On the ever‐growing functional versatility of the CRISPR‐Cas13 system

Microbial Biotechnology, Год журнала: 2024, Номер 17(2)

Опубликована: Фев. 1, 2024

CRISPR-Cas systems evolved in prokaryotes to implement a powerful antiviral immune response as result of sequence-specific targeting by ribonucleoproteins. One such consists an RNA-guided RNA endonuclease, known CRISPR-Cas13. In very recent years, this system is being repurposed different ways order decipher and engineer gene expression programmes. Here, we discuss the functional versatility CRISPR-Cas13 system, which includes ability for silencing, editing, tracking, nucleic acid detection translation regulation. This palette makes relevant tool broad field synthetic biology.

Язык: Английский

State of the art CRISPR-based strategies for cancer diagnostics and treatment

Biomarker Research, Год журнала: 2024, Номер 12(1)

Опубликована: Дек. 18, 2024

Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology is a groundbreaking and dynamic molecular tool for DNA RNA “surgery”. CRISPR/Cas9 the most widely applied system in oncology research. It major advancement genome manipulation due to its precision, efficiency, scalability versatility compared previous gene editing methods. has shown great potential not only targeting of oncogenes or genes coding immune checkpoint molecules, engineering T cells, but also epigenomic disturbances, which contribute cancer development progression. proven useful detecting genetic mutations, enabling large-scale screening involved tumor onset, progression drug resistance, speeding up highly targeted therapies tailored immunological profiles patient’s tumor. Furthermore, recently discovered Cas12 Cas13 systems have expanded Cas9-based applications, providing new opportunities diagnosis treatment cancer. In addition traditional cis-cleavage, they exhibit trans-cleavage activity, enables their use as sensitive specific diagnostic tools. Diagnostic platforms like DETECTR, employs enzyme, that cuts single-stranded reporters, SHERLOCK, uses Cas12, Cas13, specifically target cleave RNA, can be exploited speed advance oncological diagnostics. Overall, CRISPR platform improve diagnostics functionality safety engineered cellular medicines. Here, we will emphasize potentially transformative impact field treatments, prognostic approaches, highlight challenges raised by using newly introduced CRISPR-based therapy.

Язык: Английский

Advances in CRISPR-Cas systems for epigenetics

Progress in molecular biology and translational science, Год журнала: 2024, Номер unknown, С. 185 - 209

Опубликована: Янв. 1, 2024

Язык: Английский