Urine Metabolomics Reveals the Intervention Effects and Mechanism of Shenhua Tablets in IgA Nephropathy DOI

Yuhang Wang,

Ping Li,

Fengting Yin

и другие.

Biomedical Chromatography, Год журнала: 2025, Номер 39(5)

Опубликована: Апрель 7, 2025

ABSTRACT Shenhua tablets (SHT), a traditional Chinese medicine (TCM), have shown significant clinical efficacy in treating IgA nephropathy (IgAN), but the underlying mechanisms are not fully understood. This study aims to elucidate renoprotective effects of SHT on IgAN and explore potential its action using metabolomics approaches. The were evaluated Thy‐1 antibody‐induced rat model. Metabolomics techniques employed detect analyze urine biomarkers IgAN, identify targets metabolic pathways. significantly reduced levels 24‐h protein (Upro), albumin‐to‐creatinine ratio (ACR), Interleukin 1β (IL‐1β), tumor necrosis factor‐α (TNF‐α), interleukin 6 (IL‐6), alleviated kidney tissue damage, inhibited mesangial cell proliferation. Seventeen metabolites identified as for 14 which restored by SHT. primarily modulated pathways, including tricarboxylic acid (TCA) cycle, glycolysis/gluconeogenesis, pyruvate metabolism, β‐alanine upregulating citric succinic while downregulating pyruvic acid, L‐lactic uracil, malonic semialdehyde. exerts modulating key pathways normalizing abnormal levels.

Язык: Английский

Targeting Lactobacillus johnsonii to reverse chronic kidney disease DOI Creative Commons
Hua Miao, Fei Liu, Yanni Wang

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)

Опубликована: Авг. 5, 2024

Abstract Accumulated evidence suggested that gut microbial dysbiosis interplayed with progressive chronic kidney disease (CKD). However, no available therapy is effective in suppressing CKD. Here, using microbiomics 480 participants including healthy controls and patients stage 1–5 CKD, we identified an elongation taxonomic chain Bacilli-Lactobacillales-Lactobacillaceae- Lactobacillus - johnsonii correlated CKD progression, whose abundance strongly clinical markers. L. reduced rats adenine-induced supplementation ameliorated lesion. Serum indole-3-aldehyde (IAld), level negatively creatinine rats, decreased serum of induced unilateral ureteral obstruction (UUO) 5/6 nephrectomy (NX) as well late patients. Treatment IAld dampened lesion through aryl hydrocarbon receptor (AHR) signal or UUO, cultured 1-hydroxypyrene-induced HK-2 cells. Renoprotective effect was partially diminished AHR deficiency mice Our further data showed treatment attenuated by via increasing level. Taken together, targeting might reverse This study provides a deeper understanding how microbial-produced tryptophan metabolism affects host discovers potential pathways for prophylactic therapeutic treatments

Язык: Английский

Процитировано

24
Poria cocos: traditional uses, triterpenoid components and their renoprotective pharmacology DOI

Zhi-yuan Guo,

X. Wu,

Shui-juan Zhang

и другие.

Acta Pharmacologica Sinica, Год журнала: 2024, Номер unknown

Опубликована: Окт. 31, 2024

Язык: Английский

Процитировано

13
The nuclear factor kappa B signaling pathway is a master regulator of renal fibrosis DOI Creative Commons
Na Ren, Wenfeng Wang, Liang Zou

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 14

Опубликована: Янв. 16, 2024

Renal fibrosis is increasingly recognized as a global public health problem. Acute kidney injury (AKI) and chronic disease (CKD) both result in renal fibrosis. Oxidative stress inflammation play central roles progressive are closely linked form vicious cycle which oxidative induces through various molecular mechanisms. Ample evidence has indicated that hyperactive nuclear factor kappa B (NF-ƙB) signaling pathway plays pivotal role Hyperactive NF-ƙB causes the activation recruitment of immune cells. Inflammation, turn, triggers production reactive oxygen species nitrogen by activating leukocytes resident These events mediate organ apoptosis, necrosis, Therefore, developing strategy to target important for effective treatment This Review summarizes effect on context AKI CKD (immunoglobulin A nephropathy, membranous diabetic hypertensive transplantation). Therapies targeting pathway, including natural products, also discussed. In addition, NF-ƙB-dependent non-coding RNAs involved crucial targets development treatments disease. provides clear pathophysiological rationale specific concept-driven therapeutic pathway.

Язык: Английский

Процитировано

12
Targeted modulation of intestinal barrier and mucosal immune-related microbiota attenuates IgA nephropathy progression DOI Creative Commons
Ran Zhang,

Yuyan Tang,

Xiangru Feng

и другие.

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 28, 2025

IgA nephropathy (IgAN) is related to the balance of gut microbiota. However, it unclear whether changes in microbiota can cause IgAN or attenuate its progression. This study employed and human microbiota-associated (HMA)-IgAN models investigate impact on alteration mechanisms by which might trigger IgAN. Furthermore, this examined effects chitooligosaccharides (COS) COS formulation (COSF) with microbiota-targeting function enhancing intestinal barrier renal functions. These results revealed that led a reduction α-diversity structural alterations microbiota, characterized an increase Shigella sonnei, Streptococcus danieliae, Desulfovibrio fairfieldensis, decrease Bifidobacterium pseudolongum Clostridium leptum. There was also imbalance B-cell immunity level tight junction proteins (ZO-1 Occludin). Intestinal mucosal immune-related (Clostridium leptum, unclassified Lachnospiraceae NK4Al36 group, Clostridia vadinBB60 Oscillospiraceae, Roseburia) were enriched through targeted modulation COS/COSF, ZO-1 expression reducing APRIL/BAFF overexpression, thereby damage In conclusion, clarified kidney-gut crosstalk between IgAN, providing scientific evidence for developing microbiota-targeted food interventions improve outcomes.

Язык: Английский

Процитировано

2
Crosstalk between gut microbiotas and fatty acid metabolism in colorectal cancer DOI Creative Commons
Hao Zhang, Yuan Tian, Chunjie Xu

и другие.

Cell Death Discovery, Год журнала: 2025, Номер 11(1)

Опубликована: Фев. 26, 2025

Abstract Colorectal cancer (CRC) is the third most common malignancy globally and second leading cause of cancer-related mortality. Its development a multifactorial multistage process influenced by dynamic interplay between gut microbiota, environmental factors, fatty acid metabolism. Dysbiosis intestinal microbiota abnormalities in microbiota-associated metabolites have been implicated colorectal carcinogenesis, highlighting pivotal role microbial metabolic interactions. Fatty metabolism serves as critical nexus linking dietary patterns with activity, significantly impacting health. In CRC patients, reduced levels short-chain acids (SCFAs) SCFA-producing bacteria consistently observed. Supplementation probiotics has demonstrated tumor-suppressive effects, while therapeutic strategies aimed at modulating SCFA shown potential enhancing efficacy radiation therapy immunotherapy both preclinical clinical settings. This review explores intricate relationship metabolism, CRC, offering insights into underlying mechanisms their translational applications. Understanding this could pave way for novel diagnostic, therapeutic, preventive management CRC.

Язык: Английский

Процитировано

2
Fucoidan Oligosaccharide Supplementation Relieved Kidney Injury and Modulated Intestinal Homeostasis in D-Galactose-Exposed Rats DOI Open Access
Jing Shi, Yan Xu, Kening Zhang

и другие.

Nutrients, Год журнала: 2025, Номер 17(2), С. 325 - 325

Опубликована: Янв. 17, 2025

Background/Objectives: A fucoidan oligosaccharide (FOS), a potent compound derived from algae, is known for its diverse biological activities, including prebiotic activity, anticancer and antioxidative properties, has demonstrated supportive therapeutic effects in treating kidney ailments. This study was conducted to explore the protective influence of FOS on damage due aging induced by D-galactose Sprague Dawley (SD) rats. Methods: The low-dose group administered (100 mg/kg) gavage, high-FOS received (200 gavage. Results: findings showed that could effectively mitigate improve pathological condition tissues caused D-gal enhance function. Intervention with significantly reduced serum creatinine, uric acid, urea nitrogen levels, compared model group. mechanism D-gal-induced injury may be inhibit oxidative stress impaired mitochondrial function downregulating AMPK/ULK1 signaling pathway. also modulate expression autophagy-related proteins (Beclin-1, P62, LC3II/LC3I), thereby excessive mitophagy kidney. Furthermore, protect against preserving intestinal homeostasis. decreased lipopolysaccharide levels enhanced mucosal barrier upregulated abundances Bacteroidota, Muribaculaceae, Lactobacillus, while it Firmicutes, NK4A136_group, Lachnospiraceae_NK4A136_group. supplementation modulated gut microbiota composition, increasing beneficial bacteria reducing detrimental ones, potentially contributing improved Conclusions: safeguard renal D-gal-exposed rats inhibiting mitophagy, function, regulating

Язык: Английский

Процитировано

1
Aryl hydrocarbon receptor (AHR) and nuclear factor erythroid-derived 2-like 2 (NRF2): An important crosstalk in the gut-liver axis DOI Creative Commons

Rosanna Huchzermeier,

Emiel P. C. van der Vorst

Biochemical Pharmacology, Год журнала: 2025, Номер unknown, С. 116785 - 116785

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Gut microbiota‐derived tryptophan metabolites improve total parenteral nutrition‐associated infections by regulating Group 3 innate lymphoid cells DOI Creative Commons
Longchang Huang, Peng Wang, Shuai Liu

и другие.

iMeta, Год журнала: 2025, Номер 4(2)

Опубликована: Фев. 26, 2025

Abstract Clinical nutritional support is recognized by Klinefner's Surgery as one of the four pivotal advancements in surgical practice during 20th century. Surgeons regard clinical nutrition a “life‐saving” discipline, preserving lives numerous critically ill patients and facilitating success many procedures. Parenteral (PN) serves crucial component therapy, while range complications associated with total parenteral (TPN) can significantly undermine efficacy patient treatment. Impaired intestinal homeostasis strongly occurrence progression TPN‐related infections, yet underlying mechanisms remain poorly understood. In this study, RNA sequencing single‐cell (scRNA‐Seq) revealed that reduced secretion interleukin‐22 (IL‐22) Group 3 innate lymphoid cells (ILC3s) significant factor contributing to onset infections. Additionally, through 16S ribosomal (16S rRNA) gene gut microbiota from chronic failure metagenomic analysis mice, we observed TPN abundance Lactobacillus murinus ( L. ), supplementation could promote IL‐22 ILC3s. Mechanistically, upregulates indole‐3‐carboxylic acid, which activates nuclear receptor Rorγt stimulate This pathway strengthens barrier integrity reduces infection susceptibility. Our findings enhance our understanding driving highlighting critical role maintaining immune improving outcomes.

Язык: Английский

Процитировано

1
Bile acid metabolism in health and ageing-related diseases DOI
Xiaojun Li,

Chu Fang,

Ruihua Zhao

и другие.

Biochemical Pharmacology, Год журнала: 2024, Номер 225, С. 116313 - 116313

Опубликована: Май 22, 2024

Язык: Английский

Процитировано

7
Lung-Gut Microbiota and Tryptophan Metabolites Changes in Neonatal Acute Respiratory Distress Syndrome DOI Creative Commons
Jingli Yang, Yu He, Qing Ai

и другие.

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 3013 - 3029

Опубликована: Май 1, 2024

Purpose: Neonatal Acute Respiratory Distress Syndrome (NARDS) is a severe respiratory crisis threatening neonatal life.We aim to identify changes in the lung-gut microbiota and lung-plasma tryptophan metabolites NARDS neonates provide differentiated tool aid finding potential therapeutic targets.Patients Methods: Lower secretions, faeces plasma were collected from 50 including 25 patients (10 with mild NARDS_M group 15 moderate-to-severe NARDS_S group) control screened based on gestational age, postnatal age birth weight.Lower airway secretions feces underwent 16S rRNA gene sequencing understand microbial communities lung gut, while lower LC-MS analysis blood.Correlation analyses performed by comparing differences between control, groups.Results: Significant gut as well observed compared controls.Proteobacteria Bacteroidota increased lungs of neonates, whereas Firmicutes, Streptococcus, Rothia reduced.Lactobacillus decreased neonates.Indole-3-carboxaldehyde levels 3-hydroxykynurenine, indoleacetic acid, indolelactic 3-indole propionic indoxyl sulfate, kynurenine, neonates.Altered was significantly related metabolites, having better ability for diagnosis grading plasma.Conclusion: occurred neonates.Alterations discriminatory NARDS.

Язык: Английский

Процитировано

5