Histone variant H2BE controls activity-dependent gene expression and homeostatic scaling DOI Creative Commons
Emily R. Feierman, Alekh Paranjapye, Shaoyu Su

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 2, 2024

SUMMARY A cell’s ability to respond and adapt environmental stimuli relies in part on transcriptional programs controlled by histone proteins. Histones affect transcription through numerous mechanisms including replacement with variant forms that carry out specific functions. We recently identified the first widely expressed H2B variant, H2BE found it promotes is critical for neuronal function long-term memory. However, how regulated extracellular whether controls activity-dependent cellular plasticity remain unknown. used CUT&Tag RNA-sequencing of primary neurons, single-nucleus sequencing cortical tissue, multielectrode array recordings interrogate expression response role gene plasticity. find unlike Further, we show neurons lacking are unable mount proper responses both cultured animal models. Lastly, demonstrate knockout fail undergo electrophysiological changes associated homeostatic after stimulation. In summary, these data inversely correlated activity necessary responses, revealing instance a involved neurons.

Язык: Английский

Genetically- and spatially-defined basolateral amygdala neurons control food consumption and social interaction DOI Creative Commons
Hansol Lim, Yue Zhang, Christian Peters

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 11, 2024

Abstract The basolateral amygdala (BLA) contains discrete neuronal circuits that integrate positive or negative emotional information and drive the appropriate innate learned behaviors. Whether these consist of genetically-identifiable anatomically segregated neuron types, is poorly understood. Also, our understanding response patterns behavioral spectra BLA neurons limited. Here, we classified 11 glutamatergic cell clusters in mouse found several them were lateral versus basal amygdala, anterior posterior regions BLA. Two subpopulations innately responded to valence-specific, whereas one mixed - aversive social cues. Positive-valence promoted normal feeding, while selectivity fear learning interactions. These findings enhance type diversity spatial organization role distinct populations representing valence-specific stimuli.

Язык: Английский

Процитировано

6
Mimicking opioid analgesia in cortical pain circuits DOI Creative Commons
Justin G. James, Nora M. McCall,

Alex Hsu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 29, 2024

The anterior cingulate cortex plays a pivotal role in the cognitive and affective aspects of pain perception. Both endogenous exogenous opioid signaling within mitigate cortical nociception, reducing unpleasantness. However, specific functional molecular identities cells mediating analgesia remain elusive. Given complexity as sensory emotional experience, richness ethological pain-related behaviors, we developed standardized, deep-learning platform for deconstructing behavior dynamics associated with component mice-LUPE (Light aUtomated Pain Evaluator). LUPE removes human bias quantification accelerated analysis from weeks to hours, which leveraged discover that morphine altered attentional motivational behaviors akin humans. Through activity-dependent genetics single-nuclei RNA sequencing, identified ensembles nociceptive neuron-types expressing mu-opioid receptors. Tuning receptor expression these bidirectionally modulated analgesia. Moreover, employed synthetic promoter-driven approach cell-type optical chemical genetic viral therapies mimic morphine's pain-relieving effects cingulate, without reinforcement. This offers novel strategy precision management by targeting key circuit on-demand, non-addictive, effective

Язык: Английский

Процитировано

4
Endogenous opioid receptors and the feast or famine of maladaptive feeding DOI Creative Commons

Ames K. Sutton Hickey,

Bridget A. Matikainen‐Ankney

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 6, 2025

Maladaptive feeding comprises unhealthy eating patterns that jeopardize survival, including over- and underconsumption. These behaviors are often coordinated by endogenous opioid receptors (EORs). Here, we explore the involvement of EORs in obesity anorexia nervosa (AN), two disorders associated with dysregulated behavior relevant animal models. While seemingly opposing metabo-psychiatric states, our goal is to highlight common circuit synaptic mechanisms underlying AN a focus on EOR functionality. We examine neural substrates maladaptive comorbid conditions pain, suggesting role for EOR-driven plasticity pathogenesis both AN.

Язык: Английский

Процитировано

0
The efficacy of neurostimulation techniques for the management of chronic pain associated with bone disorders: A systematic review and meta-analysis DOI

Hassan A. Al-Ghanim,

Zainab Mohammed Aleid,

Saud Nayef Aldanyowi

и другие.

Surgical Neurology International, Год журнала: 2025, Номер 16, С. 137 - 137

Опубликована: Апрель 18, 2025

Background The management of chronic pain associated with bone problems has been accomplished by the use neurostimulation methods, such as spinal cord stimulation (SCS) and peripheral nerve (PNS). It is still unknown, however, how successful they are in comparison. effectiveness SCS PNS reducing enhancing functional results patients related to abnormalities was assessed this comprehensive review meta-analysis. Methods To find randomized controlled trials (RCTs) comparing or standard medical placebo/sham treatment adults disorders, a search PubMed, MEDLINE, Embase, CINAHL, Cochrane Central Register Controlled Trials, ClinicalTrials.gov carried out from start database until February 2024. main result absence discomfort. Opioid usage, status, quality life were secondary outcomes. technique used evaluate bias risk. risk ratios (RRs) standardized mean differences (SMDs) 95% confidence intervals (CIs) computed using random effects Results We included 20 RCTs total 2576 participants. In short-term (≤6 months) follow-up, both substantially higher alleviation than conventional care placebo/sham: SMD −0.87 (95% CI −1.19–−0.55), −0.56 −0.91–0.21). −0.71 −1.05–−0.37) −0.60 −1.03–−0.17) benefits maintained at long-term (>6 follow-up. physical emotional functioning, well life, also markedly enhanced PNS. shown that (RR 0.57, 0.44–0.74) 0.58, 0.43–0.77) reduced opioid usage. Conclusion When it comes improving functionality reduce linked problems, temporarily permanently. some individuals, may assist lowering consumption. Neurostimulation treatments be useful persistent diseases.

Язык: Английский

Процитировано

0
AxoDen: An Algorithm for the Automated Quantification of Axonal Density in defined Brain Regions DOI Creative Commons
R. Ortega, Emmy Li,

Oliver Joseph

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 31, 2024

Abstract The rodent brain contains 70,000,000+ neurons interconnected via complex axonal circuits with varying architectures. Neural pathologies are often associated anatomical changes in these projections and synaptic connections. Notably, density variations of local long-range increase or decrease as a function the strengthening weakening, respectively, information flow between regions. Traditionally, histological quantification inputs relied on assessing mean fluorescence intensity within rectangle placed region-of-inter-est. Despite yielding valuable insights, this conventional method is notably susceptible to background fluorescence, post-acquisition adjustments, inter-researcher variability. Additionally, it fails account for non-uniform innervation across regions, thus overlooking critical data such percentages distribution patterns. In response challenges, we introduce AxoDen, an open-source semi-automated platform designed speed rigor axon quantifications basic neuroscience discovery. AxoDen processes user-defined regions-of-interests incorporating dynamic thresholding grayscales-transformed images facilitate binarized pixel measure-ments. Thereby segregates image content into signal non-signal categories, effectively eliminating interference enabling exclusive measurement from projections. provides detailed accurate representations spatial distribution. AxoDen’s advanced yet user-friendly enhances reliability efficiency analysis facilitates access unbiased high-quality no technical coding experience required. freely available everyone tool dissecting patterns precisely defined

Язык: Английский

Процитировано

1
Histone variant H2BE controls activity-dependent gene expression and homeostatic scaling DOI Creative Commons
Emily R. Feierman, Alekh Paranjapye, Shaoyu Su

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 2, 2024

SUMMARY A cell’s ability to respond and adapt environmental stimuli relies in part on transcriptional programs controlled by histone proteins. Histones affect transcription through numerous mechanisms including replacement with variant forms that carry out specific functions. We recently identified the first widely expressed H2B variant, H2BE found it promotes is critical for neuronal function long-term memory. However, how regulated extracellular whether controls activity-dependent cellular plasticity remain unknown. used CUT&Tag RNA-sequencing of primary neurons, single-nucleus sequencing cortical tissue, multielectrode array recordings interrogate expression response role gene plasticity. find unlike Further, we show neurons lacking are unable mount proper responses both cultured animal models. Lastly, demonstrate knockout fail undergo electrophysiological changes associated homeostatic after stimulation. In summary, these data inversely correlated activity necessary responses, revealing instance a involved neurons.

Язык: Английский

Процитировано

0