Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

Biology of Reproduction, Год журнала: 2024, Номер 110(6), С. 1135 - 1156

Опубликована: Апрель 19, 2024

Abstract Conventional, brightfield-microscopic semen analysis provides important baseline information about sperm quality of an individual; however, it falls short identifying subtle subcellular and molecular defects in cohorts “bad,” defective human animal spermatozoa with seemingly normal phenotypes. To bridge this gap, is desirable to increase the precision andrological evaluation humans livestock animals by pursuing advanced biomarker-based imaging methods. This review, spiced up occasional classic movie references but seriously scholastic at same time, focuses mainly on biomarkers altered male germ cell proteostasis resulting post-testicular carryovers proteins associated ubiquitin-proteasome system. Also addressed are redox homeostasis, epididymal maturation, sperm–seminal plasma interactions, surface glycosylation. Zinc ion homeostasis-associated sperm-borne components, including elements neurodegenerative pathways such as Huntington Alzheimer disease, discussed. Such spectrum biomarkers, imaged highly specific vital fluorescent probes, lectins, antibodies, reveals both obvious chromatin, deoxyribonucleic acid, accessory structures head tail. Introduction next-generation image-based flow cytometry into research clinical andrology will soon enable incorporation machine deep learning algorithms end point developing simple, label-free methods for diagnostics high-throughput phenotyping economically animals.

Язык: Английский

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Genetic Underpinnings of Oligoasthenoteratozoospermia

Clinical Genetics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

ABSTRACT Oligoasthenoteratozoospermia (OAT) is a frequent but severe type of male infertility. As one the most multifaceted infertility resulting from sperm problems, its genetic etiology remains unknown in cases. In this review, we systematically sort out latest literature on clinical reports and animal models leading to OAT, summarise expression profiles causative genes for highlight important role protein transport system during spermiogenesis, spermatid cell‐specific genes, Golgi acrosome‐related manchette‐related HTCA‐related axoneme‐related OAT development. These would be instrumental etiological screening, counseling, pre‐implantation testing patients with OAT.

Язык: Английский

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Brief biology and pathophysiology of Tekt bundles

Cell Adhesion & Migration, Год журнала: 2025, Номер 19(1)

Опубликована: Фев. 13, 2025

Tektins, a family of microtubule-stabilizing proteins, are critical for cilia and flagella assembly in mammals. They maintain doublet microtubule stability ciliary/flagellar motility. Loss Tekt1–5 causes instability, impaired motility, diseases like infertility, retinal degeneration, Mainzer-Saldino syndrome, diabetic nephropathy. Pathophysiological stimuli regulate Tektin expression through transcriptional, posttranscriptional, translational, posttranslational modifications. This review summarizes the latest findings on functions their role diseases.

Язык: Английский

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Chemosensory signalling in human sperm is controlled by Ca²⁺ influx via CatSper and Ca²⁺ clearance via plasma membrane Ca²⁺ ATPases

British Journal of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Loss of function the sperm-specific Ca2+ channel CatSper is a common channelopathy that causes male infertility. controls intracellular concentration and, thereby, motility human sperm. Activation by oviductal ligands evokes transient increase, which entails changes in flagellar beat are required for fertilisation. The CatSper-mediated influx has been studied extensively, whereas mechanisms underlying clearance and recovery from have remained ill-defined. We examined how pharmacological suppression export cytosol into extracellular space or uptake stores affects resting signals sperm healthy volunteers infertile men lacking functional channels, using kinetic Ca2+- pH-fluorometry as well patch-clamp recordings. show entering via predominantly, if not exclusively, exported plasma membrane ATPases (PMCAs). Na+/Ca2+ exchange mitochondria play no only negligible role signalling controlled interplay PMCAs, is, balance between

Язык: Английский

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Ion channels and transporters involved in calcium flux regulation in mammalian sperm

Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Bringing proteomics to bear on male fertility: key lessons

Expert Review of Proteomics, Год журнала: 2024, Номер 21(4), С. 181 - 203

Опубликована: Март 27, 2024

Introduction Male infertility is a major public health concern globally. Proteomics has revolutionized our comprehension of male fertility by identifying potential biomarkers and reproductive defects. Studies comparing sperm proteome with other tissues have the to refine diagnostics guide treatment development.

Язык: Английский

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The Odad3 Gene Is Necessary for Spermatozoa Development and Male Fertility in Mice

Cells, Год журнала: 2024, Номер 13(12), С. 1053 - 1053

Опубликована: Июнь 18, 2024

Odad3 gene loss-of-function mutation leads to Primary Ciliary Dyskinesia (PCD), a disease caused by motile cilia dysfunction. Previously, we demonstrated that knockout of the in mice replicates several features PCD, such as hydrocephalus, defects left–right body symmetry, and male infertility, with complete absence sperm reproductive tract. The majority animals die before sexual maturation due severe hydrocephalus failure thrive, which precludes fertility studies. Here, performed expression analysis during gonad development adult testes. We showed starts its first wave spermatogenesis, specifically at meiotic stage, is restricted germ cells testes, suggesting plays role spermatozoa formation. Subsequently, conditionally deleted males even partial ablation asthenoteratozoospermia multiple morphological abnormalities flagella (MMAF) mice. seminiferous tubules Odad3-deficient revealed spermatogenesis accumulation spermiogenesis spermiation phases. Furthermore, heterozygous Odad3+/− reduction count motility well abnormal morphology. Additionally, exhibited shorter fertile lifespan. Overall, these results suggest important dosage fertility. These findings may have an impact on genetic counseling practice PCD patients carrying mutations.

Язык: Английский

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Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system

Metabolic Engineering, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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Ether lipids and a peroxisomal riddle in sperm

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Май 15, 2023

Sperm are terminally differentiated cells that lack most of the membranous organelles, resulting in a high abundance ether glycerolipids found across different species. Ether lipids include plasmalogens, platelet activating factor, GPI-anchors and seminolipid. These play important roles sperm function performance, thus special interest as potential fertility markers therapeutic targets. In present article, we first review existing knowledge on relevance types for production, maturation function. To further understand ether-lipid metabolism sperm, then query available proteomic data from highly purified produce map metabolic steps retained these cells. Our analysis pinpoints presence truncated lipid biosynthetic pathway would be competent production precursors through initial peroxisomal core steps, but devoid subsequent microsomal enzymes responsible final synthesis all complex ether-lipids. Despite widely accepted notion peroxisomes, thorough published conducted herein identifies nearly 70% known resident proteins part proteome. view this, highlight open questions related to possible functions sperm. We propose repurposed role detoxification products oxidative stress, which is critically influence The likely peroxisomal-derived remnant compartment could act sink toxic fatty alcohols aldehydes generated by mitochondrial activity discussed. With this perspective, our provides comprehensive associated with ether-lipids peroxisomal-related offers new insights into potentially relevant antioxidant mechanisms warrant research.

Язык: Английский

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CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa.

PubMed, Год журнала: 2023, Номер 2023

Опубликована: Янв. 1, 2023

Centrosomes play an important role in the microtubule organization of a cell. The sperm's specialized centrosome consists canonical barrel-shaped proximal centriole, funnel-shaped distal and pericentriolar material known as striated columns (or segmented columns). Here, we examined localization centriole proteins CEP135 CP110 cattle human spermatozoa. In centrioles, is tip protein that controls cilia formation, while structural essential for constructing centriole. contrast, found antibodies recognizing label near centrioles at expected location capitulum humans antibody species-specific way. These findings provide pathway to understanding unique functions spermatozoan centrosome.

Язык: Английский

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