Unbiased quantification of the spatial distribution of murine cells using point pattern analysis DOI Creative Commons
Daniel Manrique‐Castano, Ayman ElAli

STAR Protocols, Год журнала: 2024, Номер 5(2), С. 102989 - 102989

Опубликована: Апрель 2, 2024

CNS injuries are associated with profound changes in cell organization. This protocol presents a stepwise approach to quantitatively describe the spatiotemporal glial rearrangement injured murine brain, which is applicable other biological contexts. Herein, we apply common immunolabeling of neurons and cells wide-field microscopy imaging. Then, employ computational tools for alignment Allen Brain Atlas, unbiased/automatic detection cells, generation point patterns, data analysis. For complete details on use execution this protocol, please refer Manrique-Castano et al.1

Язык: Английский

Unbiased quantification of the spatial distribution of murine cells using point pattern analysis DOI Creative Commons
Daniel Manrique‐Castano, Ayman ElAli

STAR Protocols, Год журнала: 2024, Номер 5(2), С. 102989 - 102989

Опубликована: Апрель 2, 2024

CNS injuries are associated with profound changes in cell organization. This protocol presents a stepwise approach to quantitatively describe the spatiotemporal glial rearrangement injured murine brain, which is applicable other biological contexts. Herein, we apply common immunolabeling of neurons and cells wide-field microscopy imaging. Then, employ computational tools for alignment Allen Brain Atlas, unbiased/automatic detection cells, generation point patterns, data analysis. For complete details on use execution this protocol, please refer Manrique-Castano et al.1

Язык: Английский

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