Abstract
Aging
induces
systematic
changes
in
the
distribution
of
nucleosomes,
which
affect
gene
expression
programs.
Here
we
reconstructed
nucleosome
maps
based
on
cell‐free
DNA
(cfDNA)
extracted
from
blood
plasma
using
four
cohorts
people
different
ages.
We
show
that
nucleosomes
tend
to
be
separated
by
larger
genomic
distances
older
people,
and
age
correlates
with
repeat
length
(NRL).
Furthermore,
developed
first
aging
clock
cfDNA
nucleosomics.
Machine
learning
distance
distributions
allowed
predicting
person's
median
absolute
error
3–3.5
years.
Abstract
Advanced
age
is
the
main
common
risk
factor
for
cancer,
cardiovascular
disease
and
neurodegeneration.
Yet,
more
known
about
molecular
basis
of
any
these
groups
diseases
than
changes
that
accompany
ageing
itself.
Progress
in
research
was
slow
because
tools
predicting
whether
someone
aged
slowly
or
fast
(biological
age)
were
unreliable.
To
understand
as
a
to
develop
interventions,
field
needed
quantitative
measure;
clock
biological
age.
Over
past
decade,
number
predictors
utilising
DNA
methylation
have
been
developed,
referred
epigenetic
clocks.
While
they
appear
estimate
age,
it
remains
unclear
used
train
clocks
are
reflection
other
underlying
cellular
processes,
itself
involved
process.
The
precise
aspects
capture
remain
hidden
seem
vary
between
predictors.
Nonetheless,
use
has
opened
door
towards
studying
quantitatively,
new
applications,
such
forensics,
frequently.
In
this
review,
we
will
discuss
range
available,
their
strengths
weaknesses,
applicability
various
scientific
queries.
Nature Aging,
Год журнала:
2022,
Номер
3(1), С. 121 - 137
Опубликована: Дек. 19, 2022
Abstract
The
diversity
of
cell
types
is
a
challenge
for
quantifying
aging
and
its
reversal.
Here
we
develop
‘aging
clocks’
based
on
single-cell
transcriptomics
to
characterize
cell-type-specific
rejuvenation.
We
generated
transcriptomes
from
the
subventricular
zone
neurogenic
region
28
mice,
tiling
ages
young
old.
trained
single-cell-based
regression
models
predict
chronological
age
biological
(neural
stem
proliferation
capacity).
These
clocks
are
generalizable
independent
cohorts
other
regions
brains,
species.
To
determine
if
these
could
quantify
transcriptomic
rejuvenation,
datasets
two
interventions—heterochronic
parabiosis
exercise.
Aging
revealed
that
heterochronic
exercise
reverse
in
regions,
but
different
ways.
This
study
represents
first
development
high-resolution
data
demonstrates
their
application
Ageing Research Reviews,
Год журнала:
2023,
Номер
88, С. 101956 - 101956
Опубликована: Май 20, 2023
Epigenetic
aging
clocks
have
gained
significant
attention
as
a
tool
for
predicting
age-related
health
conditions
in
clinical
and
research
settings.
They
enabled
geroscientists
to
study
the
underlying
mechanisms
of
assess
effectiveness
anti-aging
therapies,
including
diet,
exercise
environmental
exposures.
This
review
explores
effects
modifiable
lifestyle
factors'
on
global
DNA
methylation
landscape,
seen
by
clocks.
We
also
discuss
through
which
these
factors
contribute
biological
provide
comments
what
findings
mean
people
willing
build
an
evidence-based
pro-longevity
lifestyle.
Abstract
Epigenetic
clocks
are
a
common
group
of
tools
used
to
measure
biological
aging—the
progressive
deterioration
cells,
tissues,
and
organs.
have
been
trained
almost
exclusively
using
blood‐based
but
there
is
growing
interest
in
estimating
epigenetic
age
less‐invasive
oral‐based
tissues
(i.e.,
buccal
or
saliva)
both
research
commercial
settings.
However,
differentiated
cell
types
across
body
exhibit
unique
DNA
methylation
landscapes
age‐related
alterations
the
methylome.
Applying
derived
from
estimate
may
introduce
biases.
We
tested
within‐person
comparability
five
tissue
types:
epithelial,
saliva,
dry
blood
spots,
buffy
coat
leukocytes),
peripheral
mononuclear
cells.
284
distinct
samples
83
individuals
aged
9–70
years.
Overall,
were
significant
differences
clock
estimates
versus
with
average
30
years
observed
some
clocks.
In
addition,
most
exhibited
low
correlation
despite
controlling
for
cellular
proportions
other
technical
factors.
Notably,
Skin
Blood
greatest
concordance
all
types,
indicating
its
ability
chronological
oral‐
tissues.
Our
findings
indicate
that
application
blood‐derived
not
yield
comparable
age,
highlighting
need
careful
consideration
type
when
age.
Foods,
Год журнала:
2025,
Номер
14(3), С. 347 - 347
Опубликована: Янв. 21, 2025
Bee
pollen
is
characterized
by
an
exceptional
diversity
and
abundance
of
micronutrients
bioactive
phytochemicals.
This
richness
remains
very
sparsely
investigated,
but
accumulating
evidence
strongly
supports
a
promising
future
for
bee
in
human
nutrition
medicine.
Epigenetic
regulation
among
the
most
compelling
biomedical
topics
that
remain
completely
untapped
derivative
research.
In
our
current
research,
we
identified
numerous
ubiquitous
compounds
are
consistently
present
this
matrix,
regardless
its
botanical
geographical
origins,
have
been
well
studied
documented
as
epigenetic
regulators
recent
years.
Given
relative
newness
both
research
studies
within
nutritional,
pharmaceutical,
medical
sciences,
review
aims
to
bridge
these
valuable
fields
advance
related
experimental
investigations.
To
best
knowledge,
first
work
has
aimed
comprehensively
investigate
modulatory
potential
compounds.
Our
findings
also
unveiled
several
intriguing
phenomena,
such
dual
effect
same
compound
depending
on
cellular
context
or
some
cross-generational
heritability
traits.
Although
whole
extract
still
lacking,
study
clearly
indicates
avenue
worth
further
We
hope
constitutes
foundational
cornerstone
investigations
Abstract
There
are
functional
benefits
to
exercise
in
muscle,
even
when
performed
late
life,
but
the
contributions
of
epigenetic
factors
late‐life
adaptation
poorly
defined.
Using
reduced
representation
bisulfite
sequencing
(RRBS),
ribosomal
DNA
(rDNA)
and
mitochondrial‐specific
examination
methylation,
targeted
high‐resolution
methylation
analysis,
DNAge™
aging
clock
analysis
with
a
translatable
model
voluntary
murine
endurance/resistance
training
(progressive
weighted
wheel
running,
PoWeR),
we
provide
evidence
that
may
mitigate
skeletal
muscle.
Late‐life
PoWeR
from
22–24
months
age
modestly
significantly
attenuates
an
age‐associated
shift
toward
promoter
hypermethylation.
The
muscle
old
mice
PoWeR‐trained
for
eight
weeks
was
approximately
younger
than
24‐month‐old
sedentary
counterparts,
which
represents
~8%
expected
lifespan.
These
data
molecular
basis
as
therapy
attenuate
aging.
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Авг. 2, 2024
Epigenetic
clocks
are
age
predictors
that
use
machine-learning
models
trained
on
DNA
CpG
methylation
values
to
predict
chronological
or
biological
age.
Increases
in
predicted
epigenetic
relative
(epigenetic
acceleration)
connected
aging-associated
pathologies,
and
changes
linked
canonical
aging
hallmarks.
However,
rely
training
data
from
bulk
tissues
whose
cellular
composition
with
Here,
we
found
human
naive
CD8+
T
cells,
which
decrease
frequency
during
aging,
exhibit
an
15–20
years
younger
than
effector
memory
cells
the
same
individual.
Importantly,
homogenous
isolated
individuals
of
different
ages
show
a
progressive
increase
age,
indicating
current
measure
two
independent
variables,
immune
cell
composition.
To
isolate
age-associated
intrinsic
changes,
created
clock,
IntrinClock,
did
not
change
among
10
types
tested.
IntrinClock
shows
robust
model
replicative
senescence
vitro
reversal
OSKM-mediated
reprogramming.
is
clock
resistant
potential
confounds
arising
age-dependent
can
robustly
multiple
contexts.
