Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Ноя. 20, 2024
Over the past two decades, gut microbiota has demonstrated unprecedented potential in human diseases and health. The early life is crucial for later health outcomes. This study aims to reveal knowledge collaboration network, research hotspots, explore emerging trends fields of infant microbiome using bibliometric analysis.
Язык: Английский
Frontiers in Immunology, Год журнала: 2025, Номер 15
Опубликована: Янв. 14, 2025
Human milk oligosaccharides (HMOs) are abundant, diverse and complex sugars present in human breast milk. HMOs well-characterized barriers to microbial infection by modulating the microbiome they also thought be nutritionally beneficial infant. The structural variety of over 200 HMOs, including neutral, fucosylated sialylated forms, allows them interact with immune system various ways. Clinically, impact allergic diseases, reducing autoimmune inflammatory responses, offer support preterm infant health. This review examines HMO composition associated immunomodulatory effects, interactions cell receptors gut-associated responses. These properties highlight potential for use early stage development as novel immunotherapeutics. research is rapidly evolving promises innovative treatments immune-related conditions improved health outcomes.
Язык: Английский
Процитировано
1
Food Chemistry, Год журнала: 2025, Номер 473, С. 142821 - 142821
Опубликована: Янв. 7, 2025
Язык: Английский
Процитировано
0
World Journal of Clinical Pediatrics, Год журнала: 2025, Номер 14(2)
Опубликована: Март 18, 2025
BACKGROUND Human milk oligosaccharides (HMOs) are bioactive components of breast with diverse health benefits, including shaping the gut microbiota, modulating immune system, and protecting against infections. HMOs exhibit dynamic secretion patterns during lactation, influenced by maternal genetics environmental factors. Their direct indirect antimicrobial properties have garnered significant research interest. However, a comprehensive understanding dynamics their correlation efficacy remains underexplored. AIM To synthesize current evidence on lactation evaluate roles bacterial, viral, protozoal pathogens. METHODS A systematic search PubMed, Scopus, Web Science, Cochrane Library focused studies investigating natural synthetic HMOs, dynamics, properties. Studies involving human, animal, in vitro models were included. Data HMO composition, temporal patterns, mechanisms action extracted. Quality assessment was performed using validated tools appropriate for study design. RESULTS total 44 included, encompassing research. exhibited 2′-fucosyllactose (2′-FL) lacto-N-tetraose peaking early declining over time, while 3-fucosyllactose (3-FL) increased later stages. demonstrated through pathogen adhesion inhibition, biofilm disruption, enzymatic activity impairment. Synthetic bioengineered 2′-FL 3-FL, structurally functionally comparable to effectively inhibiting pathogens such as Pseudomonas aeruginosa , Escherichia coli Campylobacter jejuni . Additionally, synergistic effects antibiotics, enhancing resistant CONCLUSION vital defense, supporting infant targeting various Both hold potential therapeutic applications, particularly nutrition adjuncts antibiotics. Further research, clinical trials, is essential address gaps knowledge, validate findings, explore broader applicability improving neonatal health.
Язык: Английский
Процитировано
0
Journal of Dairy Science, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Free monosaccharides are important carbohydrates in milk, providing both basic and bioactive nutritional benefits; however, the content composition of free milk from different species still not well understood. The aim this study was to develop a highly sensitive accurate liquid chromatography (LC)-MS method for precise quantification 8 species, including human, cow, goat, sheep, yak, camel, horse, donkey. chromatographic conditions MS parameters were systematically optimized ensure high resolution, minimal matrix effects, low detection limits all target monosaccharides. validated with excellent linearity (R2 > 0.996), recovery rates (94.18%-115.02%), CV (<10%), demonstrating robustness across various concentrations. compositional analysis revealed significant interspecies differences monosaccharide profiles. Human uniquely enriched glucose (4,262.98 ± 246.49 ng/mL) fucose (1,024.80 61.82 ng/mL). In contrast, ruminant such as cow sheep exhibited levels galactose (1,803.56 94.63 ng/mL 1,230.31 52.33 ng/mL, respectively) mannose (375.24 16.27 55.81 3.76 respectively). Principal component complementary stacked bar chart effectively visualized clustering relative distribution among highlighting their metabolic functional diversity. This provides novel insights into biological roles evolutionary significance developed LC-MS offers robust tool advancing our understanding its implications neonatal nutrition, dairy product innovation, human health.
Язык: Английский
Процитировано
0
Nutrients, Год журнала: 2024, Номер 16(9), С. 1287 - 1287
Опубликована: Апрель 25, 2024
Human milk oligosaccharides (HMOs) are complexes that play a crucial role in shaping the early-life gut microbiota. This study intends to explore whether HMO patterns associated with microbiota of infants. We included 96 Chinese breastfeeding mother–infant dyads. Breast and infant faecal samples were collected tested. With 2′-fucosyllactose, difucosyllactose, lacto-N-fucopentaose-I as biomarkers, we divided mothers into secretor non-secretor groups. extracted using principal component analysis. The majority (70.7%) categorised five different identified. After adjustment, infants exhibited lower relative abundance Bifidobacterium bifidum (β = −0.245, 95%CI: −0.465~−0.025). An pattern characterised by high levels 3-fucosyllactose, lacto-N-fucopentaose-III, lacto-N-neodifucohexaose-II was positively breve (p 0.014), while lacto-N-neotetraose, 6′-sialyllactose, sialyllacto-N-tetraose-b negatively 0.027). monofucosyl-lacto-N-hexaose-III monofucosyl-lacto-N-neohexaose dentium 0.025) < 0.001), respectively. suggests from mature breast certain breastfed
Язык: Английский
Процитировано
3
JACS Au, Год журнала: 2024, Номер 4(11), С. 4496 - 4506
Опубликована: Ноя. 2, 2024
Among human milk oligosaccharides (HMOs), linear HMOs are synthesized through mature but varied routes. Although branched can be by chemical, enzymatic, or chemoenzymatic methods, these methods cannot easily applied to the synthesis of asymmetric multiantennary oligosaccharides. Herein, we developed a controllable method synthesize biantennary HMOs. In our synthetic route, GlcNAcβ1,3(GlcN3β1,6)Glaβ1,4Glc was first chemically as core tetrasaccharide, which contains β1,6GlcN3 "stop" sugar in transferase-catalyzed glycosylation. The desired sugars at GlcNAcβ1–3Gal arm assembled using galactosyltransferase, N-acetylglucosaminyltransferase, and fucosyltransferase. Then, Staudinger reduction acetylation were used transform GlcN3 GlcNAc assemble initiating "go" process. By manipulating glycosylations, 22 natural synthesized.
Язык: Английский
Процитировано
3
Nutrients, Год журнала: 2024, Номер 16(17), С. 2858 - 2858
Опубликована: Авг. 26, 2024
Early-life nutrition significantly impacts vaccination efficacy in infants, whose immune response to vaccines is weaker compared adults. This study investigated female C57Bl/6JOlaHsd mice (6 weeks old) fed diets with 0.7% galacto-oligosaccharides (GOS)/long-chain fructo-oligosaccharides (lcFOS) (9:1), 0.3% human milk oligosaccharides (HMOS), or a combination (GFH) for 14 days prior and during vaccination. Delayed-type hypersensitivity (DTH) was measured by assessing ear swelling following an intradermal challenge. Influvac-specific IgG1 IgG2a levels were assessed using ELISAs, while splenic T B lymphocytes analyzed frequency activation via flow cytometry. Additionally, cytokine production evaluated murine splenocytes co-cultured influenza-loaded dendritic cells. Mice on the GFH diet showed enhanced DTH (p < 0.05), increased serological levels, significant rise memory (CD27+ B220+ CD19+). GFH-fed also exhibited more activated Th1 cells (CD69+ CXCR3+ CD4+) higher IFN-γ after ex vivo restimulation 0.05). These findings suggest that GOS/lcFOS HMOS, particularly combination, enhance vaccine responses improving cells, IgG production, cell activation, supporting potential use of these prebiotics infant formula better early-life development.
Язык: Английский
Процитировано
1
Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Ноя. 20, 2024
Over the past two decades, gut microbiota has demonstrated unprecedented potential in human diseases and health. The early life is crucial for later health outcomes. This study aims to reveal knowledge collaboration network, research hotspots, explore emerging trends fields of infant microbiome using bibliometric analysis.
Язык: Английский
Процитировано
0