bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 5, 2023
SUMMARY
Senescent
cells
drive
age-related
tissue
dysfunction
via
the
induction
of
a
chronic
senescence-associated
secretory
phenotype
(SASP).
The
cyclin-dependent
kinase
inhibitors
p21
Cip1
and
p16
Ink4a
have
long
served
as
markers
cellular
senescence.
However,
their
individual
roles
remain
incompletely
elucidated.
Thus,
we
conducted
comprehensive
examination
multiple
single-cell
RNA
sequencing
(scRNA-seq)
datasets
spanning
both
murine
human
tissues
during
aging.
Our
analysis
revealed
that
transcripts
demonstrate
significant
heterogeneity
across
distinct
cell
types
tissues,
frequently
exhibiting
lack
co-expression.
Moreover,
identified
tissue-specific
variations
in
SASP
profiles
linked
to
or
expression.
study
underscores
extraordinary
diversity
senescence
SASP,
emphasizing
these
phenomena
are
inherently
cell-
tissue-dependent.
few
factors
consistently
contribute
shared
“core”
SASP.
These
findings
highlight
need
for
more
nuanced
investigation
wide
array
biological
contexts.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(13), С. 7411 - 7411
Опубликована: Июль 5, 2024
Advancing
age
is
associated
with
several
age-related
diseases
(ARDs),
musculoskeletal
conditions
impacting
millions
of
elderly
people
worldwide.
With
orthopedic
contributing
towards
considerable
number
patients,
a
deeper
understanding
bone
aging
the
need
hour.
One
underlying
factors
cellular
senescence
and
its
secretory
phenotype
(SASP).
SASP
comprises
pro-inflammatory
markers,
cytokines
chemokines
that
arrest
cell
growth
development.
The
accumulation
over
years
leads
to
chronic
low-grade
inflammation
advancing
age,
also
known
as
inflammaging.
pathways
molecular
mechanisms
focused
on
inflammaging
are
currently
limited
but
increasingly
being
explored.
Most
genes,
involved
in
coincide
those
cancer
other
ARDs
like
osteoarthritis
(OA).
Thus,
exploring
these
using
techniques
sequencing,
identifying
combatting
them
most
suitable
approach
crucial
for
healthy
early
detection
ARDs.
Several
approaches
can
be
used
aid
regeneration
reduce
bone.
These
may
pharmacological,
non-pharmacological
lifestyle
interventions.
increasing
evidence
intricate
relationship
between
aging,
senescence,
ARDs,
anti-aging
strategies
marrow
(BM).
Biology,
Год журнала:
2022,
Номер
11(12), С. 1731 - 1731
Опубликована: Ноя. 29, 2022
Cellular
senescence
has
gained
increasing
attention
in
the
field
of
aging
research.
Senescent
cells
have
been
implicated
biological
processes,
tumorigenesis,
development,
and
wound
repair
amongst
other
processes
pathologies.
Recent
findings
reveal
that
senescent
can
both
promote
inhibit
cutaneous
healing
processes.
Relating
acute
chronic
wounds
will
help
to
clarify
their
role
inform
our
understanding
cell
heterogeneity.
To
this
apparent
contradiction
guide
future
research
therapeutic
we
review
rapidly
growing
cellular
its
biology.
Senescence
drives
the
onset
and
severity
of
multiple
ageing-associated
diseases
frailty.
As
a
result,
there
has
been
an
increased
interest
in
mechanistic
studies
search
for
compounds
targeting
senescent
cells,
known
as
senolytics.
Mammalian
models
are
commonly
used
to
test
senolytics
generate
functional
toxicity
data
at
level
organs
systems,
yet
this
is
expensive
time
consuming.
Zebrafish
share
high
homology
genes
associated
with
human
ageing
disease.
They
can
be
genetically
modified
relatively
easily.
In
larvae,
most
develop
within
5
days
fertilisation
transparent,
which
allows
tracking
fluorescent
cells
vivo
real
time,
testing
drug
off-target
assessment
cellular
phenotypic
changes.
Here,
we
have
generated
transgenic
zebrafish
line
that
expresses
green
protein
(GFP)
under
promoter
key
senescence
marker,
p21.
We
show
increase
p21:GFP
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 5, 2023
SUMMARY
Senescent
cells
drive
age-related
tissue
dysfunction
via
the
induction
of
a
chronic
senescence-associated
secretory
phenotype
(SASP).
The
cyclin-dependent
kinase
inhibitors
p21
Cip1
and
p16
Ink4a
have
long
served
as
markers
cellular
senescence.
However,
their
individual
roles
remain
incompletely
elucidated.
Thus,
we
conducted
comprehensive
examination
multiple
single-cell
RNA
sequencing
(scRNA-seq)
datasets
spanning
both
murine
human
tissues
during
aging.
Our
analysis
revealed
that
transcripts
demonstrate
significant
heterogeneity
across
distinct
cell
types
tissues,
frequently
exhibiting
lack
co-expression.
Moreover,
identified
tissue-specific
variations
in
SASP
profiles
linked
to
or
expression.
study
underscores
extraordinary
diversity
senescence
SASP,
emphasizing
these
phenomena
are
inherently
cell-
tissue-dependent.
few
factors
consistently
contribute
shared
“core”
SASP.
These
findings
highlight
need
for
more
nuanced
investigation
wide
array
biological
contexts.
