Journal of Functional Foods, Год журнала: 2025, Номер 128, С. 106842 - 106842
Опубликована: Апрель 25, 2025
Язык: Английский
Redox Biology, Год журнала: 2024, Номер 75, С. 103268 - 103268
Опубликована: Июль 17, 2024
Intracerebral hemorrhage (ICH) is a prevalent hemorrhagic cerebrovascular emergency. Alleviating neurological damage in the early stages of ICH critical for enhancing patient prognosis and survival rate. A novel form cell death called ferroptosis intimately linked to hemorrhage-induced brain tissue injury. Although studies have demonstrated significant preventive impact bovine serum albumin-stabilized selenium nanoparticles (BSA-SeNPs) against disorders connected system, neuroprotective effect on stroke mechanism remain unknown. Therefore, based favorable biocompatibility BSA-SeNPs, h-ICH (hippocampus-intracerebral hemorrhage) model was constructed perform BSA-SeNPs therapy. As expected, these could effectively improve cognitive deficits ameliorate hippocampal neuron. Furthermore, reverse morphology mitochondria enhanced mitochondrial function, evidenced by respiration function (OCR) membrane potential (MMP). Mechanistically, efficiently activate Nrf2 enhance expression antioxidant GPX4 at mRNA protein levels, further inhibit lipid peroxidation production erastin-induced ferroptotic damages. Taken together, this study not only sheds light clinical application but also provides its newly theoretical support strategy intervention treatment impairment following ICH.
Язык: Английский
Процитировано
13
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 2709 - 2732
Опубликована: Март 1, 2024
Purpose: Given the escalating prevalence of diabetes, demand for specific bone graft materials is increasing, owing to greater tendency towards defects and more difficult defect repair resulting from diabetic disease (DBD). Melatonin (MT), which known its potent antioxidant properties, has been shown stimulate both osteogenesis angiogenesis. Methods: MT was formulated into MT@PLGA nanoparticles (NPs), mixed with sodium alginate (SA) hydrogel, contained within a 3D printing polycaprolactone/β-Tricalcium phosphate (PCL/β-TCP) scaffold. The osteogenic capacity nanocomposite scaffold under conditions demonstrated via in vitro vivo studies underlying mechanisms were investigated. Results: Physicochemical characterization experiments confirmed successful fabrication scaffold, can achieve long-lasting sustained release MT. that exhibited enhanced capacity, elucidated by dual angiogenesis effects activated through NF-E2–related factor 2/Heme oxygenase 1 (Nrf2/HO-1) signaling pathway, including enhancement enzyme activity reduce oxidative stress damage vascular endothelial cells (VECs) directly stimulating growth (VEGF) production, reversed angiogenesis-osteogenesis uncoupling promoted conditions. Conclusion: This study research prospective clinical implications as novel treating enhancing fusion individuals. Keywords: defect, melatonin, Nrf2/HO-1 effects,
Язык: Английский
Процитировано
11
Biomolecules, Год журнала: 2024, Номер 14(8), С. 978 - 978
Опубликована: Авг. 9, 2024
Gouty arthritis (GA) is an inflammatory disease caused by monosodium urate (MSU) crystals deposited in the joint tissues causing severe pain. The can recur frequently and tends to form tophus joints. Current therapeutic drugs for acute phase of GA have many side effects limitations, are unable prevent recurrent attacks formation, overall efficacy unsatisfactory. Therefore, we need advance research on microscopic mechanism seek safer more effective through relevant targets block process. shows that pathogenesis closely related NLRP3 inflammation, oxidative stress, MAPK, NET, autophagy, Ferroptosis. However, after synthesizing sorting out above mechanisms, it found presence ROS throughout almost entire spectrum micro-mechanisms gout process, which combines multiple immune responses a large network diagram complex tight connections involved studies shown cell necrosis, pathological signs be effectively suppressed modulating network-related targets. In this article, one hand, investigated generative generation its association with GA. On other explored potential treatment prevention provide reference ideas development highly
Язык: Английский
Процитировано
7
Toxicology and Applied Pharmacology, Год журнала: 2024, Номер 486, С. 116940 - 116940
Опубликована: Апрель 25, 2024
Язык: Английский
Процитировано
5
Nature Metabolism, Год журнала: 2024, Номер 6(6), С. 1108 - 1127
Опубликована: Май 31, 2024
Abstract Oxygen is critical for all metazoan organisms on the earth and impacts various biological processes in physiological pathological conditions. While oxygen-sensing systems inducing acute hypoxic responses, including hypoxia-inducible factor pathway, have been identified, those operating prolonged hypoxia remain to be elucidated. Here we show that pyridoxine 5′-phosphate oxidase (PNPO), which catalyses bioactivation of vitamin B6, serves as an oxygen sensor regulates lysosomal activity macrophages. Decreased PNPO under reduced active form pyridoxal (PLP), inhibited acidification, macrophages led iron dysregulation, TET2 protein loss delayed resolution inflammatory response. Among PLP-dependent metabolism, supersulfide synthesis was suppressed hypoxia, resulting inhibition consequent proinflammatory phenotypes The PNPO–PLP axis creates a distinct layer sensing gradually shuts down metabolism response deprivation.
Язык: Английский
Процитировано
5
Journal of Orthopaedic Surgery and Research, Год журнала: 2024, Номер 19(1)
Опубликована: Июнь 28, 2024
Abstract Background Tendon stem/progenitor cell (TSPC) senescence contributes to tendon degeneration and impaired repair, resulting in age-related disorders. Ferroptosis, a unique iron-dependent form of programmed death, might participate the process senescence. However, whether ferroptosis plays role TSPC regeneration remains unclear. Recent studies reported that Platelet-derived exosomes (PL-Exos) provide significant advantages musculoskeletal inflammation regulation. The effects mechanism PL-Exos on are worthy further study. Methods Herein, we examined pathogenesis were isolated determined by TEM, particle size analysis, western blot mass spectrometry identification. We investigated function underlying mechanisms via blot, real-time quantitative polymerase chain reaction, immunofluorescence analysis vitro. was evaluated HE staining, Safranin-O biomechanical tests rotator cuff tear model rats. Results discovered involved senescent TSPCs. Furthermore, mitigated aging phenotypes TSPCs induced t-BHP preserved their proliferation tenogenic capacity. vivo animal results indicated improved tendon-bone healing properties mechanical strength. Mechanistically, activated AMPK phosphorylation downstream nuclear factor erythroid 2-related 2 (Nrf2)/glutathione peroxidase 4 (GPX4) signaling pathway, leading suppression lipid peroxidation. inhibition or GPX4 blocked protective effect against t-BHP-induced Conclusion In conclusion, play crucial aging. AMPK/Nrf2/GPX4 activation found inhibit ferroptosis, consequently Our provided new theoretical evidence for potential application restrain promote regeneration.
Язык: Английский
Процитировано
5
Journal of Ethnopharmacology, Год журнала: 2024, Номер 326, С. 117930 - 117930
Опубликована: Фев. 17, 2024
Язык: Английский
Процитировано
4
Phytomedicine, Год журнала: 2024, Номер 129, С. 155661 - 155661
Опубликована: Апрель 21, 2024
Gallbladder cancer (GBC) poses a significant risk to human health. Its development is influenced by numerous factors, particularly the homeostasis of reactive oxygen species (ROS) within cells. This crucial for tumor cell survival, and abnormal regulation ROS associated with occurrence progression many cancers. Dihydrotanshinone I (DHT I), biologically effective ingredient isolated from salvia miltiorrhiza, has exhibited cytotoxic properties against various cells inducing apoptosis. However, precise molecular mechanisms which dht exerts its effects remain unclear. To explore anti-tumor impact on GBC elucidate potential mechanisms. The proliferation cells, NOZ SGC-996, was assessed using assays, including CCK-8 assay, colony formation assay EdU staining. We also examined apoptosis, cycle progression, levels, alterations in mitochondrial membrane delve into intricate mechanism. Quantitative PCR (qPCR), immunofluorescence staining, Western blotting were performed evaluate target gene expression at both mRNA protein levels. correlation between nuclear factor erythroid 2-related 2 (Nrf2) kelch-like ECH-associated 1 (Keap1) co-immunoprecipitation. Finally, vivo effect investigated xenograft model gallbladder mice. Our research findings indicated that exerted inhibiting proliferation, disrupting potential, oxidative stress studies substantiated inhibition growth nude Mechanistically, primarily targeted Nrf2 promoting Keap1 mediated degradation kinase C (PKC) induced phosphorylation. leads suppression translocation reduction expression. Moreover, overexpression effectively counteracted I, while knockdown significantly enhanced inhibitory GBC. Meanwhile, PKC inhibitors import increased sensitivity treatment. Conversely, activators, proteasome inhibitors, antioxidants activators all antagonized apoptosis generation SGC-996 inhibited regulating Keap1-Nrf2 signaling pathway These insights provide strong rationale further investigation as therapeutic agent
Язык: Английский
Процитировано
4
Journal of Ovarian Research, Год журнала: 2024, Номер 17(1)
Опубликована: Апрель 25, 2024
Abstract Oxidative damage induced granulosa cells (GCs) apoptosis was considered as a significant cause of compromised follicle quality, antioxidants therapy has emerged potential method for improving endometriosis pregnancy outcomes. Here, we found that GCs from patients show increased oxidative stress level. Methyl 3,4-dihydroxybenzoate (MDHB), small molecule compound is extracted natural plants, reversed tert-butyl hydroperoxide (TBHP) damage. Therefore, the aim this study to assess protective effect MDHB and its mechanisms. TUNEL staining immunoblotting cleaved caspase-3/7/9 showed attenuated TBHP apoptosis. Mechanistically, treatment decreased cellular mitochondria ROS production, improved mitochondrial function by rescuing membrane (MMP) ATP production. Meanwhile, protein upregulated expression vital antioxidant transcriptional factor Nrf2 enzymes SOD1, NQO1 GCLC inhibited state, further beneficial oocytes embryos quality. may represent drug candidate in protecting endometriosis, which can improve outcomes endometriosis-associated infertility.
Язык: Английский
Процитировано
4
Plants, Год журнала: 2024, Номер 13(2), С. 198 - 198
Опубликована: Янв. 11, 2024
Epilobium hirsutum L., commonly known as hairy willowherb, is a perennial herbaceous plant native to Europe and Asia. In Romania, the genus includes 17 species that are used in folk medicine for various purposes. This study aimed investigate anti-inflammatory antitumor potential of optimized extract (EH) animal models. The first investigated properties EH model was carrageenan-induced paw inflammation. Wistar rats were divided into three groups: negative control, positive control treated with indomethacin, group extract. Oxidative stress markers, cytokine levels, protein expressions assessed. demonstrated comparable those group. second study, effects assessed using tumor Ehrlich ascites carcinoma. Swiss albino mice four negative, cyclophosphamide (Cph), Group 3 Cph extract, 4 alone. Samples from fluid, liver, heart analyzed evaluate oxidative stress, inflammation, cancer markers. showed reduction tumor-associated inflammation stress. Overall, exhibited promising models studied. These findings suggest its natural adjuvant therapeutic agent addressing induced by different pathologies.
Язык: Английский
Процитировано
3