STAR Protocols, Journal Year: 2024, Volume and Issue: 5(2), P. 102989 - 102989
Published: April 2, 2024
CNS injuries are associated with profound changes in cell organization. This protocol presents a stepwise approach to quantitatively describe the spatiotemporal glial rearrangement injured murine brain, which is applicable other biological contexts. Herein, we apply common immunolabeling of neurons and cells wide-field microscopy imaging. Then, employ computational tools for alignment Allen Brain Atlas, unbiased/automatic detection cells, generation point patterns, data analysis. For complete details on use execution this protocol, please refer Manrique-Castano et al.1
Language: Английский