Cell-type-specific expression of aDCYTBortholog enables the choanoflagellateSalpingoeca rosettato utilize ferric colloids

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 27, 2024

Abstract Marine microeukaryotes have evolved diverse cellular features that link their life histories to surrounding environments. How those dynamic intersect with the ecological functions of remains a frontier understand roles in essential biogeochemical cycles 1,2 . Choanoflagellates, phagotrophs cycle nutrients through filter feeding, provide models explore this intersection, for many choanoflagellate species transition between history stages by differentiating into distinct cell types 3–6 Here we report differentiation marine Salpingoeca rosetta endows one its ability utilize insoluble ferric colloids improved growth expression cytochrome b561 iron reductase ( cytb561a ). This gene is an ortholog mammalian duodenal DCYTB ) reduces cations prior uptake gut epithelia 7 and part utilization toolkit choanoflagellates closest living relatives, animals, inherited from last common eukaryotic ancestor. In database oceanic metagenomes 8,9 , abundance transcripts positively correlates upwellings, which are major source environments 10 As predominant form 11,12 largely inaccessible cell-walled microbes 13,14 other phagotrophic eukaryotes may serve critical first acquiring phagocytosis then cycling nutrient pathways 13–15 These findings insight perform inform reconstructions early animal evolution where functionally became integrated whole at origin multicellularity 16–22

Язык: Английский

Cell differentiation controls iron assimilation in the choanoflagellate Salpingoeca rosetta

mSphere, Год журнала: 2025, Номер unknown

Опубликована: Фев. 26, 2025

ABSTRACT Marine microeukaryotes have evolved diverse cellular features that link their life histories to surrounding environments. How those dynamic intersect with the ecological functions of remains a frontier understanding roles in critical biogeochemical cycles. Choanoflagellates, phagotrophs cycle nutrients through filter feeding, provide models explore this intersection, for many choanoflagellate species transition between history stages by differentiating into distinct cell types. Here, we report differentiation marine Salpingoeca rosetta endows one its types ability utilize insoluble ferric colloids. These colloids are predominant form iron environments and largely inaccessible cell-walled microbes. Therefore, choanoflagellates other phagotrophic eukaryotes may serve cycling essential nutrient utilization pathways. We found S. can these via expression cytochrome b561 reductase ( cytb561a ). This gene mammalian ortholog, duodenal DCYTB ) reduces cations uptake gut epithelia, belong subgroup proteins biochemical contribute reduction activity. Overall, our findings insight perform inform reconstructions early animal evolution where functionally became an integrated whole at origin multicellularity. IMPORTANCE study examines how enables iron, nutrient. Choanoflagellates widespread, aquatic closest living relatives animals. Similar relatives, model choanoflagellate, , divides metabolic One type uses acquire colloids, key source ocean. also observed has three variants reductase, each properties likely lead differences they reduce iron. reductases variably distributed across ocean regions, suggesting role

Язык: Английский