Scientific Horizons,
Год журнала:
2023,
Номер
27(2), С. 32 - 42
Опубликована: Дек. 26, 2023
In
animal
breeding,
genetic
methods
have
become
the
basis
of
breeding
work
and
veterinary
diagnostics.
Therefore,
their
development
improvement
is
an
actual
direction
modern
science.
The
aim
presented
was
to
study
concentration
quality
nucleic
acids
obtained
from
venous
blood
cattle
for
further
studies.
For
this
purpose,
a
modified
method
phenolchloroform
extraction,
adapted
DNA
extraction
blood,
with
subsequent
spectrometric
determination
assessment
its
were
applied.
As
result
research,
it
found
that
average
material
isolated
146.5±14.98
ng/µl.
main
part
samples
–
more
than
93%
contained
in
range
50
200
At
same
time,
time
interval
between
by
ratio
optical
density
at
A260/A280
wavelengths
during
year
did
not
significant
changes
on
parameters.
used
acid
94%
allowed
obtaining
good
suitable
A
correlation
43%
(P<0.001)
quality.
coefficients
repetition
intra-laboratory
studies
results
analysis
level
97%
(P<0.001),
which
indicates
use
husbandry.
allows
animals
minimal
economic
costs
research
Biological reviews/Biological reviews of the Cambridge Philosophical Society,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 26, 2024
ABSTRACT
DNA
in
eukaryotic
genomes
is
under
constant
assault
from
both
exogenous
and
endogenous
sources,
leading
to
damage,
which
considered
a
major
molecular
driver
of
ageing.
Fortunately,
the
genome
central
exome
are
safeguarded
against
these
attacks
by
abundant
peripheral
non‐coding
DNA.
Non‐coding
codes
for
small
RNAs
that
inactivate
foreign
nucleic
acids
cytoplasm
physically
blocks
nucleus.
Damage
produced
during
such
blockage
removed
form
extrachromosomal
circular
(eccDNA)
through
pore
complexes.
Consequently,
serves
as
line
defence
damage.
The
total
amount
DNA/heterochromatin
declines
with
age,
resulting
decrease
physical
eccDNA
exclusion,
thus
an
increase
accumulation
damage
nucleus
ageing
age‐related
diseases.
Here,
we
summarize
recent
evidence
supporting
protective
role
healthy
pathological
states
argue
proximate
cause
genetic
Strategies
aimed
at
strengthening
could
potentially
offer
better
systematic
protection
dynamic
diverse
assaults,
reduce
burden
exome,
slow
ageing,
counteract
diseases
promote
healthier
life
individuals.
Italian Journal of Food Safety,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 15, 2024
The
growing
demand
for
transparency
in
the
food
industry
has
led
to
significant
advancements
meat
traceability.
Ensuring
authenticity
and
origin
of
products
is
critical
consumer
trust,
public
health,
compliance
with
regulations.
This
paper
reviews
recent
innovations
traceability,
a
focus
on
blockchain
technology
as
novel
approach
ensuring
Additionally,
advanced
methods
verifying
origin,
such
isotope
fingerprinting,
DNA
analysis,
spectroscopic
methods,
are
discussed.
role
voluntary
certification
schemes
enhancing
traceability
verification
also
explored.
findings
highlight
importance
integrating
cutting-edge
technologies
build
robust
transparent
supply
chain.
Biological reviews/Biological reviews of the Cambridge Philosophical Society,
Год журнала:
2023,
Номер
98(6)
Опубликована: Ноя. 5, 2023
The
journal
aims
to
cover
the
whole
field
of
biology,
in
particular
growth
areas
modern
biology.Articles
range
from
comprehensive
reviews
a
broad
research
shorter
articles
on
more
specialised
topics,
and
very
great
flexibility
content
presentation
is
allowed.Articles
are
pitched
at
level
for
experts
research,
but
Abstract
Telomeres
are
short
repetitive
DNA
sequences
capping
the
ends
of
chromosomes.
Telomere
shortening
occurs
during
cell
division
and
may
be
accelerated
by
oxidative
damage
or
ameliorated
telomere
maintenance
mechanisms.
Consequently,
length
changes
with
age,
which
was
recently
confirmed
in
a
large
meta‐analysis
across
vertebrates.
However,
based
on
correlation
between
it
concluded
that
can
used
as
tool
for
chronological
age
estimation
animals.
Correlation
should
not
confused
predictability,
current
data
studies
suggest
telomeres
cannot
to
reliably
predict
individual
age.
There
biological
reasons
why
there
is
variation
dynamics,
mainly
due
high
susceptibility
wide
range
environmental,
but
also
genetic
factors,
rendering
unfeasible
estimation.
The
use
largely
misguided
effort,
its
occasional
reappearance
literature
raises
concerns
will
mislead
resources
wildlife
conservation.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 9, 2023
Systematic
reviews
are
critical
summaries
of
the
exiting
literature
on
a
given
subject
and,
when
combined
with
meta-analysis,
provides
quantitative
synthesis
evidence
to
direct
and
inform
future
research.
Such
must,
however,
account
for
complex
sources
between
study
heterogeneity
possible
bias,
such
as
publication
bias.
This
paper
presents
methods
results
research
using
newly
developed
software
tool
called
ABCal
(version
1.0.2)
compute
assess
author
bias
in
literature,
providing
measure
effect
overrepresented
authors
introducing
overall
interpretation
literature.
includes
new
metric
referred
which
is
potential
biases
per
frequency
or
proportions
contributions
from
specific
considered.
The
able
significant
portion
observed
studies
included
meta-analyses.
A
meta-regression
measures
values
revealed
that
higher
levels
were
associated
while
lower
was
evident
measures.
Furthermore,
software’s
capabilities
analyse
authorship
produce
scientometric
plots
reveal
distinct
patterns
both
temporal
geographic
distributions
publications,
may
relate
any
Thus,
can
aid
researchers
gaining
deeper
understanding
landscape
assist
identifying
key
contributors
holistic
trends.
Scientometrics,
Год журнала:
2023,
Номер
129(1), С. 581 - 600
Опубликована: Ноя. 26, 2023
Abstract
Systematic
reviews
are
critical
summaries
of
the
exiting
literature
on
a
given
subject
and,
when
combined
with
meta-analysis,
provides
quantitative
synthesis
evidence
to
direct
and
inform
future
research.
Such
must,
however,
account
for
complex
sources
between
study
heterogeneity
possible
bias,
such
as
publication
bias.
This
paper
presents
methods
results
research
using
newly
developed
software
tool
called
ABCal
(version
1.0.2)
compute
assess
author
bias
in
literature,
providing
measure
effect
overrepresented
authors
introducing
overall
interpretation
literature.
includes
new
metric
referred
which
is
potential
biases
per
frequency
or
proportions
contributions
from
specific
considered.
The
able
significant
portion
observed
studies
included
meta-analyses.
A
meta-regression
measures
values
revealed
that
higher
levels
were
associated
while
lower
was
evident
measures.
Furthermore,
software's
capabilities
analyse
authorship
produce
scientometric
plots
reveal
distinct
patterns
both
temporal
geographic
distributions
publications,
may
relate
any
Thus,
can
aid
researchers
gaining
deeper
understanding
landscape
assist
identifying
key
contributors
holistic
trends.
Scientific Horizons,
Год журнала:
2023,
Номер
27(2), С. 32 - 42
Опубликована: Дек. 26, 2023
In
animal
breeding,
genetic
methods
have
become
the
basis
of
breeding
work
and
veterinary
diagnostics.
Therefore,
their
development
improvement
is
an
actual
direction
modern
science.
The
aim
presented
was
to
study
concentration
quality
nucleic
acids
obtained
from
venous
blood
cattle
for
further
studies.
For
this
purpose,
a
modified
method
phenolchloroform
extraction,
adapted
DNA
extraction
blood,
with
subsequent
spectrometric
determination
assessment
its
were
applied.
As
result
research,
it
found
that
average
material
isolated
146.5±14.98
ng/µl.
main
part
samples
–
more
than
93%
contained
in
range
50
200
At
same
time,
time
interval
between
by
ratio
optical
density
at
A260/A280
wavelengths
during
year
did
not
significant
changes
on
parameters.
used
acid
94%
allowed
obtaining
good
suitable
A
correlation
43%
(P<0.001)
quality.
coefficients
repetition
intra-laboratory
studies
results
analysis
level
97%
(P<0.001),
which
indicates
use
husbandry.
allows
animals
minimal
economic
costs
research
