Auxin signaling: Research advances over the past 30 years

Journal of Integrative Plant Biology, Год журнала: 2022, Номер 64(2), С. 371 - 392

Опубликована: Янв. 12, 2022

Auxin, one of the first identified and most widely studied phytohormones, has been will remain a hot topic in plant biology. After more than century passionate exploration, mysteries its synthesis, transport, signaling, metabolism have largely unlocked. Due to rapid development new technologies, methods, genetic materials, study auxin entered fast lane over past 30 years. Here, we highlight advances understanding including perception, responses, TRANSPORT INHIBITOR RESPONSE 1 AUXIN SIGNALING F-boxes (TIR1/AFBs)-mediated transcriptional non-transcriptional branches, epigenetic regulation signaling. We also focus on feedback inhibition mechanisms that prevent over-amplification signals. In addition, cover TRANSMEMBRANE KINASE-mediated non-canonical which converges with TIR1/AFBs-mediated coordinate growth development. The identification additional signaling components their continue open avenues research this field, leading an increasingly deeper, comprehensive how signals are interpreted at cellular level regulate

Язык: Английский

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ABP1–TMK auxin perception for global phosphorylation and auxin canalization

Nature, Год журнала: 2022, Номер 609(7927), С. 575 - 581

Опубликована: Сен. 7, 2022

Язык: Английский

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Adenylate cyclase activity of TIR1/AFB auxin receptors in plants

Nature, Год журнала: 2022, Номер 611(7934), С. 133 - 138

Опубликована: Окт. 26, 2022

Язык: Английский

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Structural insights into auxin recognition and efflux by Arabidopsis PIN1

Nature, Год журнала: 2022, Номер 609(7927), С. 611 - 615

Опубликована: Авг. 2, 2022

Polar auxin transport is unique to plants and coordinates their growth development1,2. The PIN-FORMED (PIN) transporters exhibit highly asymmetrical localizations at the plasma membrane drive polar transport3,4; however, structures mechanisms remain largely unknown. Here, we report three inward-facing conformation of Arabidopsis thaliana PIN1: apo state, bound natural indole-3-acetic acid (IAA), in complex with inhibitor N-1-naphthylphthalamic (NPA). transmembrane domain PIN1 shares a conserved NhaA fold5. In substrate-bound structure, IAA coordinated by both hydrophobic stacking hydrogen bonding. NPA competes for same site intracellular pocket, but much higher affinity. These findings inform our understanding substrate recognition PINs set up framework future research on directional transport, one most crucial processes underlying plant development.

Язык: Английский

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Plant Hormone Transport and Localization: Signaling Molecules on the Move

Annual Review of Plant Biology, Год журнала: 2023, Номер 74(1), С. 453 - 479

Опубликована: Март 8, 2023

Plant hormones are a group of small signaling molecules produced by plants at very low concentrations that have the ability to move and function distal sites. Hormone homeostasis is critical balance plant growth development regulated multiple levels, including hormone biosynthesis, catabolism, perception, transduction. In addition, over short long distances regulate various developmental processes responses environmental factors. Transporters coordinate these movements, resulting in maxima, gradients, cellular subcellular sinks. Here, we summarize current knowledge most characterized transporters with respect biochemical, physiological, activities. We further discuss localizations transporters, their substrate specificities, need for same context development.

Язык: Английский

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The birth of a giant: evolutionary insights into the origin of auxin responses in plants

The EMBO Journal, Год журнала: 2023, Номер 42(6)

Опубликована: Фев. 14, 2023

Abstract The plant signaling molecule auxin is present in multiple kingdoms of life. Since its discovery, a century research has been focused on action as phytohormone. In land plants, regulates growth and development through transcriptional non‐transcriptional programs. Some the molecular mechanisms underlying these responses are well understood, mainly Arabidopsis . Recently, availability genomic transcriptomic data green lineages, together with phylogenetic inference, provided basis to reconstruct evolutionary history some components involved biology. this review, we follow trajectory that allowed become “giant” biology by focusing bryophytes streptophyte algae. We consider biosynthesis, transport, physiological, responses, evidence supporting role chemical messenger for communication within ecosystems. Finally, emphasize functional validation predicted orthologs will shed light conserved properties among streptophytes.

Язык: Английский

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RAF-like protein kinases mediate a deeply conserved, rapid auxin response

Cell, Год журнала: 2023, Номер 187(1), С. 130 - 148.e17

Опубликована: Дек. 20, 2023

The plant-signaling molecule auxin triggers fast and slow cellular responses across land plants algae. nuclear pathway mediates gene expression controls growth development in plants, but this is absent from algal sister groups. Several components of rapid have been identified Arabidopsis, it unknown if these are part a conserved mechanism. We recently fast, proteome-wide phosphorylation response to auxin. Here, we show that occurs 5 plant species converges on core group shared targets. found physiological the same rapidly accelerated fibrosarcoma (RAF)-like protein kinases as central mediators auxin-triggered species. Genetic analysis connects kinase both response, thus identifying an ancient mechanism for green lineage.

Язык: Английский

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TIR1-produced cAMP as a second messenger in transcriptional auxin signalling

Nature, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Язык: Английский

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An LRR receptor kinase controls ABC transporter substrate preferences during plant growth-defense decisions

Current Biology, Год журнала: 2023, Номер 33(10), С. 2008 - 2023.e8

Опубликована: Май 1, 2023

Язык: Английский

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Distinct functions of TIR1 and AFB1 receptors in auxin signaling

Molecular Plant, Год журнала: 2023, Номер 16(7), С. 1117 - 1119

Опубликована: Июль 1, 2023

Auxin is the major plant hormone regulating growth and development (Friml, 2022Friml J. Fourteen Stations of Auxin.Cold Spring Harbor Perspect. Biol. 2022; 14a039859https://doi.org/10.1101/cshperspect.a039859Crossref PubMed Scopus (28) Google Scholar). Forward genetic approaches have identified components auxin signaling established canonical mechanism mediating transcriptional thus developmental reprogramming in Arabidopsis thaliana. In this textbook view, TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) proteins are receptors, which act as F-box subunits determining substrate specificity Skp1–Cullin1–F box protein (SCF) type E3 ubiquitin ligase complex. acts a "molecular glue," increasing affinity between TIR1/AFBs Auxin/Indole-3-Acetic Acid (Aux/IAA) repressors. Subsequently, Aux/IAAs ubiquitinated degraded, releasing transcription factors from their repression making them free to mediate response genes (Yu et al., 2022Yu Z. Zhang F. Friml Ding signaling: Research advances over past 30 years.J. Integr. Plant 64: 371-392https://doi.org/10.1111/jipb.13225Crossref (54) Nonetheless, accumulating evidence suggests existence rapid, non-transcriptional responses downstream such auxin-induced cytosolic calcium (Ca2+) transients, plasma membrane depolarization, apoplast alkalinization, all converging on process root inhibition gravitropism (Li 2022Li L. Gallei M. Bending auxin: fast acid for tropisms.Trends Sci. 27: 440-449https://doi.org/10.1016/j.tplants.2021.11.006Abstract Full Text PDF (16) Particularly, these rapid mostly contributed by predominantly AFB1, while long-term mediated mainly nuclear TIR1 AFB2–AFB5 (Prigge 2020Prigge M.J. Platre Kadakia N. Y. Greenham K. Szutu W. Pandey B.K. Bhosale R.A. Bennett Busch al.Genetic analysis TIR1/AFB receptors reveals both overlapping specialized functions.Elife. 2020; 9e54740https://doi.org/10.7554/eLife.54740Crossref (79) Scholar; Li 2021Li Verstraeten I. Roosjen Takahashi Rodriguez Merrin Chen Shabala Smet Ren H. al.Cell surface intracellular signalling H(+) fluxes growth.Nature. 2021; 599: 273-277https://doi.org/10.1038/s41586-021-04037-6Crossref (70) Serre 2021Serre N.B.C. Kralík D. Yun P. Slouka S. Fendrych AFB1 controls through depolarization thaliana root.Native Plants. 7: 1229-1238https://doi.org/10.1038/s41477-021-00969-zCrossref (30) How conducts auxin-triggered how it different remains elusive. Here, we compare roles modulating subcellular localization testing ability when part minimal circuit reconstituted yeast. One prominent difference localization. primarily localizes nucleus cytoplasm (Figure 1A Supplemental Figure 1) To test whether specific necessary prerequisite function either or responses, fused Venus report gene combined with exporting signal (NES) (NLS) at C termini (TIR1–NES–Venus) (AFB1–NLS–Venus), respectively. We showed that majority TIR1–NES–Venus shifted cytosol, AFB1–NLS–Venus concentrates 1B 1). characterize importance versus auxin-mediated transcription, tested if they can rescue mutant phenotype sustained after treatment 6 days. introduced cytosolic-localized into tir1 background found constructs were able completely restore sensitivity (Figures 1C 1D Figures 2). This be explained following: (i) residual present sufficient conduct full activity, (ii) may still degrade Aux/IAAs, ARFs inhibition. Besides, nuclear-localized afb2 mutants but did not observe any auxin-insensitive inhibition, gravitropism, lateral formation, hair elongation implies even localized nucleus, cannot functionally replace its regulation related development. The seems receptor effects Therefore, our mistargeted versions afb1 effect microfluidic vRootchip system. targeted could no longer 1E). On other hand, TIR1, despite being 1F). supplement function. observations show distinct functional properties unrelated confirm this, made use pathway reconstructed yeast (Pierre-Jerome 2014Pierre-Jerome E. Jang S.S. Havens K.A. Nemhauser J.L. Klavins Recapitulation forward yeast.Proc. Natl. Acad. USA. 2014; 111: 9407-9412https://doi.org/10.1073/pnas.1324147111Crossref (63) system, only regardless localization, was monitored fluorescence intensity P3_Venus reporter 1G). understand why signaling, form an SCF complex using two-hybrid approach. Only interact CUL1 (Cullin1), key component 1H). consistent available coimmunoprecipitation/mass spectrometry data, where detected TIR1; however, extremely weak interaction (Supplemental 3) 2015Yu Moss B.L. Bargmann B.O.R. Wang R. Prigge Estelle Untethering increases stability inhibits response.Native 2015; 114030https://doi.org/10.1038/nplants.2014.30Crossref reason does might natural mutation glutamic 8 site absence will prevent conducting failing Aux/IAA degradation Scholar) also explains artificially Our imply essential responses. requires further clarification role well ubiquitination A recent study revealed novel producing 3′,5′-cyclic adenosine monophosphate (cAMP), second messenger animals. Though activity specifically important (Qi 2022Qi Kwiatkowski Hoermayer Sinclair Zou Del Genio C.I. Kubeš M.F. Napier Jaworski al.Adenylate cyclase plants.Nature. 611: 133-138https://doi.org/10.1038/s41586-022-05369-7Crossref (27) Scholar), possible AFB1-mediated cAMP production cytosol is. However, adenylate contributes remain unknown. summary, demonstrated functions, slow divergence not, simply due differential receptors. slow/transcriptional independent predominant contrast, necessitates themselves. Furthermore, mediates without forming machinery, leaving exciting topic future investigations. project funded European Council Advanced Grant (ETAP-742985).

Язык: Английский

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