SN Applied Sciences,
Год журнала:
2020,
Номер
2(4)
Опубликована: Март 10, 2020
Abstract
Bottom-up
synthetic
biology
aims
to
construct
mimics
of
cellular
structure
and
behaviour
known
as
artificial
cells
from
a
small
number
molecular
components.
The
development
this
nascent
field
has
coupled
new
insights
in
with
large
translational
potential
for
application
fields
such
drug
delivery
biosensing.
Multiple
approaches
have
been
applied
create
cell
mimics,
many
efforts
focusing
on
phospholipid-based
systems.
This
mini-review
focuses
different
incorporating
motifs
tools
lipid
membrane
functionalization
construction.
Such
range
chemical
functional
groups
components
extant
that
can
be
arranged
‘plug-and-play’
approach
which
is
hard
replicate
living
Rationally
designed
possess
the
promise
complex
biomimetic
minimal,
highly
engineered
networks.
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2016,
Номер
8(10), С. a006072 - a006072
Опубликована: Окт. 1, 2016
Stress-signaling
pathways
are
evolutionarily
conserved
and
play
an
important
role
in
the
maintenance
of
homeostasis.
These
also
critical
for
adaptation
to
new
cellular
environments.
The
endoplasmic
reticulum
(ER)
unfolded
protein
response
(UPR)
is
activated
by
biosynthetic
stress
leads
a
compensatory
increase
ER
function.
JNK
p38
MAPK
signaling
control
adaptive
responses
intracellular
extracellular
stresses,
including
environmental
changes
such
as
UV
light,
heat,
hyperosmotic
conditions,
exposure
inflammatory
cytokines.
Metabolic
caused
high-fat
diet
represents
example
stimulus
that
coordinately
activates
both
UPR
JNK/p38
pathways.
Chronic
activation
these
stress-response
ultimately
causes
metabolic
associated
with
obesity
altered
insulin
sensitivity.
pathways,
therefore,
represent
potential
targets
therapeutic
intervention
other
disease
processes.
Essays in Biochemistry,
Год журнала:
2018,
Номер
62(4), С. 487 - 500
Опубликована: Окт. 4, 2018
Systems
biology
is
an
integrative
discipline
connecting
the
molecular
components
within
a
single
biological
scale
and
also
among
different
scales
(e.g.
cells,
tissues
organ
systems)
to
physiological
functions
organismal
phenotypes
through
quantitative
reasoning,
computational
models
high-throughput
experimental
technologies.
uses
wide
range
of
methodologies
decode
information
flow
from
genes,
proteins
other
subcellular
signaling,
regulatory
functional
pathways
control
cell,
tissue,
level
functions.
The
methods
used
in
systems
provide
systems-level
insights
understand
interactions
dynamics
at
various
scales,
tissues,
organs
organisms.
In
recent
years,
framework
has
enabled
research
pharmacology
precision
medicine
for
complex
diseases.
Here,
we
present
brief
overview
current
biology.
Biochemical Journal,
Год журнала:
2023,
Номер
480(1), С. 1 - 23
Опубликована: Янв. 6, 2023
RAS
proteins
regulate
most
aspects
of
cellular
physiology.
They
are
mutated
in
30%
human
cancers
and
4%
developmental
disorders
termed
Rasopathies.
cycle
between
active
GTP-bound
inactive
GDP-bound
states.
When
active,
they
can
interact
with
a
wide
range
effectors
that
control
fundamental
biochemical
biological
processes.
Emerging
evidence
suggests
not
simple
on/off
switches
but
sophisticated
information
processing
devices
compute
cell
fate
decisions
by
integrating
external
internal
cues.
A
critical
component
this
function
is
the
dynamic
regulation
activation
downstream
signaling
allows
to
produce
rich
nuanced
spectrum
outputs.
We
discuss
recent
findings
how
dynamics
its
regulated.
Starting
from
structural
properties
wild-type
mutant
their
cycle,
we
examine
higher
molecular
assemblies,
effector
interactions
outputs,
all
under
aspect
regulation.
also
consider
computational
mathematical
modeling
approaches
contribute
analyze
understand
pleiotropic
functions
health
disease.
Annual Review of Microbiology,
Год журнала:
2019,
Номер
73(1), С. 507 - 528
Опубликована: Июнь 21, 2019
Perceiving
environmental
and
internal
information
reacting
in
adaptive
ways
are
essential
attributes
of
living
organisms.
Two-component
systems
relevant
protein
machineries
from
prokaryotes
lower
eukaryotes
that
enable
cells
to
sense
process
signals.
Implicating
sensory
histidine
kinases
response
regulator
proteins,
both
components
take
advantage
phosphorylation
flexibility
switch
conformations
a
signal-dependent
way.
Dozens
two-component
act
simultaneously
any
given
cell,
challenging
our
understanding
about
the
means
ensure
proper
connectivity.
This
review
dives
into
molecular
level,
attempting
summarize
an
emerging
picture
how
cognate
regulators
achieve
required
efficiency,
specificity,
directionality
signaling
pathways,
properties
rely
on
protein:protein
interactions.
α
helices
carry
through
long
distances,
fine
combination
loose
specific
kinase/regulator
interactions,
malleable
reaction
centers
built
when
two
meet
emerge
as
universal
principles.
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2015,
Номер
7(4), С. a005934 - a005934
Опубликована: Апрель 1, 2015
Evren
U.
Azeloglu
and
Ravi
Iyengar
Department
of
Pharmacology
Systems
Therapeutics
Biology
Center
New
York,
Mount
Sinai
School
Medicine,
York
10029
Correspondence:
ravi.iyengar{at}mssm.edu
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2016,
Номер
8(6), С. a005918 - a005918
Опубликована: Июнь 1, 2016
Michael
J.
Lee
and
B.
Yaffe
David
H.
Koch
Institute
for
Integrative
Cancer
Research
at
MIT,
Department
of
Biology
Biological
Engineering,
Massachusetts
Technology,
Cambridge,
02139
Correspondence:
myaffe{at}mit.edu
Cell Reports,
Год журнала:
2019,
Номер
28(13), С. 3486 - 3496.e6
Опубликована: Сен. 1, 2019
The
target
of
rapamycin
complex
1
(TORC1)
is
a
master
regulator
cell
homeostasis,
which
promotes
anabolic
reactions
and
synchronously
inhibits
catabolic
processes
such
as
autophagy-mediated
protein
degradation.
Its
prime
autophagy
Atg13,
subunit
the
Atg1
kinase
that
acts
gatekeeper
canonical
autophagy.
To
study
whether
activities
TORC1
are
coupled
through
additional,
more
intricate
control
mechanisms
than
simply
this
linear
pathway,
we
analyzed
epistatic
relationship
between
by
using
quantitative
phosphoproteomics.
Our
in
vivo
data,
combined
with
targeted
vitro
assays,
not
only
uncover
numerous
effectors,
but
also
suggest
distinct
bi-directional
regulatory
feedback
loops
characterize
Atg29
commonly
regulated
downstream
both
Atg1.
Thus,
an
exquisitely
multilayered
network
appears
to
coordinate
robustly
tune
response
nutritional
cues.
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(21), С. 11868 - 11868
Опубликована: Ноя. 1, 2021
How
can
single
cells
without
nervous
systems
perform
complex
behaviours
such
as
habituation,
associative
learning
and
decision
making,
which
are
considered
the
hallmark
of
animals
with
a
brain?
Are
there
molecular
that
underlie
cognitive
properties
equivalent
to
those
This
review
follows
development
idea
brains
from
Darwin’s
“root
brain
hypothesis”,
through
bacterial
chemotaxis,
recent
discovery
neuron-like
r-protein
networks
in
ribosome.
By
combining
structural
biology
view
Bayesian
approach,
this
explores
evolutionary
labyrinth
information
processing
across
scales.
Ribosomal
protein
open
window
into
what
were
probably
earliest
signalling
emerge
before
radiation
three
kingdoms.
While
ribosomal
characterised
by
long-lasting
interactions
between
their
nodes,
cell
essentially
based
on
transient
interactions.
As
corollary,
while
signals
propagated
persistent
may
be
ephemeral,
whose
constrain
diffusing
cytoplasm
durable
time,
post-translational
modifications
proteins
or
second
messenger
synthesis.
The
duration
nature
signals,
turn,
implies
different
mechanisms
for
integration
multiple
making.
Evolution
then
reinvented
metazoans.
simple
display
architectural
functional
analogies
comparison
could
suggest
scale
invariance
processing.
At
level,
significant
complexification
eukaryotic
is
associated
burst
acquisition
new
conserved
aromatic
amino
acids.
Knowing
residues
play
critical
role
allosteric
receptors
channels,
observation
suggests
general
π
charged
acids
signal
We
think
these
findings
provide
basis
designing
future
computers
organic
processors.
PLoS Biology,
Год журнала:
2023,
Номер
21(5), С. e3001665 - e3001665
Опубликована: Май 30, 2023
Epithelial
repair
relies
on
the
activation
of
stress
signaling
pathways
to
coordinate
tissue
repair.
Their
deregulation
is
implicated
in
chronic
wound
and
cancer
pathologies.
Using
TNF-α/Eiger-mediated
inflammatory
damage
Drosophila
imaginal
discs,
we
investigate
how
spatial
patterns
behaviors
arise.
We
find
that
Eiger
expression,
which
drives
JNK/AP-1
signaling,
transiently
arrests
proliferation
cells
center
associated
with
a
senescence
program.
This
includes
production
mitogenic
ligands
Upd
family,
allows
JNK/AP-1-signaling
act
as
paracrine
organizers
regeneration.
Surprisingly,
cell-autonomously
suppress
via
Ptp61F
Socs36E,
both
negative
regulators
JAK/STAT
signaling.
As
suppressed
at
damage,
compensatory
occurs
by
periphery.
Mathematical
modelling
suggests
cell-autonomous
mutual
repression
between
core
regulatory
network
essential
spatially
separate
into
bistable
domains
distinct
cellular
tasks.
Such
stratification
for
proper
repair,
coactivation
same
creates
conflicting
signals
cell
cycle
progression,
leading
excess
apoptosis
senescently
stalled
organize
field.
Finally,
demonstrate
separation
senescent
proliferative
not
only
upon
but
also
Ras
V12
,
scrib
tumors.
Revealing
this
previously
uncharacterized
JNK/AP-1,
JAK/STAT,
has
important
implications
our
conceptual
understanding
pathologies,
tumor
microenvironments.