Neutralizing IgG4 antibodies are a biomarker of sustained efficacy after peanut oral immunotherapy
Journal of Allergy and Clinical Immunology,
Год журнала:
2024,
Номер
153(6), С. 1611 - 1620.e7
Опубликована: Март 7, 2024
Язык: Английский
“Allergolds”: Gold Nanocluster-Based Bioconjugates of Food Allergens with Reduced Immunoglobulin E Binding
ACS Omega,
Год журнала:
2025,
Номер
10(9), С. 9040 - 9050
Опубликована: Фев. 28, 2025
Allergen-specific
immunotherapy
represents
the
only
method
of
achieving
a
lasting
reduction
in
severity
allergic
symptoms.
However,
need
to
expose
patients
allergens
which
they
are
sensitized
carries
risks.
One
solution
is
use
denatured
whereby
structure
allergenic
proteins
disrupted,
preventing
their
recognition
by
immunoglobulin
E
(IgE)
antibodies
and
thus
reducing
risk
adverse
reactions.
Denaturation
often
carried
out
using
chemical
cross-linking
generate
allergoids.
Gold
nanoclusters
(AuNCs)
emerging
as
versatile
tools
biotechnology
due
part
ability
conjugate
wide
range
biological
molecules.
Previous
works
have
described
formation
AuNC
egg
such
Gal
d
4
(lysozyme),
2
(ovalbumin),
whole
whites.
In
all
cases,
bioconjugation
disrupted
protein
structure,
allowing
for
biosensing
applications.
this
work,
we
hypothesize
that
these
AuNC-allergen
bioconjugates
could
be
used
"Allergolds",
chemically
altered
versions
analogous
traditional
allergoid
formulations.
Using
spectroscopic
techniques,
confirm
chicken
disrupts
when
generated
from
both
purified
This
structural
perturbation
was
found
resilient
conditions
successfully
human
IgE.
These
results
establish
Allergolds
potential
tool
generating
systematically
extracts.
Язык: Английский
Origins and diversity of pan-isotype human bone marrow plasma cells
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 10, 2024
Abstract
Bone
marrow
plasma
cells
(BMPCs)
produce
durable,
protective
IgM,
IgG,
and
IgA
antibodies,
in
some
cases,
pro-allergic
IgE
but
their
properties
sources
are
unclear.
We
charted
single
BMPC
transcriptional
clonal
heterogeneity
food-allergic
non-allergic
individuals
across
CD19
protein
expression
given
its
inverse
correlation
to
longevity.
Transcriptional
diversity
revealed
distinct
functional
profiles.
Additionally,
distribution
of
somatic
hypermutation
intraclonal
antibody
sequence
variance
suggest
that
CD19low
CD19high
BMPCs
arise
from
recalled
memory
germinal
center
B
cells,
respectively.
Most
were
peanut-allergic
individuals;
two
out
32
independent
donors
bound
peanut
antigens
vitro
vivo.
These
findings
shed
light
on
origins
highlight
the
bone
as
a
source
pathogenic
allergy.
Язык: Английский
A novel IgE epitope-specific antibodies-based sandwich ELISA for sensitive measurement of immunoreactivity changes of peanut allergen Ara h 2 in processed foods
Frontiers in Nutrition,
Год журнала:
2024,
Номер
11
Опубликована: Фев. 19, 2024
Background
Peanut
is
an
important
source
of
dietary
protein
for
human
beings,
but
it
also
recognized
as
one
the
eight
major
food
allergens.
Binding
IgE
antibodies
to
specific
epitopes
in
peanut
allergens
plays
roles
initiating
peanut-allergic
reactions,
and
Ara
h
2
widely
considered
most
potent
allergen
best
predictor
allergy.
Therefore,
can
serve
useful
biomarkers
prediction
IgE-binding
variations
foods.
This
study
aimed
develop
validate
epitope-specific
(IgE-EsAbs)-based
sandwich
ELISA
(sELISA)
detection
measurement
IgE-immunoreactivity
changes
Methods
DEAE-Sepharose
Fast
Flow
anion-exchange
chromatography
combining
with
SDS-PAGE
gel
extraction
were
applied
purify
from
raw
peanut.
Hybridoma
epitope
vaccine
techniques
employed
generate
a
monoclonal
antibody
against
polyclonal
12
2,
respectively.
was
carried
out
evaluate
target
binding
specificity
generated
IgE-EsAbs.
Subsequently,
IgE-EsAbs-based
sELISA
developed
detect
its
allergenic
residues
samples.
The
capacity
foods
determined
by
competitive
ELISA.
dose-effect
relationship
between
content
(or
peanut)
ability
further
established
reliability
measuring
Results
obtained
had
purity
94.44%.
Antibody
characterization
revealed
that
IgE-EsAbs
epitope(s)
exhibited
high
specificity.
Accordingly,
using
these
able
samples,
sensitivity
(a
limit
0.98
ng/mL),
accuracy
mean
bias
0.88%),
precision
(relative
standard
deviation
<
16.50%),
specificity,
recovery
(an
average
98.28%).
Moreover,
could
predict
foods,
verified
sera
derived
individuals.
Conclusion
novel
immunoassay
be
user-friendly
method
monitor
low
level
preliminary
vitro
potential
allergenicity
processed
Язык: Английский
Mutation of Disulfide Bond Sites Reduces the Immunoreactivity of Cra a 4 by Changing the Structural Characteristics
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 10, 2024
Sarcoplasmic
calcium-binding
protein
(Cra
a
4)
from
Crassostrea
angulata
belongs
to
the
EF-hand
superfamily,
and
understanding
of
its
structure–allergenicity
relationship
is
still
insufficient.
In
this
study,
chemical
denaturants
were
used
destroy
structure
Cra
4,
showing
that
disruption
reduced
IgG-/IgE-binding
activity.
To
explore
which
critical
amino
acid
site
affects
allergenicity
mutants
obtained
by
site-directed
mutations
in
disulfide
bonds
(C97),
conformational
epitopes
(I105,
D114),
or
Ca2+-binding
region
(D106,
D110)
their
activity
was
significantly
using
serological
tests.
Notably,
C97A
had
lowest
immunoreactivity.
addition,
two
4
verified.
Meanwhile,
increase
α-helical
content,
surface
hydrophobicity,
electrostatic
potential
affected
allergenicity.
Overall,
allowed
development
hypoallergenic
mutant.
Язык: Английский
Trust your gut: Establishing confidence in gastrointestinal models - An overview of the state of the science and contexts of use
Susan J. Debad,
David Allen,
Omari J. Bandele
и другие.
ALTEX,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
The
webinar
series
and
workshop
titled
“Trust
Your
Gut:
Establishing
Confidence
in
Gastrointestinal
Models
–
An
Overview
of
the
State
Science
Contexts
Use”
was
co-organized
by
NICEATM,
NIEHS,
FDA,
EPA,
CPSC,
DoD,
Johns
Hopkins
Center
for
Alternatives
to
Animal
Testing
(CAAT)
hosted
at
National
Institutes
Health
Bethesda,
MD,
USA
on
October
11-12,
2023.
New
approach
methods
(NAMs)
assessing
issues
gastrointestinal
tract
(GIT)-
related
toxicity
offer
promise
addressing
some
limitations
associated
with
animal-based
assessments.
GIT
NAMs
vary
complexity,
from
two-dimensional
monolayer
cell
line-based
systems
sophisticated
3-dimensional
organoid
derived
human
primary
cells.
Despite
advances
NAMs,
challenges
remain
fully
replicating
complex
interactions
processes
occurring
within
GIT.
Presentations
discussions
addressed
regulatory
needs,
challenges,
innovations
incorporating
into
risk
assessment
frameworks;
explored
state
science
using
evaluating
systemic
toxicity,
understanding
absorption
pharmacokinetics,
potential
allergenicity;
discussed
strengths,
limitations,
data
gaps
as
well
steps
needed
establish
confidence
these
models
use
setting.
Язык: Английский
The Potential of Human Monoclonal IgE Antibodies to Establish Biological Potency and Stability of Allergen Extracts
Current Allergy and Asthma Reports,
Год журнала:
2024,
Номер
24(9), С. 471 - 475
Опубликована: Июль 24, 2024
Abstract
Purpose
of
Review
Allergenic
extracts
are
often
standardized
to
control
for
potency,
either
by
measuring
concentrations
major
allergens
or
“overall
allergenicity”
competition
IgE
in
pooled
sera
from
highly
allergic
subjects
with
a
reference
extract.
Recent
developments
present
an
opportunity
use
human
mAb
cloned
define
potency
allergenic
extracts.
Findings
Two
recent
revising
measurements
allergen
extracts:
cloning
specific
and
extensive
epitope
mapping
proteins.
Summary
Because
recognize
biologically
relevant
epitopes,
they
novel
determine
the
potencies
may
contribute
science
base
standardization.
Язык: Английский