RAF-like protein kinases mediate a deeply conserved, rapid auxin response

Cell, Год журнала: 2023, Номер 187(1), С. 130 - 148.e17

Опубликована: Дек. 20, 2023

The plant-signaling molecule auxin triggers fast and slow cellular responses across land plants algae. nuclear pathway mediates gene expression controls growth development in plants, but this is absent from algal sister groups. Several components of rapid have been identified Arabidopsis, it unknown if these are part a conserved mechanism. We recently fast, proteome-wide phosphorylation response to auxin. Here, we show that occurs 5 plant species converges on core group shared targets. found physiological the same rapidly accelerated fibrosarcoma (RAF)-like protein kinases as central mediators auxin-triggered species. Genetic analysis connects kinase both response, thus identifying an ancient mechanism for green lineage.

Язык: Английский

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The birth of a giant: evolutionary insights into the origin of auxin responses in plants

The EMBO Journal, Год журнала: 2023, Номер 42(6)

Опубликована: Фев. 14, 2023

Abstract The plant signaling molecule auxin is present in multiple kingdoms of life. Since its discovery, a century research has been focused on action as phytohormone. In land plants, regulates growth and development through transcriptional non‐transcriptional programs. Some the molecular mechanisms underlying these responses are well understood, mainly Arabidopsis . Recently, availability genomic transcriptomic data green lineages, together with phylogenetic inference, provided basis to reconstruct evolutionary history some components involved biology. this review, we follow trajectory that allowed become “giant” biology by focusing bryophytes streptophyte algae. We consider biosynthesis, transport, physiological, responses, evidence supporting role chemical messenger for communication within ecosystems. Finally, emphasize functional validation predicted orthologs will shed light conserved properties among streptophytes.

Язык: Английский

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Phylogenomic insights into the first multicellular streptophyte

Current Biology, Год журнала: 2024, Номер 34(3), С. 670 - 681.e7

Опубликована: Янв. 21, 2024

Streptophytes are best known as the clade containing teeming diversity of embryophytes (land plants).1Wickett N.J. Mirarab S. Nguyen N. Warnow T. Carpenter E. Matasci Ayyampalayam Barker M.S. Burleigh J.G. Gitzendanner M.A. et al.Phylotranscriptomic analysis origin and early diversification land plants.Proc. Natl. Acad. Sci. USA. 2014; 111: E4859-E4868Crossref PubMed Scopus (883) Google Scholar,2One Thousand Plant Transcriptomes InitiativeOne thousand plant transcriptomes phylogenomics green plants.Nature. 2019; 574: 679-685Crossref (856) Scholar,3Donoghue P.C.J. Harrison C.J. Paps J. Schneider H. The evolutionary emergence plants.Curr. Biol. 2021; 31: R1281-R1298Abstract Full Text PDF (0) Scholar,4Puttick M.N. Morris J.L. Williams T.A. Cox Edwards D. Kenrick P. Pressel Wellman C.H. Pisani Donoghue interrelationships plants nature ancestral embryophyte.Curr. 2018; 28: 733-745.e2Abstract (288) Scholar Next to however a range freshwater terrestrial algae that bear important information on key traits plants. Among these, Klebsormidiophyceae stand out. Thriving in diverse environments—from mundane (ubiquitous occurrence tree barks rocks) extreme (from Atacama Desert Antarctic)—Klebsormidiophyceae can exhibit filamentous body plans display remarkable resilience colonizers habitats.5Karsten U. Holzinger A. Light, temperature, desiccation effects photosynthetic activity, drought-induced ultrastructural changes alga Klebsormidium dissectum (Streptophyta) from high alpine soil crust.Microb. Ecol. 2012; 63: 51-63Crossref (97) Scholar,6Holzinger Lütz C. Karsten Desiccation stress causes structural alterations aeroterrestrial crenulatum (Klebsormidiophyceae, Streptophyta) isolated an Alpine crust.J. Phycol. 2011; 47: 591-602Crossref (71) Currently, lack robust phylogenetic framework for hampers our understanding history these traits. Here, we conducted phylogenomic utilizing advanced models counteract systematic biases. We sequenced 24 new combined them with 14 previously published genomic transcriptomic datasets. Using built 845 loci sophisticated mixture models, establish framework, dividing six distinct genera novel three-order system, deep divergence more than 830 million years ago. Our reconstructions states suggest (1) multiple transitions between terrestrial-aquatic habitats, stem Klebsormidiales having conquered earlier embryophytes, (2) plan last common ancestor was multicellular, probability it whereas sarcinoids unicells likely derived states. provide evidence first multicellular streptophytes lived about billion

Язык: Английский

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An auxin research odyssey: 1989–2023

The Plant Cell, Год журнала: 2024, Номер 36(5), С. 1410 - 1428

Опубликована: Фев. 21, 2024

Abstract The phytohormone auxin is at times called the master regulator of plant processes and has been shown to be a central player in embryo development, establishment polar axis, early aspects seedling growth, as well growth organ formation during later stages development. Plant Cell key, since inception journal, developing an understanding biology. Auxin-regulated control accomplished by both changes levels active hormones sensitivity tissues these concentration changes. In this historical review, we chart research it progressed key areas highlight role played scientific developments. We focus on auxin-responsive genes, transcription factors, reporter constructs, perception, signal transduction processes. Auxin metabolism discussed from development tryptophan auxotrophic mutants, molecular biology conjugate hydrolysis, indole-3-butyric acid transport, steps indole-3-acetic biosynthesis, catabolism, transport. This progress leads expectation more comprehensive systems spatial temporal regulation cellular

Язык: Английский

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Enigmatic role of auxin response factors in plant growth and stress tolerance

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Июнь 10, 2024

Abiotic and biotic stresses globally constrain plant growth impede the optimization of crop productivity. The phytohormone auxin is involved in nearly every aspect development. Auxin acts as a chemical messenger that influences gene expression through short nuclear pathway, mediated by family specific DNA-binding transcription factors known Response Factors (ARFs). ARFs thus act effectors response translate signals into regulation responsive genes. Since initial discovery first ARF Arabidopsis, advancements genetics, biochemistry, genomics, structural biology have facilitated development models elucidating action their contributions to generating responses. Yet, significant gaps persist our understanding despite these endeavors. Unraveling functional roles regulating stress response, alongside genetic molecular mechanisms, still its nascent phase. Here, we review recent research outcomes on ARFs, detailing involvement leaf, flower, root organogenesis development, well responses corresponding regulatory mechanisms: including patterns, characterization, transcriptional, post-transcriptional post- translational across diverse conditions. Furthermore, delineate unresolved questions forthcoming challenges research.

Язык: Английский

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To bind or not to bind: how AUXIN RESPONSE FACTORs select their target genes

Journal of Experimental Botany, Год журнала: 2023, Номер 74(22), С. 6922 - 6932

Опубликована: Июль 11, 2023

Abstract Most plant growth and development processes are regulated in one way or another by auxin. The best-studied mechanism which auxin exerts its regulatory effects is through the nuclear pathway (NAP). In this pathway, Auxin Response Factors (ARFs) transcription factors that ultimately determine genes become binding to specific DNA sequences. ARFs have primarily been studied Arabidopsis thaliana, but recent studies other species revealed family-wide specificities for different minimal functional system of NAP system, consisting a duo competing A B classes. review, we provide an overview key aspects ARF such as response elements (TGTCNN) tandem repeat motifs, consider how structural biology vitro help us understand preferences. We also highlight some related regulation levels inside cell, may alter profile tissues. finally emphasize need study systems fundamental function, characterize algal evolved, cutting-edge techniques can increase our understanding ARFs, remaining questions only be answered biology.

Язык: Английский

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Phylogeny and evolution of streptophyte algae

Annals of Botany, Год журнала: 2024, Номер 134(3), С. 385 - 400

Опубликована: Июнь 4, 2024

Abstract The Streptophyta emerged about a billion years ago. Nowadays, this branch of the green lineage is most famous for one its clades, land plants (Embryophyta). Although Embryophyta make up major share species numbers in Streptophyta, there diversity probably >5000 streptophyte algae that form paraphyletic grade next to plants. Here, we focus on deep divergences gave rise streptophytes, hence particularly algae. Phylogenomic efforts have not only clarified position relative plants, but recent also begun unravel relationships and radiations within algal diversity. We illustrate how new phylogenomic perspectives changed our view evolutionary emergence key traits, such as intricate signalling networks are intertwined with multicellular growth chemodiverse hotbed from which they emerged. These traits biology were bequeathed their progenitors.

Язык: Английский

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Auxin and tryptophan trigger common responses in the streptophyte alga Penium margaritaceum

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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Evolutionary origins and functional diversification of Auxin Response Factors

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 30, 2024

The Auxin Response Factors (ARFs) family of transcription factors are the central mediators auxin-triggered transcriptional regulation. Functionally different classes extant ARFs operate as antagonistic auxin-dependent and -independent regulators. While part evolutionary trajectory to present auxin response functions has been reconstructed, it is unclear how emerged, early diversification led functionally proteins. Here, we use in silico vivo analyses revisit molecular events that origin subsequent evolution ARFs. We reveal shared from preexisting domains, uncovering a protein fold homologous ARF DNA-binding conserved eukaryotic chromatin regulator. Building on this, reconstruct complete history ARFs, including divergence leading appearance defining main targets for their functional diversification. derive emergence nuclear signalling pathway.

Язык: Английский

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ZmPILS6 is an auxin efflux carrier required for maize root morphogenesis

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(22)

Опубликована: Май 23, 2024

Plant root systems play a pivotal role in plant physiology and exhibit diverse phenotypic traits. Understanding the genetic mechanisms governing growth development model plants like maize is crucial for enhancing crop resilience to drought nutrient limitations. This study focused on identifying characterizing ZmPILS6, an annotated auxin efflux carrier, as key regulator of various crown traits maize. ZmPILS6-modified roots displayed reduced network area suppressed lateral formation, which are desirable “steep, cheap, deep” ideotype. The research revealed that ZmPILS6 localizes endoplasmic reticulum plays vital controlling spatial distribution indole-3-acetic acid (IAA or “auxin”) primary roots. also demonstrated can actively IAA when expressed yeast. Furthermore, loss resulted significant proteome remodeling roots, particularly affecting hormone signaling pathways. To identify potential interacting partners weighted gene coexpression analysis was performed. Altogether, this contributes growing knowledge essential determinants morphogenesis, guiding agricultural improvement strategies.

Язык: Английский

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