Development of Sheep Intestinal Organoids for Studying Deoxynivalenol-Induced Toxicity
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 955 - 955
Опубликована: Янв. 23, 2025
Sheep
are
an
important
livestock
species
whose
gastrointestinal
tract
is
essential
for
overall
health.
Feed
contaminants
such
as
bacterial
toxins
and
mycotoxins
severely
damage
the
sheep
intestine,
yet
mechanisms
remain
mostly
elusive
partially
due
to
lack
of
physiologically
relevant
in
vitro
models.
Here,
we
investigated
molecular
underlying
deoxynivalenol
(DON)-induced
toxicity
by
developing
intestinal
organoids
from
isolated
crypts
Hu
sheep.
The
had
a
central
lumen
monolayer
epithelium,
could
be
continuously
passaged,
cryopreserved,
resuscitated.
Histological
transcriptomic
analysis
showed
that
recapitulate
cell
lineages
gene
expression
characteristics
original
tissues.
Statistical
indicated
DON
exposure
significantly
inhibited
organoid
formation
efficiency,
well
proliferation
activity
cells.
RNA-seq
Western
blotting
further
revealed
induces
inhibiting
PI3K/AKT/GSK3β/β-catenin
signaling
pathway.
Our
study
provides
novel
example
application
studies
reveals
pathway
involved
DON-induced
sheep,
which
great
significance
improving
mitigation
strategies
DON.
Язык: Английский
Novel lactobacillus consortium for effective zearalenone adsorption and biodegradation
International Biodeterioration & Biodegradation,
Год журнала:
2024,
Номер
194, С. 105889 - 105889
Опубликована: Авг. 18, 2024
Язык: Английский
Evaluation of Dietary Bioactive Agents Against Aflatoxin B1 and Ochratoxin A-Induced Duodenal Toxicity in Rats
Foods,
Год журнала:
2025,
Номер
14(10), С. 1793 - 1793
Опубликована: Май 18, 2025
Aflatoxin
B1
(AFB1)
and
Ochratoxin
A
(OTA)
are
two
of
the
most
potent
mycotoxins,
recognized
for
their
severe
toxicity.
In
recent
years,
consumption
bioactive
substances
has
proven
to
be
a
valuable
ally
in
combating
harmful
effects
on
human
health.
For
this
purpose,
study
evaluates
protective
fermented
whey
(FW)
pumpkin
(P),
as
functional
ingredients
bread,
duodenum
tissue
against
sub-chronic
toxicity
induced
by
AFB1
OTA.
Nine
groups
male
female
Wistar
rats
(n
=
5
per
sex/group)
were
exposed
different
combinations
AFB1,
OTA,
FW,
P
28
days.
The
gene
expression
apoptotic
antioxidant
markers,
including
p53,
Bax,
Hmox1,
NF-κB,
occludin,
was
measured
quantitative
real-time
PCR
(RT-qPCR).
+
OTA
exposure
led
an
increased
p53
with
downregulation
Bax
Hmox1.
Occludin
expression,
which
supports
tight
junction
integrity,
remained
largely
unaffected.
Supplementation
FW
modulated
favorably,
offering
protection
These
components
effectively
mitigated
oxidative
stress
apoptosis
duodenal
tissue.
Notably,
results
indicate
that
not
sex-dependent.
findings
highlight
potential
toxic
vivo.
Язык: Английский
Mycotoxins and secondary metabolites from fruit postharvest pathogenic Penicillium species
Food Chemistry,
Год журнала:
2025,
Номер
unknown, С. 144880 - 144880
Опубликована: Май 1, 2025
Язык: Английский
Magnetic aggregation-induced emission of bifunctional nanoparticles for enhanced double-color lateral flow immunoassay of fumonisin B1 and T-2 toxin
Food Chemistry,
Год журнала:
2025,
Номер
488, С. 144898 - 144898
Опубликована: Май 23, 2025
Язык: Английский
Innovative probiotic fermentation approach for zearalenone detoxification in dried distiller’s grains
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Июнь 2, 2025
Zearalenone
(ZEN)
contamination
in
dried
distiller’s
grains
and
solubles
(DDGS)
poses
serious
health
risks
economic
losses
animal
farming.
This
study
aimed
to
evaluate
the
effectiveness
of
probiotic
fermentation
using
Lactobacillus
plantarum
CN1
detoxifying
ZEN
optimizing
conditions
for
maximum
efficiency.
L.
CN1,
identified
with
99%
genetic
homology,
was
used
DDGS
fermentation.
The
detoxification
mechanism
analyzed
through
adsorption
assays,
post-heat
treatment
effects,
scanning
electron
microscopy
(SEM).
removal
assessed
over
72
h
under
various
conditions,
including
bacterial
concentration,
temperature,
pH
optimization.
results
showed
that
achieved
a
rate
69%
within
h,
an
optimized
efficiency
75.6%
at
4
×
10
9
CFU/mL.
Over
60%
adsorbed
by
cell
wall,
while
supernatant
intracellular
fluid
remained
below
5%.
Scanning
(SEM)
analysis
highlighted
structural
changes
cells,
particularly
elongation
thinning,
more
pronounced
damage
observed
following
heat
treatment.
These
modifications
may
explain
varying
efficiencies
observed.
Heat
treatment,
autoclaving,
significantly
enhanced
82.9%,
whereas
acid
alkali
treatments
reduced
it.
Fermentation
also
improved
nutritional
quality
DDGS,
increasing
crude
protein
7.16%,
reducing
fiber
0.65%,
lowering
4.3.
findings
demonstrate
offers
promising,
cost-effective
strategy
mitigating
enhancing
quality.
Future
studies
should
explore
large-scale
applications
potential
multi-mycotoxin
further
improve
feed
safety.
Язык: Английский
Probiotic Bacteria as a Green Solution for Mitigating Zearalenone Toxicity and Pollution
Food Bioscience,
Год журнала:
2024,
Номер
unknown, С. 105593 - 105593
Опубликована: Ноя. 1, 2024
Язык: Английский
Curcumin Attenuates Fumonisin B1-Induced Cell Apoptosis by Upregulating Mir-1249 Expression to Inhibit the Ire1/Mkk7/Jnk/Caspase3
Опубликована: Янв. 1, 2024
Fumonisin
B1
(FB1)
is
a
toxic
metabolite
produced
by
fungi
of
the
Fusarium
genus.
FB1
common
contaminant
grains
and
their
derived
feed
products,
posing
global
concerns
for
environmental
pollution
food
safety.
Curcumin
(Cur),
natural
polyphenolic
compound,
has
anti-inflammatory,
antioxidant,
free
radical
scavenging
properties.
Although
studies
have
explored
apoptotic
mechanisms
FB1,
underlying
mitigation
FB1-induced
toxicity
Cur
are
not
fully
understood,
limiting
its
potential
application
as
novel
additive
to
prevent
toxicity.
In
this
study,
porcine
kidney
cells
(PK-15)
were
used
experimental
model,
utilizing
mRNA
miRNA
transcriptome
technologies.
The
results
revealed
that
upregulated
miR-1249
inhibits
target
gene
Ern1
in
PK-15
cells,
thereby
suppressing
IRE1/MKK7/JNK/CASPASE3
endoplasmic
reticulum
(ER)
stress
pathway
alleviating
cell
apoptosis.
Cell
transfection
experiments
confirmed
effectively
attenuated
apoptosis
induced
ER
following
with
inhibitor.
Similarly,
mimic
alleviated
cells.
findings
indicate
mitigates
significantly
reduces
damage
Язык: Английский
Triclosan Induces Neurodevelopmental Toxicity Mediated by Circrna266/Mir-214 Regulatory Networks in Larval Zebrafish
Опубликована: Янв. 1, 2024
Язык: Английский
Gene recognition and role of foodomics in mycotoxin control: A review
Deleted Journal,
Год журнала:
2024,
Номер
3(1), С. 1857 - 1857
Опубликована: Дек. 9, 2024
Since
recognition
of
toxic
and
carcinogenic
aflatoxins
in
Brazilian
groundnut
meal
1960,
much
research
has
been
done
to
prevent
detoxify
foods
feeds,
identifying
a
variety
methods.
The
expanded
other
mycotoxins.
biotic
abiotic
factors
favoring
mycotoxin
contaminations
have
understood
through
experiments
under
laboratory
conditions
analysis
field
data.
However,
many
gaps
remain
the
knowledge
on
control
at
molecular
level
that
may
be
useful
addressing
mycotoxigenic
hazards.
Recognition
responsible
genes
hosts
fungi
omics
methods
applying
genomics,
transcriptomics,
proteomics,
metabolomics
understand
biosynthesis
open
new
avenues
interact
with
plant-fungi-bacteria
cross-talks,
apply
regulatory
mechanisms
biosynthesis,
explore
checks
controls
biosynthesis.
is
expected
generate
probable
biological
eliminate
foods.
current
requires
application
achieve
deeper
understanding,
aiming
for
next-generation
technologies.
This
review
examines
aflatoxins,
fusarium
toxins,
patulin
host-fungi
interactions
level.
Язык: Английский