Spectrophotometric method for determining the quantity and quality of DNA in animal breeding DOI Open Access
V. Antāne,

Yktiyar Sarybayev,

Askar Osserbay

et al.

Scientific Horizons, Journal Year: 2023, Volume and Issue: 27(2), P. 32 - 42

Published: Dec. 26, 2023

In animal breeding, genetic methods have become the basis of breeding work and veterinary diagnostics. Therefore, their development improvement is an actual direction modern science. The aim presented was to study concentration quality nucleic acids obtained from venous blood cattle for further studies. For this purpose, a modified method phenolchloroform extraction, adapted DNA extraction blood, with subsequent spectrometric determination assessment its were applied. As result research, it found that average material isolated 146.5±14.98 ng/µl. main part samples – more than 93% contained in range 50 200 At same time, time interval between by ratio optical density at A260/A280 wavelengths during year did not significant changes on parameters. used acid 94% allowed obtaining good suitable A correlation 43% (P<0.001) quality. coefficients repetition intra-laboratory studies results analysis level 97% (P<0.001), which indicates use husbandry. allows animals minimal economic costs research

Language: Английский

Climate change, age acceleration, and the erosion of fitness in polar bears DOI Creative Commons
Levi Newediuk, Evan S. Richardson, Brooke A. Biddlecombe

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 8, 2024

Abstract Climate change is increasingly disrupting evolved life history strategies and reducing population viability in wild species. Using estimates of epigenetic age acceleration, a cellular biomarker lifetime stress the expression age-related phenotypes, we found that polar bears aged approximately one year faster for each degree warming since 1960s. Age acceleration was also associated with reproducing early life, linking this process to well-established theory. However, evidence erosion fitness as aging accelerated temperatures increased. Finally, using large pedigree, adaptive potential our study zero. Global will soon reach levels currently experienced by Arctic species, which could impose widespread physiological costs limit capacities worldwide.

Language: Английский

Citations

0

Issue Information DOI Open Access
John T. Welch, Christopher J. Howe, Shinichi Nakagawa

et al.

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2023, Volume and Issue: 98(6)

Published: Nov. 5, 2023

The journal aims to cover the whole field of biology, in particular growth areas modern biology.Articles range from comprehensive reviews a broad research shorter articles on more specialised topics, and very great flexibility content presentation is allowed.Articles are pitched at level for experts research, but

Language: Английский

Citations

0

ABCal: a Python package for Author Bias Computation and Scientometric Plotting for Reviews and Meta-Analyses DOI Creative Commons
Louis‐Stéphane Le Clercq

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 9, 2023

Systematic reviews are critical summaries of the exiting literature on a given subject and, when combined with meta-analysis, provides quantitative synthesis evidence to direct and inform future research. Such must, however, account for complex sources between study heterogeneity possible bias, such as publication bias. This paper presents methods results research using newly developed software tool called ABCal (version 1.0.2) compute assess author bias in literature, providing measure effect overrepresented authors introducing overall interpretation literature. includes new metric referred which is potential biases per frequency or proportions contributions from specific considered. The able significant portion observed studies included meta-analyses. A meta-regression measures values revealed that higher levels were associated while lower was evident measures. Furthermore, software’s capabilities analyse authorship produce scientometric plots reveal distinct patterns both temporal geographic distributions publications, may relate any Thus, can aid researchers gaining deeper understanding landscape assist identifying key contributors holistic trends.

Language: Английский

Citations

0

ABCal: a Python package for author bias computation and scientometric plotting for reviews and meta-analyses DOI Creative Commons
Louis‐Stéphane Le Clercq

Scientometrics, Journal Year: 2023, Volume and Issue: 129(1), P. 581 - 600

Published: Nov. 26, 2023

Abstract Systematic reviews are critical summaries of the exiting literature on a given subject and, when combined with meta-analysis, provides quantitative synthesis evidence to direct and inform future research. Such must, however, account for complex sources between study heterogeneity possible bias, such as publication bias. This paper presents methods results research using newly developed software tool called ABCal (version 1.0.2) compute assess author bias in literature, providing measure effect overrepresented authors introducing overall interpretation literature. includes new metric referred which is potential biases per frequency or proportions contributions from specific considered. The able significant portion observed studies included meta-analyses. A meta-regression measures values revealed that higher levels were associated while lower was evident measures. Furthermore, software's capabilities analyse authorship produce scientometric plots reveal distinct patterns both temporal geographic distributions publications, may relate any Thus, can aid researchers gaining deeper understanding landscape assist identifying key contributors holistic trends.

Language: Английский

Citations

0

Telomere length is not a useful tool for chronological age estimation in animals DOI
Michael Le Pepke

BioEssays, Journal Year: 2023, Volume and Issue: 46(2)

Published: Dec. 4, 2023

Abstract Telomeres are short repetitive DNA sequences capping the ends of chromosomes. Telomere shortening occurs during cell division and may be accelerated by oxidative damage or ameliorated telomere maintenance mechanisms. Consequently, length changes with age, which was recently confirmed in a large meta‐analysis across vertebrates. However, based on correlation between it concluded that can used as tool for chronological age estimation animals. Correlation should not confused predictability, current data studies suggest telomeres cannot to reliably predict individual age. There biological reasons why there is variation dynamics, mainly due high susceptibility wide range environmental, but also genetic factors, rendering unfeasible estimation. The use largely misguided effort, its occasional reappearance literature raises concerns will mislead resources wildlife conservation.

Language: Английский

Citations

0

Spectrophotometric method for determining the quantity and quality of DNA in animal breeding DOI Open Access
V. Antāne,

Yktiyar Sarybayev,

Askar Osserbay

et al.

Scientific Horizons, Journal Year: 2023, Volume and Issue: 27(2), P. 32 - 42

Published: Dec. 26, 2023

In animal breeding, genetic methods have become the basis of breeding work and veterinary diagnostics. Therefore, their development improvement is an actual direction modern science. The aim presented was to study concentration quality nucleic acids obtained from venous blood cattle for further studies. For this purpose, a modified method phenolchloroform extraction, adapted DNA extraction blood, with subsequent spectrometric determination assessment its were applied. As result research, it found that average material isolated 146.5±14.98 ng/µl. main part samples – more than 93% contained in range 50 200 At same time, time interval between by ratio optical density at A260/A280 wavelengths during year did not significant changes on parameters. used acid 94% allowed obtaining good suitable A correlation 43% (P<0.001) quality. coefficients repetition intra-laboratory studies results analysis level 97% (P<0.001), which indicates use husbandry. allows animals minimal economic costs research

Language: Английский

Citations

0