Wastewater testing during the South African 2022-2023 measles outbreak demonstrates the potential of environmental surveillance to support measles elimination DOI Creative Commons
Nkosenhle Ndlovu, Victor Mabasa,

Chenoa Sankar

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 2, 2024

Abstract Background Sensitive clinical surveillance and high vaccination coverage are required to meet the WHO 2030 measles elimination target. Whilst wastewater environmental (WES) has proven usefulness in polio surveillance, it not been applied control. We describe development of digital RT-PCR (RT-dPCR) for detection quantification virus (MeV) wastewater, application retained concentrated samples obtained from 28 national sentinel SARS-CoV-2 sites 19 localised collection points a single province before, during after outbreak over 2,000 laboratory-confirmed cases. Methods validated RT-dPCR assay incorporating WHO-recommended primers differentiation wild-type vaccine genotypes. this by ultrafiltration stored at −20°C. compared findings district epidemiological weeks with case data fever-rash districts adequate indicators (>2/100,000 non-measles cases) Findings Amongst 2,149 concentrates between 16 February 2021 08 March 2024, we identified 43 (2%) containing MeV RNA concentrations ranging 2,04-6,11 genome copies/mL. genotype A (vaccine strain) was co-detected 6 instances along non-A (wild-type) Comparison week 27 district-time where least one positive sample identified, which 14 (52%) reported that same week. samples, detected 13/27 (48%) time-district pairs when failed detect (N=127), collected (11%) Interpretation Wastewater may be useful complementary tool identification ongoing circulation. rates improved real-time testing limit degradation concentration nucleic acid extraction processes. Ongoing should conducted order understand role WES control elimination. Funding This work funded BMGF (INV-049271)

Язык: Английский

Detection of Measles Virus Genotype A in a Non-Endemic Wastewater Setting: Insights from Measles Wastewater and Environmental Monitoring in Canada’s Capital Region DOI Creative Commons

Emma Tomalty,

Élisabeth Mercier, Lakshmi Pisharody

и другие.

Environmental Science & Technology Letters, Год журнала: 2025, Номер 12(2), С. 124 - 129

Опубликована: Фев. 2, 2025

The recent global resurgence of measles in 2023–2024, despite vaccine preventability, underscores a critical public health issue, largely due to reduced vaccination coverage during the SARS-CoV-2 pandemic. In response, Ottawa Public Health intensified efforts 2023 and 2024. Additionally, research initiative began April 2024 monitor wastewater for virus (MeV) using established environmental surveillance (WES) protocols. Unexpected positive MeV detections through RT-qPCR wastewater─despite no active regional cases─prompted genotypic retrospective analyses archived RNA samples dating back 2020. analysis identified detection belong genotype A, progenitor strain viral vaccines, marking first report large catchment area. Linear regression revealed aligned with by Health. These findings emphasize importance integrating into WES practices mitigate possible confounding factors, such as shedding wastewater. this highlights potential applications complementary tool. Implementing study WES, other re-emerging viruses, could improve response resource allocation.

Язык: Английский

Процитировано

0
Wastewater testing during the South African 2022-2023 measles outbreak demonstrates the potential of environmental surveillance to support measles elimination DOI Creative Commons
Nkosenhle Ndlovu, Victor Mabasa,

Chenoa Sankar

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 2, 2024

Abstract Background Sensitive clinical surveillance and high vaccination coverage are required to meet the WHO 2030 measles elimination target. Whilst wastewater environmental (WES) has proven usefulness in polio surveillance, it not been applied control. We describe development of digital RT-PCR (RT-dPCR) for detection quantification virus (MeV) wastewater, application retained concentrated samples obtained from 28 national sentinel SARS-CoV-2 sites 19 localised collection points a single province before, during after outbreak over 2,000 laboratory-confirmed cases. Methods validated RT-dPCR assay incorporating WHO-recommended primers differentiation wild-type vaccine genotypes. this by ultrafiltration stored at −20°C. compared findings district epidemiological weeks with case data fever-rash districts adequate indicators (>2/100,000 non-measles cases) Findings Amongst 2,149 concentrates between 16 February 2021 08 March 2024, we identified 43 (2%) containing MeV RNA concentrations ranging 2,04-6,11 genome copies/mL. genotype A (vaccine strain) was co-detected 6 instances along non-A (wild-type) Comparison week 27 district-time where least one positive sample identified, which 14 (52%) reported that same week. samples, detected 13/27 (48%) time-district pairs when failed detect (N=127), collected (11%) Interpretation Wastewater may be useful complementary tool identification ongoing circulation. rates improved real-time testing limit degradation concentration nucleic acid extraction processes. Ongoing should conducted order understand role WES control elimination. Funding This work funded BMGF (INV-049271)

Язык: Английский

Процитировано

2