Biophysical aspect of assembly and regulation of nuclear bodies scaffolded by architectural RNA

Journal of Molecular Biology, Год журнала: 2025, Номер 437(9), С. 169016 - 169016

Опубликована: Фев. 18, 2025

Язык: Английский

RNA gradients can guide condensates toward promoters: implications for enhancer-promoter contacts and condensate-promoter kissing

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 6, 2024

We study how protein condensates respond to a site of active RNA transcription (i.e., gene promoter) due electrostatic protein-RNA interactions. Our results indicate that can show directed motion towards the promoter, driven by gradients in concentration. Analytical theory, consistent with simulations, predicts droplet velocity has non-monotonic dependence on distance promoter. explore consequences this gradient-sensing mechanism for enhancer-promoter (E-P) communication using polymer simulations intervening chromatin chain. Directed enhancer-bound can, together loop extrusion cohesin, collaboratively increase contact probability. Finally, we investigate under which conditions exhibit oscillations their morphology and Oscillatory dynamics are caused delayed response condensate-promoter negative feedback from accumulation at charge repulsion.

Язык: Английский

Biophysical Aspect of Assembly and Regulation of Nuclear Bodies Scaffolded by Architectural Rna

Опубликована: Янв. 1, 2024

Язык: Английский