Proceedings of the National Academy of Sciences,
Год журнала:
2022,
Номер
119(32)
Опубликована: Авг. 5, 2022
Animal
tissues
comprise
diverse
cell
types.
However,
the
mechanisms
controlling
number
of
each
type
within
tissue
compartments
remain
poorly
understood.
Here,
we
report
that
different
types
utilize
distinct
strategies
to
control
population
numbers.
Proliferation
fibroblasts,
stromal
cells
important
for
integrity,
is
limited
by
space
availability.
In
contrast,
proliferation
macrophages,
innate
immune
involved
in
defense,
repair,
and
homeostasis,
constrained
growth
factor
Examination
density-dependent
gene
expression
fibroblasts
revealed
Hippo
TGF-β
target
genes
are
both
regulated
density.
We
found
YAP1,
transcriptional
coactivator
signaling
pathway,
directly
regulates
Csf1
,
lineage-specific
through
an
enhancer
specifically
active
fibroblasts.
Activation
YAP1
elevates
sufficient
increase
macrophages
at
steady
state.
Our
data
also
suggest
programs
change
with
density
may
result
from
sensing
mechanical
force
actin-dependent
mechanisms.
Altogether,
demonstrate
two
modes
connected
coordinated
regulate
numbers
Sensing
environment
serve
as
a
general
strategy
composition.
Cold Spring Harbor Perspectives in Medicine,
Год журнала:
2015,
Номер
5(4), С. a006098 - a006098
Опубликована: Апрель 1, 2015
Richard
Sever1
and
Joan
S.
Brugge2
1Cold
Spring
Harbor
Laboratory,
Cold
Harbor,
New
York
11724
2Harvard
Medical
School,
Department
of
Cell
Biology,
Boston,
Massachusetts
02115
Correspondence:
joan_brugge{at}hms.harvard.edu
Frontiers in Genetics,
Год журнала:
2019,
Номер
10
Опубликована: Фев. 28, 2019
Cancer
is
a
multistep
disease
driven
by
the
activation
of
specific
oncogenic
pathways
concomitantly
with
loss
function
tumor
suppressor
genes
that
act
as
sentinels
to
control
physiological
growth.
The
conservation
most
these
signaling
in
Drosophila,
and
ability
easily
manipulate
them
genetically,
has
made
fruit
fly
useful
model
organism
study
cancer
biology.
In
this
review
we
outline
basic
mechanisms
conserved
between
humans
flies
responsible
inducing
uncontrolled
growth
development.
Second,
describe
classic
novel
Drosophila
models
used
different
cancers,
objective
discuss
their
strengths
limitations
on
use
identify
signals
driving
cell
autonomously
within
organs,
drug
discovery
for
therapeutic
approaches.
Frontiers in Pharmacology,
Год журнала:
2019,
Номер
10
Опубликована: Июль 26, 2019
Cancer,
a
complex
yet
common
disease,
is
caused
by
uncontrolled
cell
division
and
abnormal
growth
due
to
variety
of
gene
mutations.
Seeking
effective
treatments
for
cancer
major
research
focus
as
the
incidence
on
rise
drug
resistance
existing
anti-cancer
drugs
concern.
Natural
products
have
potential
yield
unique
molecules
combinations
substances
that
may
be
against
with
relatively
low
toxicity/better
side
effect
profile
compared
standard
anticancer
therapy.
Drug
discovery
work
natural
has
demonstrated
compounds
display
wide
range
biological
activities
correlating
effects.
In
this
review,
we
discuss
formononetin
(C16H12O4),
which
originates
mainly
from
red
clovers
Chinese
herb
Astragalus
membranaceus.
The
compound
comes
class
7-hydroisoflavones
substitution
methoxy
group
at
position
4.
Formononetin
elicits
antitumourigenic
properties
in
vitro
vivo
modulating
numerous
signaling
pathways
induce
apoptosis
(by
intrinsic
pathway
involving
Bax,
Bcl-2
caspase-3
proteins),
cycle
arrest
regulating
mediators
like
cyclin
A,
B1
D1),
suppress
proliferation
STAT
activation,
PI3K/AKT
MAPK
pathway)
inhibit
invasion
factors
VEGF
FGF2,
MMP-2
MMP-9
proteins).
Co-treatment
other
chemotherapy
such
bortezomib,
LY2940002,
U0126,
sunitinib,
epirubicin,
doxorubicin,
temozolomide
metformin
enhance
both
respective
through
synergistic
effect.
Compiling
evidence
thus
far
highlights
promising
candidate
chemoprevention
chemotherapy.
International Journal of Environmental Research and Public Health,
Год журнала:
2020,
Номер
17(18), С. 6811 - 6811
Опубликована: Сен. 18, 2020
The
production
of
plastic
products,
which
requires
phthalate
plasticizers,
has
resulted
in
the
problems
for
human
health,
especially
that
reproductive
health.
Phthalate
exposure
can
induce
disorders
at
various
regulatory
levels.
aim
this
review
was
to
compile
evidence
concerning
association
between
phthalates
and
diseases,
phthalates-induced
disorders,
their
possible
endocrine
intracellular
mechanisms.
Phthalates
may
alterations
puberty,
development
testicular
dysgenesis
syndrome,
cancer,
fertility
both
males
females.
At
hormonal
level,
modify
release
hypothalamic,
pituitary,
peripheral
hormones.
interfere
with
nuclear
receptors,
membrane
signaling
pathways,
modulate
gene
expression
associated
reproduction.
To
understand
treat
adverse
effects
on
it
is
essential
expand
current
knowledge
mechanism
action
organism.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(5), С. 4822 - 4822
Опубликована: Март 2, 2023
The
trace
element
zinc
(Zn)
displays
a
wide
range
of
biological
functions.
Zn
ions
control
intercellular
communication
and
intracellular
events
that
maintain
normal
physiological
processes.
These
effects
are
achieved
through
the
modulation
several
Zn-dependent
proteins,
including
transcription
factors
enzymes
key
cell
signaling
pathways,
namely
those
involved
in
proliferation,
apoptosis,
antioxidant
defenses.
Efficient
homeostatic
systems
carefully
regulate
concentrations.
However,
perturbed
homeostasis
has
been
implicated
pathogenesis
chronic
human
diseases,
such
as
cancer,
diabetes,
depression,
Wilson’s
disease,
Alzheimer’s
other
age-related
diseases.
This
review
focuses
on
Zn’s
roles
survival/death,
DNA
repair
mechanisms,
outlines
some
targets,
addresses
therapeutic
potential
supplementation
Epithelial
mesenchymal
transition
(EMT)
and
epithelial
(MET)
are
genetic
determinants
of
cellular
plasticity.
These
programs
operate
in
physiological
(embryonic
development,
wound
healing)
pathological
(organ
fibrosis,
cancer)
conditions.
In
cancer,
EMT
MET
interfere
with
various
signalling
pathways
at
different
levels.
This
results
gross
alterations
the
gene
expression
programs,
which
affect
most,
if
not
all
hallmarks
such
as
response
to
proliferative
death-inducing
signals,
tumorigenicity,
cell
stemness.
cancer
cells
involves
large
scale
reorganisation
cytoskeleton,
loss
integrity,
gain
traits,
type
migration.
this
regard,
EMT/MET
plasticity
is
highly
relevant
Go-or-Grow
concept,
postulates
dichotomous
relationship
between
motility
proliferation.
The
decisions
critically
important
processes
takes
central
stage,
mobilisation
stem
during
healing,
relapse,
metastasis.
Here
we
outline
maintenance
quiescence
metastatic
niches,
focusing
on
implication
regulatory
networks
switches.
particular,
discuss
analogy
residing
hybrid
quasi-mesenchymal
states
GAlert,
an
intermediate
phase
allowing
quiescent
enter
cycle
rapidly.
Nature Biomedical Engineering,
Год журнала:
2023,
Номер
7(7), С. 943 - 958
Опубликована: Апрель 3, 2023
Methods
for
the
analysis
of
cell
secretions
at
single-cell
level
only
provide
semiquantitative
endpoint
readouts.
Here
we
describe
a
microwell
array
real-time
spatiotemporal
monitoring
extracellular
from
hundreds
single
cells
in
parallel.
The
incorporates
gold
substrate
with
arrays
nanometric
holes
functionalized
receptors
specific
analyte,
and
is
illuminated
light
spectrally
overlapping
device's
spectrum
extraordinary
optical
transmission.
Spectral
shifts
surface
plasmon
resonance
resulting
analyte-receptor
bindings
around
secreting
are
recorded
by
camera
as
variations
intensity
transmitted
while
machine-learning-assisted
tracking
eliminates
influence
movements.
We
used
to
characterize
antibody-secretion
profiles
hybridoma
rare
subset
antibody-secreting
sorted
human
donor
peripheral
blood
mononuclear
cells.
High-throughput
measurements
secretory
will
aid
study
physiological
mechanisms
governing
protein
secretion.
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2012,
Номер
4(10), С. a005942 - a005942
Опубликована: Окт. 1, 2012
Nicholas
Rhind1
and
Paul
Russell2
1Department
of
Biochemistry
Molecular
Pharmacology,
University
Massachusetts
Medical
School,
Worcester,
01605
2Department
Biology,
Department
Cell
The
Scripps
Research
Institute,
La
Jolla,
California
92037
Correspondence:
nick.rhind{at}umassmed.edu
Biogerontology,
Год журнала:
2018,
Номер
19(6), С. 547 - 566
Опубликована: Фев. 28, 2018
Ageing
leads
to
dramatic
changes
in
the
physiology
of
many
different
tissues
resulting
a
spectrum
pathology.
Nonetheless,
lines
evidence
suggest
that
ageing
is
driven
by
highly
conserved
cell
intrinsic
processes,
and
set
unifying
hallmarks
has
been
defined.
Here,
we
survey
reports
age-linked
basal
gene
expression
across
eukaryotes
from
yeast
human
identify
six
cellular
ageing:
downregulation
genes
encoding
mitochondrial
proteins;
protein
synthesis
machinery;
dysregulation
immune
system
genes;
reduced
growth
factor
signalling;
constitutive
responses
stress
DNA
damage;
mRNA
processing.
These
encompass
widely
reported
features
such
as
increased
senescence
inflammation,
electron
transport
chain
activity
ribosome
synthesis,
but
also
reveal
surprising
lack
known
stresses.
We
discuss
how
existence
transcriptomic
relates
genome-wide
epigenetic
differences
underlying
clocks,
changing
transcriptome
results
proteomic
alterations
where
data
available
variations
characteristic
ageing.
Identification
events
occur
during
distant
organisms
should
be
informative
mechanisms
ageing,
provide
additional
biomarkers
assess
effects
diet
other
environmental
factors
on
rate
