Systematic screens for fertility genes essential for malaria parasite transmission reveal conserved aspects of sex in a divergent eukaryote
Cell Systems,
Год журнала:
2024,
Номер
15(11), С. 1075 - 1091.e6
Опубликована: Ноя. 1, 2024
Sexual
reproduction
in
malaria
parasites
is
essential
for
their
transmission
to
mosquitoes
and
offers
a
divergent
eukaryote
model
understand
the
evolution
of
sex.
Through
panel
genetic
screens
Plasmodium
berghei,
we
identify
348
sex
transmission-related
genes
define
roles
unstudied
as
putative
targets
transmission-blocking
interventions.
The
functional
data
provide
deeper
understanding
female
metabolic
reprogramming,
meiosis,
axoneme.
We
complex
SUN
domain
protein
(SUN1)
allantoicase
(ALLC1)
that
male
fertility
by
linking
microtubule
organizing
center
nuclear
envelope
enabling
mitotic
spindle
formation
during
gametogenesis.
Both
proteins
have
orthologs
mouse
testis,
raise
possibility
an
ancient
role
atypical
coupling
nucleus
Altogether,
our
unbiased
picture
molecular
processes
underpin
parasite
transmission.
A
record
this
paper's
transparent
peer
review
process
included
supplemental
information.
Язык: Английский
Functional consequences of reductive protein evolution in a minimal eukaryotic genome
Jason Jiang,
Rui Qu,
M Grigorescu
и другие.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Abstract
Microsporidia
are
parasites
with
the
smallest
known
eukaryotic
genomes.
The
extent
of
protein
loss
in
these
organisms
has
been
well
documented,
but
much
less
is
about
how
compaction
microsporidia
proteins
affects
their
function.
Taking
a
comparative
genomic
approach,
we
identified
orthologs
budding
yeast
and
show
that
enriched
for
essential
genes.
We
median
21%
shorter
than
its
counterpart
although
extensive
occurred
after
divergence
microsporidia,
reduced
sizes
were
already
present
microsporidian
relatives.
through
domain
lengths,
diminished
linker
loss,
having
lost
domains
yeast.
On
average,
34%
have
C-terminal
residues
function
yeast,
including
13
per
genome.
also
found
display
distinct
phylogenetic
patterns
losses
occurring
clade-specific
manner.
To
investigate
conservation
function,
used
complementation
assays
to
test
from
several
species
relative
Rozella
allomycis
.
These
experiments
reveal
most
cannot
complement
orthologs,
ability
three-fold
observed
R.
do
not
more
length
orthologs.
Altogether,
our
results
demonstrate
drastic
reduction
reductions
resulted
functional
fungal
ancestors.
Язык: Английский
Systematic screens for fertility genes essential for malaria parasite transmission reveal conserved aspects of sex in a divergent eukaryote
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 25, 2023
SUMMARY
Sexual
reproduction
in
malaria
parasites
is
essential
for
their
transmission
to
mosquitoes.
It
also
offers
a
divergent
eukaryote
model
understand
the
evolution
of
sex.
Through
panel
genetic
screens,
where
each
sex
Plasmodium
berghei
was
mutagenised
separately
with
barcoded
vectors,
we
identify
401
and
transmission-related
gene
functions
define
roles
hundreds
unstudied
fertility
genes
as
putative
targets
blocking
interventions.
The
functional
data
provide
deeper
understanding
female
metabolic
reprogramming,
meiosis
axoneme.
We
protein
complex
SUN
domain
protein,
SUN1,
moonlighting
allantoicase,
ALLC1,
that
male
by
linking
microtubule
organising
centre
nuclear
envelope
enabling
mitotic
spindle
formation
during
gametogenesis.
Both
proteins
have
orthologs
mouse
testis,
point
an
ancient
role
atypical
fertility.
Altogether,
our
unbiased
picture
molecular
processes
underpin
parasite
but
highlight
ancestral
aspects
evolved
close
last
eukaryotic
common
ancestor.
Язык: Английский