The Potential of Human Monoclonal IgE Antibodies to Establish Biological Potency and Stability of Allergen Extracts DOI Creative Commons
Ronald L. Rabin

Current Allergy and Asthma Reports, Journal Year: 2024, Volume and Issue: 24(9), P. 471 - 475

Published: July 24, 2024

Abstract Purpose of Review Allergenic extracts are often standardized to control for potency, either by measuring concentrations major allergens or “overall allergenicity” competition IgE in pooled sera from highly allergic subjects with a reference extract. Recent developments present an opportunity use human mAb cloned define potency allergenic extracts. Findings Two recent revising measurements allergen extracts: cloning specific and extensive epitope mapping proteins. Summary Because recognize biologically relevant epitopes, they novel determine the potencies may contribute science base standardization.

Language: Английский

Neutralizing IgG4 antibodies are a biomarker of sustained efficacy after peanut oral immunotherapy DOI
Tarun Keswani,

Nicole A. LaHood,

Orlee Marini‐Rapoport

et al.

Journal of Allergy and Clinical Immunology, Journal Year: 2024, Volume and Issue: 153(6), P. 1611 - 1620.e7

Published: March 7, 2024

Language: Английский

Citations

10
“Allergolds”: Gold Nanocluster-Based Bioconjugates of Food Allergens with Reduced Immunoglobulin E Binding DOI Creative Commons

Victor Martinez-Macias,

Scott A. Gabel,

Craig Bennett

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(9), P. 9040 - 9050

Published: Feb. 28, 2025

Allergen-specific immunotherapy represents the only method of achieving a lasting reduction in severity allergic symptoms. However, need to expose patients allergens which they are sensitized carries risks. One solution is use denatured whereby structure allergenic proteins disrupted, preventing their recognition by immunoglobulin E (IgE) antibodies and thus reducing risk adverse reactions. Denaturation often carried out using chemical cross-linking generate allergoids. Gold nanoclusters (AuNCs) emerging as versatile tools biotechnology due part ability conjugate wide range biological molecules. Previous works have described formation AuNC egg such Gal d 4 (lysozyme), 2 (ovalbumin), whole whites. In all cases, bioconjugation disrupted protein structure, allowing for biosensing applications. this work, we hypothesize that these AuNC-allergen bioconjugates could be used "Allergolds", chemically altered versions analogous traditional allergoid formulations. Using spectroscopic techniques, confirm chicken disrupts when generated from both purified This structural perturbation was found resilient conditions successfully human IgE. These results establish Allergolds potential tool generating systematically extracts.

Language: Английский

Citations

0
Origins and diversity of pan-isotype human bone marrow plasma cells DOI Creative Commons
Gaspar A. Pacheco, Vishal Rao, Duck Kyun Yoo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 10, 2024

Abstract Bone marrow plasma cells (BMPCs) produce durable, protective IgM, IgG, and IgA antibodies, in some cases, pro-allergic IgE but their properties sources are unclear. We charted single BMPC transcriptional clonal heterogeneity food-allergic non-allergic individuals across CD19 protein expression given its inverse correlation to longevity. Transcriptional diversity revealed distinct functional profiles. Additionally, distribution of somatic hypermutation intraclonal antibody sequence variance suggest that CD19low CD19high BMPCs arise from recalled memory germinal center B cells, respectively. Most were peanut-allergic individuals; two out 32 independent donors bound peanut antigens vitro vivo. These findings shed light on origins highlight the bone as a source pathogenic allergy.

Language: Английский

Citations

3
A novel IgE epitope-specific antibodies-based sandwich ELISA for sensitive measurement of immunoreactivity changes of peanut allergen Ara h 2 in processed foods DOI Creative Commons

Yan Yan,

Liming Li,

Caiyun Long

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Feb. 19, 2024

Background Peanut is an important source of dietary protein for human beings, but it also recognized as one the eight major food allergens. Binding IgE antibodies to specific epitopes in peanut allergens plays roles initiating peanut-allergic reactions, and Ara h 2 widely considered most potent allergen best predictor allergy. Therefore, can serve useful biomarkers prediction IgE-binding variations foods. This study aimed develop validate epitope-specific (IgE-EsAbs)-based sandwich ELISA (sELISA) detection measurement IgE-immunoreactivity changes Methods DEAE-Sepharose Fast Flow anion-exchange chromatography combining with SDS-PAGE gel extraction were applied purify from raw peanut. Hybridoma epitope vaccine techniques employed generate a monoclonal antibody against polyclonal 12 2, respectively. was carried out evaluate target binding specificity generated IgE-EsAbs. Subsequently, IgE-EsAbs-based sELISA developed detect its allergenic residues samples. The capacity foods determined by competitive ELISA. dose-effect relationship between content (or peanut) ability further established reliability measuring Results obtained had purity 94.44%. Antibody characterization revealed that IgE-EsAbs epitope(s) exhibited high specificity. Accordingly, using these able samples, sensitivity (a limit 0.98 ng/mL), accuracy mean bias 0.88%), precision (relative standard deviation < 16.50%), specificity, recovery (an average 98.28%). Moreover, could predict foods, verified sera derived individuals. Conclusion novel immunoassay be user-friendly method monitor low level preliminary vitro potential allergenicity processed

Language: Английский

Citations

1
Mutation of Disulfide Bond Sites Reduces the Immunoreactivity of Cra a 4 by Changing the Structural Characteristics DOI
Shuai Gao,

Fei Huan,

Mingxuan Wu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: April 10, 2024

Sarcoplasmic calcium-binding protein (Cra a 4) from Crassostrea angulata belongs to the EF-hand superfamily, and understanding of its structure–allergenicity relationship is still insufficient. In this study, chemical denaturants were used destroy structure Cra 4, showing that disruption reduced IgG-/IgE-binding activity. To explore which critical amino acid site affects allergenicity mutants obtained by site-directed mutations in disulfide bonds (C97), conformational epitopes (I105, D114), or Ca2+-binding region (D106, D110) their activity was significantly using serological tests. Notably, C97A had lowest immunoreactivity. addition, two 4 verified. Meanwhile, increase α-helical content, surface hydrophobicity, electrostatic potential affected allergenicity. Overall, allowed development hypoallergenic mutant.

Language: Английский

Citations

1
Trust your gut: Establishing confidence in gastrointestinal models - An overview of the state of the science and contexts of use DOI Creative Commons

Susan J. Debad,

David Allen,

Omari J. Bandele

et al.

ALTEX, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The webinar series and workshop titled “Trust Your Gut: Establishing Confidence in Gastrointestinal Models – An Overview of the State Science Contexts Use” was co-organized by NICEATM, NIEHS, FDA, EPA, CPSC, DoD, Johns Hopkins Center for Alternatives to Animal Testing (CAAT) hosted at National Institutes Health Bethesda, MD, USA on October 11-12, 2023. New approach methods (NAMs) assessing issues gastrointestinal tract (GIT)- related toxicity offer promise addressing some limitations associated with animal-based assessments. GIT NAMs vary complexity, from two-dimensional monolayer cell line-based systems sophisticated 3-dimensional organoid derived human primary cells. Despite advances NAMs, challenges remain fully replicating complex interactions pro­cesses occurring within GIT. Presentations discussions addressed regulatory needs, challenges, innovations incorporating into risk assessment frameworks; explored state science using evaluating systemic toxicity, understanding absorption pharmacokinetics, potential allergenicity; discussed strengths, limitations, data gaps as well steps needed establish confidence these models use setting.

Language: Английский

Citations

0
The Potential of Human Monoclonal IgE Antibodies to Establish Biological Potency and Stability of Allergen Extracts DOI Creative Commons
Ronald L. Rabin

Current Allergy and Asthma Reports, Journal Year: 2024, Volume and Issue: 24(9), P. 471 - 475

Published: July 24, 2024

Abstract Purpose of Review Allergenic extracts are often standardized to control for potency, either by measuring concentrations major allergens or “overall allergenicity” competition IgE in pooled sera from highly allergic subjects with a reference extract. Recent developments present an opportunity use human mAb cloned define potency allergenic extracts. Findings Two recent revising measurements allergen extracts: cloning specific and extensive epitope mapping proteins. Summary Because recognize biologically relevant epitopes, they novel determine the potencies may contribute science base standardization.

Language: Английский

Citations

0