Current Opinion in Cell Biology, Год журнала: 2023, Номер 85, С. 102261 - 102261
Опубликована: Окт. 28, 2023
Язык: Английский
BioEssays, Год журнала: 2024, Номер 46(12)
Опубликована: Авг. 28, 2024
Abstract Gastrulation is a key milestone in the development of an organism. It period cell proliferation and coordinated cellular rearrangement, that creates outline body plan. Our current understanding mammalian gastrulation has been improved by embryo culture, but there are still many open questions difficult to address because intrauterine embryos low number specimens. In case humans, additional difficulties associated with technical ethical challenges. Over last few years, pluripotent stem models being developed have potential become useful tools understand gastrulation. Here we review these special emphasis on gastruloids provide survey methods produce them robustly, their uses, relationship embryos, prospects as well limitations.
Язык: Английский
Процитировано
8
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Ноя. 22, 2023
ABSTRACT Gastruloids have recently emerged as an efficient four-dimensional model for studying some aspects of post-implantation embryonic patterning. They undergo gastrulation-like processes leading to the self-organization into highly reproducible biological objects. Here, we sought uncover molecular and cellular mechanism underlying this remarkable property. We report that competence is associated with a cell-specific coordination Cadherin switch. find N-Cadherin hinders gastruloids morphogenetic competence, its inactivation leads formation trunk-like structures in absence extra-cellular matrix analogues. In contrast, E-Cadherin repression by Snai1 critical self-organization: establishes repressive pace triggering pluripotency-associated transcription program chromatin landscape, thus allowing proper transition from E-to occur. Altogether, work integrates exit pluripotency cell differentiation, observed self-organizing potential gastruloids.
Язык: Английский
Процитировано
11
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Дек. 17, 2024
Mammalian embryonic size is tightly controlled with checkpoints and compensatory mechanisms correcting defects. Here, we take advantage of gastruloids, a stem cell embryoid system not subject to most controls, study the role in emergent properties mammalian embryogenesis. We report that gastruloids exhibit robust morphology transcriptional profiles within range. However, affects dynamics, and, outside range morphogenesis, precision anterior-posterior (AP) axial elongation. Gastruloid elongation exhibits active cellular contractility, requires planar polarity (PCP), adhesion cell-cell contact remodelling. Smaller initiate earlier, correlated an earlier Brachyury polarisation. expression increases tissue fluidity. Axis formation regulated by balance multifoci coalescence timing initiation programme. Sizes beyond can modify relative composition. Very small aggregates have increased neural fate bias, accompanied loss paraxial mesoderm mediated differences Nodal signalling activity.
Язык: Английский
Процитировано
3
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Июнь 22, 2023
Abstract Embryogenesis is brought about by the coordinated activities of different signaling pathways controlling cell fate specification and morphogenesis. In vertebrate gastrulation, both Nodal BMP play key roles in germ layer morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis still insufficiently understood. Here, we took a reductionist approach using zebrafish embryonic explants study coordination for We show that not only triggers explant elongation inducing mesendodermal progenitors but also suppressing activity at site mesendoderm induction. Ectopic blocks alignment oriented intercalations, processes drive elongation. Translating these ex vivo observations intact showed that, similar explants, renders dorsal domain less sensitive towards allow effective intercalations thus robust axis These findings suggest dual function maintaining low levels portion mesendoderm.
Язык: Английский
Процитировано
4
Development, Год журнала: 2024, Номер 151(4)
Опубликована: Фев. 15, 2024
ABSTRACT Embryogenesis results from the coordinated activities of different signaling pathways controlling cell fate specification and morphogenesis. In vertebrate gastrulation, both Nodal BMP play key roles in germ layer morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis is still insufficiently understood. Here, we took a reductionist approach using zebrafish embryonic explants study coordination for We show that triggers explant elongation by inducing mesendodermal progenitors but also suppressing activity at site mesendoderm induction. Consistent this, ectopic blocks alignment oriented intercalations, processes during elongation. Translating these ex vivo observations intact showed that, similar explants, suppresses effect on intercalations dorsal domain, thus allowing robust axis These findings suggest dual function effects portion mesendoderm.
Язык: Английский
Процитировано
1
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(23)
Опубликована: Май 31, 2024
A key feature of many developmental systems is their ability to self-organize spatial patterns functionally distinct cell fates. To ensure proper biological function, such must be established reproducibly, by controlling and even harnessing intrinsic extrinsic fluctuations. While the relevant molecular processes are increasingly well understood, we lack a principled framework quantify performance stochastic self-organizing systems. that end, introduce an information-theoretic measure for self-organized fate specification during embryonic development. We show proposed assesses total information content decomposes it into interpretable contributions corresponding positional correlational information. By optimizing measure, our provides normative theory circuits, which demonstrate on lateral inhibition, type proportioning, reaction-diffusion models self-organization. This paves way toward classification based common language, thereby organizing zoo implicated chemical mechanical signaling processes.
Язык: Английский
Процитировано
1
Emerging Topics in Life Sciences, Год журнала: 2023, Номер 7(4), С. 439 - 454
Опубликована: Дек. 14, 2023
Haematopoietic stem cells (HSCs) are the most extensively studied adult cells. Yet, six decades after their first description, reproducible and translatable generation of HSC in vitro remains an unmet challenge. production is confounded by multi-stage nature blood during development. Specification a late event embryonic depends on physical chemical cues which remain incompletely characterised. The precise molecular composition themselves understood, limiting approaches to track origin situ appropriate cellular, mechanical context. Embryonic material at point emergence limiting, highlighting need for model haematopoietic development current knowledge gaps can be addressed exploited enable production. Gastruloids pluripotent cell-derived 3-dimensional (3D) cellular aggregates recapitulate developmental events gastrulation early organogenesis with spatial temporal precision. self-organise multi-tissue structures upon minimal controlled external cues, amenable live imaging, screening, scaling physicochemical manipulation understand translate tissue formation. In this review, we consider potential gastruloids review strategies enhance progenitor We highlight possible achieve from gastruloids, discuss gastruloid systems illuminating specification.
Язык: Английский
Процитировано
3
Current Opinion in Cell Biology, Год журнала: 2023, Номер 85, С. 102261 - 102261
Опубликована: Окт. 28, 2023
Язык: Английский
Процитировано
1