Community Dynamics in Structure and Function of Honey Bee Gut Bacteria in Response to Winter Dietary Shift

mBio, Год журнала: 2022, Номер 13(5)

Опубликована: Авг. 29, 2022

Temperate honey bees (Apis mellifera) are challenged by low temperatures and abrupt dietary shifts associated with behavioral changes during winter. Case studies have revealed drastic turnover in the gut microbiota of winter bees, highlighted seasonal dominance a non-core bacterium Bartonella. However, neither biological consequence nor underlying mechanism this microbial is clear. In particular, we ask whether such profile related to shift possibly beneficial host microbiome? Here, integrated evidences from genomics, metagenomics, metabolomics three bee subspecies maintained at same locality northern China both diversity functional variations bacteria across seasons. Our results showed that Bartonella was shared all tested lineages. This change likely characterized greatly reduced pollen consumption accumulation metabolic waste due restricted excretion. expanded genomic capacity utilizing more diverse energy substrates, as converting wastes lactate ethanol into pyruvate, an source for self-utilization also other symbionts. Furthermore, only capable producing secreting tryptophan phenylalanine, whose products were detected guts, even though lacked relevant digestion enzymes. These thus suggested possible where might benefit supplementing them essential amino acids lacking protein shortage diet. IMPORTANCE The abilities survive adapt major food key traits enabled successful range expansion tropic temperate climate. new possibility may played important role survival severe condition. not adaptive diet but equipped provide nutrients substrates. overall host-bacterium probably favored natural selection, resulting consistent strategy varied Conversely, when hosts start forage again, core abundance returned their typical dominant status, completing annual turnover. study suggests hypothesis improve fitness bee, allowing explore climates.

Язык: Английский

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Physiological differences between wild and captive animals: a century-old dilemma

Journal of Experimental Biology, Год журнала: 2023, Номер 226(23)

Опубликована: Ноя. 30, 2023

ABSTRACT Laboratory-based research dominates the fields of comparative physiology and biomechanics. The power lab work has long been recognized by experimental biologists. For example, in 1932, Georgy Gause published an influential paper Journal Experimental Biology describing a series clever experiments that provided first empirical test competitive exclusion theory, laying foundation for field remains active today. At time, wrestled with dilemma conducting or field, ultimately deciding progress could be best achieved taking advantage high level control offered experiments. However, physiological often yield different, even contradictory, results when conducted versus settings. This is especially concerning Anthropocene, as standard laboratory techniques are increasingly relied upon to predict how wild animals will respond environmental disturbances inform decisions conservation management. In this Commentary, we discuss several hypothesized mechanisms explain disparities between biology field. We propose strategies understanding why these differences occur can use improve our animals. Nearly century beyond Gause's work, still know remarkably little about what makes captive different from ones. Discovering should important goal biologists future.

Язык: Английский

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The gut microbiome of solitary bees is mainly affected by pathogen assemblage and partially by land use

Environmental Microbiome, Год журнала: 2023, Номер 18(1)

Опубликована: Апрель 26, 2023

Pollinators, including solitary bees, are drastically declining worldwide. Among the factors contributing to this decline, bee pathogens and different land uses of relevance. The link between gut microbiome composition host health has been recently studied for social pollinators (e.g. honeybees), whereas information related bees is sparse. This work aimed at characterization Xylocopa augusti, Eucera fervens Lasioglossum attempted correlate microbial with presence load uses. Solitary were sampled in sites (i.e. a farm, natural reserve, an urban plant nursery) showing DNA was extracted from gut, 16S rRNA gene amplified sequenced. Eight pathogens, known spillover managed wild ones, quantified qPCR. results showed that core profile three significantly varied species. Pseudomonas found as major taxa all analyzed, Lactobacillus, Spiroplasma Sodalis second most abundant X. E. Lasioglossum, respectively. main detected qPCR Nosema ceranae, bombi Crithidia bombi, although differently species sampling sites. Most did not show any correlation use, apart Snodgrassella Nocardioides, higher abundances on less anthropized Conversely, strongly affected composition, Bifidobacterium, Apibacter, Serratia, abundance positively or negatively correlated load. Therefore, appear be factor shaping Argentina.

Язык: Английский

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Climate change is not just global warming: Multidimensional impacts on animal gut microbiota

Microbial Biotechnology, Год журнала: 2023, Номер 16(9), С. 1736 - 1744

Опубликована: Май 29, 2023

Abstract Climate change has rapidly altered many ecosystems, with detrimental effects for biodiversity across the globe. In recent years, it become increasingly apparent that microorganisms live in and on animals can substantially affect host health physiology, structure function of these microbial communities be highly sensitive to environmental variables. To date, most studies have focused increasing mean temperature gut microbiota, yet other aspects climate are also shifting, including variation, seasonal dynamics, precipitation frequency severe weather events. This array pressures might interact complex non‐intuitive ways impact microbiota consequently alter animal fitness. Therefore, understanding impacts requires a consideration multiple types stressors their interactive microbiota. Here, we present an overview some major findings research climatic gut. Although ample evidence now accumulated shifts important hosts, much less work been conducted variables interactions. We provide recommendations additional needed mechanistically link

Язык: Английский

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Symbiotic bacteria and fungi proliferate in diapause and may enhance overwintering survival in a solitary bee

The ISME Journal, Год журнала: 2024, Номер 18(1)

Опубликована: Янв. 1, 2024

Host-microbe interactions underlie the development and fitness of many macroorganisms, including bees. Whereas social bees benefit from vertically transmitted gut bacteria, current data suggests that solitary bees, which comprise vast majority species diversity within lack a highly specialized microbiome. Here, we examine composition abundance bacteria fungi throughout complete life cycle ground-nesting bee Anthophora bomboides standfordiana. In contrast to expectations, immature stages maintain distinct core microbiome consisting Actinobacterial genera (Streptomyces, Nocardiodes) fungus Moniliella spathulata. Dormant (diapausing) larval hosted most abundant distinctive fungi, attaining 33 52 times their initial copy number, respectively. We tested two adaptive hypotheses regarding microbial functions for diapausing First, using isolated found Streptomyces brood cells inhibited growth multiple pathogenic filamentous suggesting role in pathogen protection during overwintering, when face high pressure. Second, sugar alcohol changed tandem with major changes fungal abundance, links cold tolerance or overwintering biology. find A. hosts conserved may provide key advantages through diapause, raises question how this is maintained faithfully between generations. Our results suggest focus on microbiomes mature active insect developmental overlook stage-specific symbionts contributions host dormancy.

Язык: Английский

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Environmental and microhabitat influences on microbiota of snow-active Collembola in sub-zero temperatures

Applied Soil Ecology, Год журнала: 2025, Номер 208, С. 105998 - 105998

Опубликована: Март 2, 2025

Язык: Английский

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Metacommunity theory for transmission of heritable symbionts within insect communities

Ecology and Evolution, Год журнала: 2019, Номер 10(3), С. 1703 - 1721

Опубликована: Дек. 2, 2019

Abstract Microbial organisms are ubiquitous in nature and often form communities closely associated with their host, referred to as the microbiome. The microbiome has strong influence on species interactions, but studies rarely take interactions between hosts into account, network interaction consider microbiomes. Here, we propose use metacommunity theory a framework unify research microbiomes host by considering insects microbes discretely defined “communities of communities” linked dispersal (transmission) through biotic interactions. We provide an overview effects heritable symbiotic bacteria insect how those subsequently thereby altering community. suggest multiple scenarios for integrating ecology demonstrate ways which employ parameterize models symbiont transmission quantitatively assess processes host‐associated microbial systems. Successfully incorporating microbiota community‐level is crucial step understanding importance

Язык: Английский

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Gut Microbiota of Drosophila subobscura Contributes to Its Heat Tolerance and Is Sensitive to Transient Thermal Stress

Frontiers in Microbiology, Год журнала: 2021, Номер 12

Опубликована: Май 6, 2021

The gut microbiota can contribute to host physiology leading an increase of resistance abiotic stress conditions. For instance, temperature has profound effects on ectotherms, and the role thermal tolerance ectotherms is a matter recent research. However, most these studies have been focused single static temperatures instead evaluating in wide range stressful temperatures. Additionally, there evidence supporting that sensitive environmental temperature, which induces changes its composition diversity. These evaluated acclimation (>2 weeks) microbiota, but we know little about impact transient diversity microbiota. Thus, investigated heat Drosophila subobscura by measuring conventional axenic flies exposed different (35, 36, 37, 38°C) estimating landscape for both treatments. Conventional mild conditions exhibited higher than flies, whereas at were no differences between flies. We also assessed taxonomical abundance, diversity, community structure comparing non-stressed (exposed 21°C) heat-stressed 34°C) from sexes. Bacterial indices, bacterial abundances, changed this response was sex-dependent. In general, our findings provide influences modifies structural levels. results demonstrate contributes highly stress, could important consequences fitness, population risk extinction, vulnerability current future climatic

Язык: Английский

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Thank you for biting: dispersal of beneficial microbiota through 'antagonistic' interactions

Trends in Microbiology, Год журнала: 2022, Номер 30(10), С. 930 - 939

Опубликована: Апрель 5, 2022

Язык: Английский

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Cross-talk between low temperature and other environmental factors

Current Opinion in Insect Science, Год журнала: 2024, Номер 63, С. 101193 - 101193

Опубликована: Март 13, 2024

Язык: Английский

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