A protein blueprint of the diatom CO2-fixing organelle
Cell,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Diatom pyrenoids are encased in a protein shell that enables efficient CO2 fixation
Cell,
Год журнала:
2024,
Номер
187(21), С. 5919 - 5934.e19
Опубликована: Окт. 1, 2024
Язык: Английский
In-cell architecture of the mitochondrial respiratory chain
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 4, 2024
Abstract
Mitochondria
produce
energy
through
oxidative
phosphorylation,
carried
out
by
five
membrane-bound
complexes
collectively
known
as
the
respiratory
chain.
These
work
in
concert
to
transfer
electrons
and
pump
protons,
leading
ATP
regeneration.
The
precise
organization
of
these
native
cells
is
debated,
notably
their
assembly
into
higher-order
supercomplexes
called
respirasomes.
Here,
we
use
situ
cryo-electron
tomography
visualize
structures
several
major
mitochondrial
inside
Chlamydomonas
reinhardtii
cells.
synthases
are
segregated
curved
flat
crista
membrane
domains,
respectively.
Respiratory
I,
III,
IV
assemble
a
single
type
respirasome,
from
which
determined
5
Å-resolution
structure
showing
binding
electron
carrier
cytochrome
c
.
Combined
with
single-particle
microscopy
reconstruction
at
2.4
Å
resolution,
detailed
model
how
interact
each
other
mitochondria.
Язык: Английский
A Protein Blueprint of the Diatom CO2-Fixing Organelle
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 26, 2023
Abstract
Diatoms
are
central
to
the
global
carbon
cycle.
At
heart
of
diatom
fixation
is
an
overlooked
organelle
called
pyrenoid,
where
concentrated
CO
2
delivered
densely
packed
Rubisco.
Diatom
pyrenoids
fix
approximately
one-fifth
,
but
virtually
nothing
known
about
this
in
diatoms.
Using
large-scale
fluorescence
protein
tagging
and
affinity
purification-mass
spectrometry,
we
generate
a
high-confidence
spatially-defined
protein-protein
interaction
network
for
pyrenoid.
Within
our
pyrenoid
10
proteins
with
no
function.
We
show
that
six
these
form
static
shell
encapsulating
Rubisco
matrix
critical
structural
integrity,
shape,
Although
not
conserved
at
sequence
level,
shares
some
architectural
similarities
prokaryotic
carboxysomes.
Collectively,
results
support
convergent
evolution
across
two
main
plastid
lineages
uncover
major
functional
component
fixation.
Язык: Английский
Molecular architecture of thylakoid membranes within intact spinach chloroplasts
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 26, 2024
Abstract
Thylakoid
membranes
coordinate
the
light
reactions
of
photosynthesis
across
multiple
scales,
coupling
architecture
an
elaborate
membrane
network
to
spatial
organization
individual
protein
complexes
embedded
within
this
network.
Previously,
we
used
in
situ
cryo-
electron
tomography
(cryo-ET)
reveal
native
thylakoid
green
alga
Chlamydomonas
reinhardtii
[1]
and
then
map
molecular
these
thylakoids
with
single-molecule
precision
[2].
However,
it
remains
be
shown
how
generalizable
algal
blueprint
is
vascular
plants,
which
possess
distinct
subdivided
into
grana
stacks
interconnected
by
non-stacked
stromal
lamellae.
Here,
continue
our
cryo-ET
investigation
intact
chloroplasts
isolated
from
spinach
(
Spinacia
oleracea
).
We
visualize
fine
ultrastructural
details
membranes,
as
well
interactions
between
plastoglobules.
apply
further
develop
AI-based
computational
approaches
for
automated
segmentation
picking
[3],
enabling
us
quantify
photosynthetic
plane
adjacent
stacked
membranes.
Our
analysis
reveals
that,
despite
different
3D
architecture,
plants
algae
strikingly
similar.
In
contrast
plant
thylakoids,
where
semi-
crystalline
arrays
photosystem
II
(PSII)
appear
hold
some
together,
find
that
PSII
non-crystalline
has
uniform
concentration
both
Similar
C.
,
observe
strict
lateral
heterogeneity
PSI
at
boundary
appressed
non-appressed
domains,
no
evidence
a
margin
region
have
been
proposed
intermix.
Based
on
measurements,
support
simple
two-domain
model
plants.
Язык: Английский
The big chill: Growth of in situ structural biology with cryo-electron tomography
QRB Discovery,
Год журнала:
2024,
Номер
5
Опубликована: Янв. 1, 2024
Abstract
In
situ
structural
biology
with
cryo-electron
tomography
(cryo-ET)
and
subtomogram
averaging
(StA)
is
evolving
as
a
major
method
to
understand
the
structure,
function,
interactions
of
biological
molecules
in
cells
single
experiment.
Since
its
inception,
has
matured
some
stellar
highlights
further
opportunities
broaden
applications.
this
short
review,
I
want
provide
personal
perspective
on
developments
cryo-ET
have
seen
it
for
last
~20
years
outline
steps
that
led
success.
This
my
eyes
junior
researcher
view
present
past
hardware
software
cryo-ET.
Язык: Английский