Diversity and Global Distribution of Viruses of the Western Honey Bee, Apis mellifera

Insects, Год журнала: 2020, Номер 11(4), С. 239 - 239

Опубликована: Апрель 10, 2020

In the past centuries, viruses have benefited from globalization to spread across globe, infecting new host species and populations. A growing number of been documented in western honey bee, Apis mellifera. Several these contribute significantly bee colony losses. This review synthetizes knowledge diversity distribution honey-bee-infecting viruses, including recent data high-throughput sequencing (HTS). After presenting their corresponding symptoms, we surveyed scientific literature for prevalence pathogens globe. The geographical shows that most prevalent (deformed wing virus, sacbrood black queen cell virus acute paralysis complex) are also widely distributed. We discuss ecological drivers influence worldwide Besides natural transmission routes resulting temporal dynamics, global trade contributes dissemination. As evidence often multihost pathogens, is a risk both beekeeping industry pollination services provided by managed wild pollinators.

Язык: Английский

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Shift in virus composition in honeybees ( Apis mellifera ) following worldwide invasion by the parasitic mite and virus vector Varroa destructor

Royal Society Open Science, Год журнала: 2024, Номер 11(1)

Опубликована: Янв. 1, 2024

Invasive vectors can induce dramatic changes in disease epidemiology. While viral emergence following geographical range expansion of a vector is well known, the influence have at level host's pathobiome less understood. Taking advantage formerly heterogeneous spatial distribution ectoparasitic mite Varroa destructor that acts as potent virus among honeybees Apis mellifera , we investigated impact its recent global spread on community retrospective study historical samples. We hypothesized has had an effect epidemiology several bee viruses, potentially altering their transmissibility and/or virulence, and consequently prevalence, abundance, or both. To test this, quantified prevalence loads 14 viruses from honeybee samples collected mite-free mite-infested populations four independent regions. The presence dramatically increased load deformed wing virus, cause unsustainably high colony losses. In addition, other became more prevalent were found higher areas, including not known to be actively varroa-transmitted, but which may increase opportunistically varroa-parasitized bees.

Язык: Английский

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Bee Viruses: Routes of Infection in Hymenoptera

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Май 28, 2020

Numerous studies have recently reported on the discovery of bee viruses in different arthropod species and their possible transmission routes, vastly increasing our understanding these distribution. Here, we review current literature recent advances viruses, both presence Apis non-Apis previously unknown viruses. The natural will be discussed among other insects. Finally, research potential vivo (host organisms) vitro (cell lines) serial passages is discussed, from perspective host-virus landscape changes routes for emerging virus infections.

Язык: Английский

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Abundant and Diverse RNA Viruses in Insects Revealed by RNA-Seq Analysis: Ecological and Evolutionary Implications

mSystems, Год журнала: 2020, Номер 5(4)

Опубликована: Июль 6, 2020

Increasing data indicate that insects serve as major reservoirs and vectors of viruses, which account for the continuously increasing ecological burden infectious disease outbreaks. Uncovering hidden diversity viruses in will further understanding evolutionary perspectives emergence insect-associated virus diseases. In this study, we queried transcriptome sequencing (RNA-Seq) from more than 600 species across 32 insect orders dwelling different habitats recovered 1,213 RNA were recapitulated 40 families, 2 unclassified genera, many unspecified viral groups. These novel included well-known within Flaviviridae, Picornavirales, Bunyavirales, Mononegavirales, Nidovirales, Reoviridae, Negevirus More appeared to form clusters previously described taxa or could be resolved paraphyletic, including first astrovirus identified insects, sufficiently divergent warrant establishment new genera families. Additionally, some closely related recognized plant-, fungus-, vertebrate-specific species, implying importance relationships between behavior spread. Comparative genome analyses also revealed high genomic variability with respect flexible gene pool architecture these newly evidence reshuffling discovered Dicistroviridae The reflecting genetically ecologically diverse populations greatly expand our nature highlight biodiversity remains largely unexplored.IMPORTANCE Insects comprise largest proportion animals on earth are frequently implicated transmission vector-borne However, considerable attention has been paid phytophagous hematophagous results provide insufficient biased information about insects. Here, have delivered compelling exceptional abundance genetic a wide range Novel found cover categories formed taxa, dramatically broadening known characterized exhibited levels plasticity size, open reading frame (ORF) number, intergenic structure, rearrangement segmentation. This work provides comprehensive insight into origin, spread, evolution viruses. Of course, large-scale virome project involving organisms would more-detailed infections

Язык: Английский

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DWV-A Lethal to Honey Bees (Apis mellifera): A Colony Level Survey of DWV Variants (A, B, and C) in England, Wales, and 32 States across the US

Viruses, Год журнала: 2019, Номер 11(5), С. 426 - 426

Опубликована: Май 9, 2019

The strong association between Varroa destructor, deformed wing virus (DWV), and high overwintering colony losses (OCL) of honey bees is well established. Three DWV master variants (DWV-A, -B, -C) have been described, their role in mortality remains an open question. Therefore, the aim this study to investigate seasonal prevalence, viral load, changing distribution three within bee colonies from England, Wales, 32 states across United States. Here, we report that 2016, DWV-B was prevalent (100%, n = 249) dominant (95%) England compared US. (56%, 217 23%, respectively), where DWV-A (83%, 217) (63%). DWV-C regularly detected low loads (<1 × 107 genome equivalents per bee) at lower prevalence (58% 203, 14% States, 124) -B. dominance Wales coincided with OCL (6%). Meanwhile, a 60% loss reported by participating U.S. beekeepers. In (89%, 18) load were significantly (p 0.002) higher (1 10 8–1 1011) died when surviving (49% (n 27), 1 106–1 1010). had <1 1010. However, routinely (>1 1010) all sample locations, providing further supporting evidence exhibiting increased virulence over level.

Язык: Английский

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The final frontier: ecological and evolutionary dynamics of a global parasite invasion

Biology Letters, Год журнала: 2023, Номер 19(5)

Опубликована: Май 1, 2023

Studying rapid biological changes accompanying the introduction of alien organisms into native ecosystems can provide insights fundamental ecological and evolutionary theory. While powerful, this quasi-experimental approach is difficult to implement because timing invasions their consequences are hard predict, meaning that baseline pre-invasion data often missing. Exceptionally, eventual arrival Varroa destructor (hereafter Varroa) in Australia has been predicted for decades. a major driver honeybee declines worldwide, particularly as vectors diverse RNA viruses. The detection 2022 at over hundred sites poses risk further spread across continent. At same time, careful study Varroa's spread, if it does become established, wealth information fill knowledge gaps about its effects worldwide. This includes how affects populations pollination. Even more generally, invasion serve model evolution, virology interactions between parasite, host other organisms.

Язык: Английский

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Diversity of RNA viruses in agricultural insects

Computational and Structural Biotechnology Journal, Год журнала: 2023, Номер 21, С. 4312 - 4321

Опубликована: Янв. 1, 2023

Язык: Английский

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Honey microbiota, methods for determining the microbiological composition and the antimicrobial effect of honey – A review

Food Chemistry X, Год журнала: 2024, Номер 23, С. 101524 - 101524

Опубликована: Июнь 5, 2024

Honey is a natural product used since ancient times due to its taste, aroma, and therapeutic properties (antibacterial, antiviral, anti-inflammatory, antioxidant activity). The purpose of this review present the species microorganisms that can survive in honey effect they have on bees consumers. techniques for identifying are also described study. contains bacteria, yeasts, molds, viruses, some them may beneficial humans. antimicrobial acidity high viscosity, sugar concentration, low water content, presence hydrogen peroxide non-peroxidase components, particularly methylglyoxal (MGO), phenolic acids, flavonoids, proteins, peptides, glycopeptides. has antibacterial action (it effectiveness against e.g. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter, etc.), antifungal (effectiveness Candida spp., Aspergillus Fusarium Rhizopus Penicillium spp.), antiviral SARS-CoV-2, Herpes simplex virus type 1, Influenza A B, Varicella zoster virus), antiparasitic Plasmodium berghei, Giardia Trichomonas, Toxoplasma gondii) demonstrated by numerous studies comprised discussed review.

Язык: Английский

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Influence of Hyperthermia Treatment on Varroa Infestation, Viral Infections, and Honey Bee Health in Beehives

Insects, Год журнала: 2025, Номер 16(2), С. 168 - 168

Опубликована: Фев. 5, 2025

The mite Varroa destructor is widely acknowledged as the most destructive threat to honey bee (Apis mellifera) colonies on a global scale. infestations in are intricately linked with viral infections, collaboratively leading diminished populations and accelerated colony losses. Extensive research has firmly established correlation between varroa mites viruses, underscoring mite’s efficiency spreading viruses among bees colonies. effective control of expected result decrease infections within Research suggests that thermal treatments (hyperthermia) present viable approach combat mites, studies demonstrating role heat stress reducing affected bees. This article examines extant literature surrounding utilization hyperthermia potential method ameliorate adverse impacts their associated It also outlines characteristics these stressors. Diverse devices can be used for subjecting treatment, targeting both outside brood cells. application treatments, typically ranging 40 42 °C 1.5–3 h, reduce shown promise. Notably, precise effectiveness treatment comparison alternative measures remains uncertain available literature. deleterious repercussions this mechanism immature mature evaluated. Concurrently, detrimental implications prolonged durations discussed. Regarding impact them negatively by either or inducing production shock proteins possess antiviral properties. Various factors identified influential efficacy colonies, including device type duration, necessitating further empirical investigations. Additionally, highlights existing gaps knowledge provides insights into prospective directions concerning method.

Язык: Английский

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Direct transmission by injection affects competition among RNA viruses in honeybees

Proceedings of the Royal Society B Biological Sciences, Год журнала: 2019, Номер 286(1895), С. 20182452 - 20182452

Опубликована: Янв. 30, 2019

The arrival of the ectoparasitic mite Varroa destructor on western honeybee Apis mellifera saw a change in diversity and prevalence RNA viruses. One virus particular, deformed wing (DWV) has become closely associated with V. , leading many to conclude that affected viral virulence by changing mode transmission. While DWV is normally transmitted via feeding faeces, transmits viruses direct injection. This could have resulted higher causing increased damage bees. Here we test effect transmission composition levels absence . We find rapid increase two viruses, sacbrood (SBV) black queen cell (BQCV) after injection extracts into pupae. In pupae injected high extracted from symptomatic adult bees, rapidly decline presence SBV BQCV. Further, observe mortality when BQCV, whereas injecting results near 100% survival. Our suggest different explanation for observed association between DWV. Instead an virulence, hypothesize inoculation, such as mediated vector, quickly eliminates most virulent resulting less

Язык: Английский

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