake P supply and the processes leading up to present-day global eutrophication crisis. Monitored water TP series have enabled us identify drivers of recent decades. However, longer scales, lack reliable means our speculative. Validation reconstruction, therefore, remains "ultimate aim" studies. Here, we present first critical comparison two fully independent approaches: well-established diatom method (DI-TP) a recently developed sediment geochemical (SI-TP). Using records from small eutrophic U.K. (Crose Mere), find statistically significant agreement between inferred with greater than 60% shared variance. Both show identical timings, 19th century acceleration concentration subsequent declines following peak 1930. This establishes validity long-term time. With this, can now test assumptions paradigms that underpin catchment sources pathways scales.

Язык: Английский

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From land to deep sea: A continuum of cumulative human impacts on marine habitats in Atlantic Canada

Ecosphere, Год журнала: 2024, Номер 15(9)

Опубликована: Сен. 1, 2024

Abstract Effective management and mitigation of multiple human impacts on marine ecosystems require accurate knowledge the spatial patterns activities their overlap with vulnerable habitats. Cumulative impact (CI) mapping combines information intensity extent habitats vulnerabilities to those stressors into an intuitive relative CI score that can inform planning processes ecosystem‐based management. Here, we mapped potential CIs 45 from five sectors (climate change, land‐based, marine‐based, coastal, commercial fishing) 21 in Atlantic Canada's Scotian Shelf bioregion. We applied uncertainty sensitivity analysis assess robustness results identify hot cold spots CIs. Nearly entire bioregion experiences activities, high were frequently associated stressors. varied widely across habitats: scores >30 m deep dominated by climate change fishing, while nearshore influenced a much wider range all sectors. When standardized area, coastal had among highest scores, highlighting these despite relatively small emphasizing importance multisector approach when managing ecosystems. Robust (i.e., areas insensitive alternative modeling assumptions simulated data quality issues) occurred mostly where high‐intensity overlapped highly biogenic In contrast, robust offshore. Overall, our emphasize need consider protection demonstrates that, many areas, targeting only one activity will be insufficient reduce overall impact. The map useful highlight impacts, provide for ecological indicator development, establish baseline current state use

Язык: Английский

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Can the establishment of a protected area improve the lacustrine environment? A case study of Lake Chaohu, China

Journal of Environmental Management, Год журнала: 2023, Номер 342, С. 118152 - 118152

Опубликована: Май 17, 2023

Язык: Английский

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Toxic effect of warm temperatures and antidepressants on the righting behavior of the freshwater snail ( Physa gyrina ) from Pennsylvania, USA

Toxicological & Environmental Chemistry Reviews, Год журнала: 2025, Номер 107(1), С. 207 - 222

Опубликована: Янв. 2, 2025

Язык: Английский

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Varying Thermal Dependence of Life History Traits Predicts Responses to Environmental Change in Aquatic Amphibian Larvae

Freshwater Biology, Год журнала: 2025, Номер 70(2)

Опубликована: Фев. 1, 2025

ABSTRACT Many organisms respond to environmental changes through plastic modifications of their phenotypes. While phenotypic plasticity has been extensively studied in response single factors laboratory settings, nature face the combined effects diverse biotic and abiotic stressors. These multistressor complicate predictions about impact on population dynamics. One approach improving predictive accuracy is identify common mechanistic links variations life‐history traits. In this study, we examined how stressors influence variation, using based differing thermal sensitivities differentiation growth rates aquatic amphibian larvae subsequent behaviour terrestrial juveniles. Using a mesocosm experiment, exposed two sympatric newt species, Ichthyosaura alpestris Lissotriton vulgaris , combinations factor (the presence heterospecifics) an (simulated drying). Our results revealed that simulated drying increased availability high water temperatures, thereby shortening larval period as predicted by sensitivity rates. The heterospecifics, not regime, size at emergence larger I. consistent with relatively low carried over juveniles, affecting covered distance both taxa, while heterospecifics influenced maximum voluntary speed during exploratory This suggests additive subsets behavioural traits combine individual level, depending species identity. Future research should focus interplay between ecologically‐realistic conditions interactions within pond assemblages understand

Язык: Английский

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Electrochemical phosphorus recovery from wastewater: a critical analysis of mechanisms, process optimization, and future pathways toward industrial application

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162468 - 162468

Опубликована: Апрель 1, 2025

Язык: Английский

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Nutrient inversion but not warming drive changes in periphyton biomass and composition in shallow lake mesocosms

Limnology and Oceanography, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract Periphyton plays an important functional role in shallow lakes and is sensitive to variation nutrient availability global warming. Understanding the responses of periphyton changes contrasting warming scenarios can contribute development more efficient techniques for restoration a future warmer world. We analyzed response early eutrophication oligotrophication under three different explored associated environmental drivers 24 lake mesocosms. These were initially set up 2003 with two treatments (with or without addition) temperatures (ambient, A2 IPCC scenario increased by 50%) following full factorial design. In 2023, treatment was inverted, starting addition low mesocosms (nutrient increase) stopping decrease) remaining. Artificial plants placed each mesocosm allow develop 21 d, both before after inversion treatments. found marked biomass composition inversion, but no Nutrient decrease induced lower compositional at species group levels. increase had effect on accrual altered composition. Our results suggest that nitrate crucial factor driving cessation addition, while combination variables explained treatment.

Язык: Английский

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