Biology,
Год журнала:
2023,
Номер
12(3), С. 409 - 409
Опубликована: Март 6, 2023
Ammonia
is
a
significant
concern
during
hatchery
culture
in
brachyuran
species,
and
its
accumulation
may
lead
to
abortive
moulting
large-scale
deaths
of
the
early
juveniles.
To
date,
underlying
mechanism
for
ammonia-induced
alteration
process
still
unknown.
In
this
study,
we
aimed
investigate
effects
ammonia
on
as
well
potential
mechanisms
juveniles
swimming
crab
Portunus
trituberculatus,
an
important
aquaculture
species
China.
We
evaluated
survival
rate
juvenile
crabs
(C2)
analyzed
expression
pattern
genes
key
components
molt
signaling
complete
cycle
under
different
concentrations
nitrogen
(the
control
group:
<0.1
mg/L;
LA
5
HA
20
mg/L).
The
results
showed
that:
(1)
groups
was
lower
than
that
group
at
end
experiment,
death
syndrome
(MDS)
only
observed
group;
(2)
higher
compared
(3)
consistent
with
MIH
decreased
expression,
related
ecdysteroid
synthesis,
receptors,
responsive
effectors
exhibited
increased
(4)
although
upregulated,
associated
receptors
downstream
group.
Our
indicated
low
levels
can
promote
by
inhibiting
activating
moult
signaling,
whereas
high
inhibit
MDS
through
impairing
signaling.
Frontiers in Marine Science,
Год журнала:
2023,
Номер
9
Опубликована: Фев. 8, 2023
Salinity
is
an
ecological
factor
affecting
the
physiology,
survival,
and
distribution
of
crustaceans.
Additionally,
salinity
fluctuation
detrimentally
affects
composition
biological
process
As
a
significant
commercial
aquaculture
species
in
China,
Japan,
Southeast
Asian
countries,
oriental
river
prawn,
Macrobrachium
nipponense,
can
tolerate
wide
range
salinity.
The
transcriptome,
proteome,
histology,
physiology
analysis
were
utilized
to
explore
physiological
responses
molecular
mechanisms
tolerance
M.
nipponense
.
Through
three-month
culture,
statistic
growth
trait
illustrated
relatively
excellent
performance
low
salinity,
higher
exposure
significantly
affected
In
terms
histological
analysis,
gills
hepatopancreas
suffered
varying
degrees
damage.
Besides,
activities
digestive,
immune-related,
metabolic
enzymes
calculated.
These
results
indicated
that
influenced
trypsin
amylase
hepatopancreas,
especially
14
ppt.
immune-related
activated
high
Notably,
activity
was
7
ppt,
which
testified
ppt
near
isotonic
point
gills,
muscle,
high-throughput
mRNA
sequencing
revealed
11356,
2227,
1819
differentially
expressed
genes
(DEGs)
by
comparing
7,
14,
21
groups
with
0ppt
group,
respectively.
TMT-labeling
proteome
identified
439
230
proteins
(DEPs)
through
comparison
0
integration
transcriptome
several
pathways
related
adaptation
enriched,
including
protein
export,
cGMP-PKG
signaling
pathway,
Amino
sugar
nucleotide
metabolism,
Glycine,
serine
threonine
metabolism.
16
up
down-regulated
DEGs
detected
KEGG
enrichment
ETHE1,
BIP,
chitinase
(E3.2.1.14),
SARDH.
no
regulated
recorded
correlation
hepatopancreas.
Thus,
optimum
survival
may
from
Overall,
these
provide
valuable
insights
into
underlying
culture
different
Animals,
Год журнала:
2023,
Номер
13(18), С. 2884 - 2884
Опубликована: Сен. 11, 2023
Salinity
is
an
important
factor
in
the
aquatic
environment
and
affects
ion
homeostasis
physiological
activities
of
crustaceans.
Macrobrachium
nipponense
a
shrimp
that
mainly
lives
fresh
low-salt
waters
plays
huge
economic
role
China's
market.
Currently,
there
are
only
few
studies
on
effects
salinity
M.
nipponense.
Therefore,
it
particular
importance
to
study
molecular
responses
fluctuations.
In
this
study,
was
set
at
salinities
0,
8,
14
22‱
for
6
weeks.
The
gills
from
control
(0‱)
isotonic
groups
(14‱)
were
used
RNA
extraction
transcriptome
analysis.
total,
593
differentially
expressed
genes
(DEGs)
identified,
which
282
up-regulated
311
down-regulated.
most
abundant
gill
transcripts
responding
different
levels
based
GO
classification
organelle
membrane
(cellular
component),
creatine
transmembrane
transporter
activity
(molecular
function)
transport
(biological
function).
KEGG
analysis
showed
enriched
significantly
affected
pathways
included
AMPK
signaling,
lysosome
cytochrome
P450.
addition,
15
DEGs
selected
qRT-PCR
verification,
related
homeostasis,
glucose
metabolism
lipid
metabolism.
results
expression
patterns
these
similar
high-throughput
data.
Compared
with
group,
high
caused
obvious
injury
tissue,
manifested
as
contraction
relaxation
filament,
cavity
vacuolation
severe
epithelial
disintegration.
Glucose-metabolism-related
enzyme
(e.g.,
pyruvate
kinase,
hexokinase,
6-phosphate
fructose
kinase)
related-gene
higher
14‱.
This
stress
activated
channels
promoted
level
High
damage
tissue
Overall,
improved
our
understanding
salt
tolerance
mechanism
Aquaculture Research,
Год журнала:
2019,
Номер
50(10), С. 2888 - 2895
Опубликована: Июль 2, 2019
A
30-day
experiment
was
conducted
to
investigate
the
effects
of
long-term
low-salinity
stress
on
growth
performance
and
osmotic
related
chlorine
ion
channel
ligand
regulation:
gamma-aminobutyric
acid
(GABA)
content
GABAA
receptor-associated
protein
(GABARAP)
expression
in
Portunus
trituberculatus.
The
salinity
levels
both
control
group
experimental
were
30
12
psu
respectively.
After
rearing
for
days,
specific
rate
survival
compared
between
two
groups,
6
used
test
tolerance.
results
as
follows:
(a)
Both
significant
lower
(p
<
0.05)
after
days;
(b)
challenge
with
72
hr,
crabs
had
a
100%
rate,
whereas
all
dead
within
48
hr;
(c)
GABA
gene
level
GABARAP
different
from
via
psu.
In
group,
increased
rapidly
9.96
±
2.09
42.00
5.94
µg/g;
however,
it
only
27.82
2.55
maximum
at
24
then
decreased
stabilized
suggesting
that
trigged
during
early
stage
resistance.
Biology,
Год журнала:
2023,
Номер
12(3), С. 409 - 409
Опубликована: Март 6, 2023
Ammonia
is
a
significant
concern
during
hatchery
culture
in
brachyuran
species,
and
its
accumulation
may
lead
to
abortive
moulting
large-scale
deaths
of
the
early
juveniles.
To
date,
underlying
mechanism
for
ammonia-induced
alteration
process
still
unknown.
In
this
study,
we
aimed
investigate
effects
ammonia
on
as
well
potential
mechanisms
juveniles
swimming
crab
Portunus
trituberculatus,
an
important
aquaculture
species
China.
We
evaluated
survival
rate
juvenile
crabs
(C2)
analyzed
expression
pattern
genes
key
components
molt
signaling
complete
cycle
under
different
concentrations
nitrogen
(the
control
group:
<0.1
mg/L;
LA
5
HA
20
mg/L).
The
results
showed
that:
(1)
groups
was
lower
than
that
group
at
end
experiment,
death
syndrome
(MDS)
only
observed
group;
(2)
higher
compared
(3)
consistent
with
MIH
decreased
expression,
related
ecdysteroid
synthesis,
receptors,
responsive
effectors
exhibited
increased
(4)
although
upregulated,
associated
receptors
downstream
group.
Our
indicated
low
levels
can
promote
by
inhibiting
activating
moult
signaling,
whereas
high
inhibit
MDS
through
impairing
signaling.
